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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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First Agar Work - Cottony Growth
    #25624792 -

Hey guys, it's my first agar work. Inoculated 3 plates with B+ spore swab. 7 plates waiting in the fridge for healthy culture. Now my question is when should I isolate them? What is the yellow spot?


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Re: First Agar Work - Cottony Growth [Re: pandahaze]
    #25624797 -

The satellite colonies are contams, the center is B+ mycelium. In a still air box, cut a piece of clean mycelium and place it on a new Petri dish.

Edited by Thesunbeam (11/19/18 08:36 AM)

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Re: First Agar Work - Cottony Growth [Re: Thesunbeam]
    #25624806 -

Sector the fastes growth, make sure this portion is also uniform with no thin spots and what not.

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Re: First Agar Work - Cottony Growth [Re: Thesunbeam]
    #25624824 -

Thesunbeam said:
The satellite colonies are contams, the center is B+ mycelium. In a still air box, cut a piece of clean mycelium and place it on a new Petri dish.


Satellite
colony is the one that is on the edge of agar? So is is time to isolate or I should wait little bit more?

Edited by pandahaze (11/19/18 08:47 AM)

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Re: First Agar Work - Cottony Growth [Re: Thesunbeam]
    #25624831 -

Thesunbeam said:
Sector the fastes growth, make sure this portion is also uniform with no thin spots and what not.


What do you mean by sector the fastest growth? Sorry, I'm really new to agar work.

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Re: First Agar Work - Cottony Growth [Re: pandahaze]
    #25624840 -

Take a sample of the fastest clean growth free from the slower growth. Transfer this sample to a fresh plate of agar. Repeat again then either repeat and/or inoculate  grain.


--------------------
Never give up, never surrender.

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Re: First Agar Work - Cottony Growth [Re: Failboat]
    #25633822 -

Quirkmeister92 said:
Take a sample of the fastest clean growth free from the slower growth. Transfer this sample to a fresh plate of agar. Repeat again then either repeat and/or inoculate  grain.


Thanks for the reply. I did the transfers 3 days ago.  It seems this time growth might be rhizomorphic. Contams also began to show themselves on inoculated agars.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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