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InvisibleOrganic Chemist
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Registered: 04/10/17
Posts: 126
Spore germination
    #25584576 - 11/01/18 02:47 PM (6 years, 2 months ago)

Hey, I’m working on my first grow.  I inoculated 5 dishes of oat water agar on 26 OCT with PE6 from a spore print.  They’ve been in the lab at 78f since then with no signs of germination or contamination.  How long should it take before I see signs of life?

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Offlinetryingtotrip
Stranger

Registered: 09/26/18
Posts: 57
Last seen: 6 years, 1 month
Re: Spore germination [Re: Organic Chemist]
    #25584606 - 11/01/18 03:00 PM (6 years, 2 months ago)

how long has it been? average for first sign is 3-5 days but wait for up to two weeks. Are the spores fresh or old?

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InvisibleOrganic Chemist
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Registered: 04/10/17
Posts: 126
Re: Spore germination [Re: tryingtotrip]
    #25584640 - 11/01/18 03:14 PM (6 years, 2 months ago)

Quote:

tryingtotrip said:
how long has it been? average for first sign is 3-5 days but wait for up to two weeks. Are the spores fresh or old?



The plates were inoculated 26 OCT, so 6 days now.  Spore print was from freespores and used the day after it arrived, so I assume it’s fairly fresh.  If it may take two weeks, I guess I’m in good shape.  I’m definitely pleased to see zero signs of contamination so far!

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OfflinePopaCap
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Registered: 07/23/18
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Re: Spore germination [Re: tryingtotrip]
    #25584644 - 11/01/18 03:16 PM (6 years, 2 months ago)

Sometimes things just don’t work out, even with fresh spores. A few months back, I put Huautla spores to 8 plates from a trusted vendor syringe. Not a single one germinated. I said fuck it, expanded my syringe with a couple more cc’s of sterile water and knocked up 10 brf jars. All turned out great. I’d wait the 2 weeks, but if there’s no signs of life after a week, there probably won’t be. But who knows, stranger things have happened :justdontknow:


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InvisibleOrganic Chemist
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Registered: 04/10/17
Posts: 126
Re: Spore germination [Re: PopaCap]
    #25584661 - 11/01/18 03:24 PM (6 years, 2 months ago)

Quote:

PopaCap said:
Sometimes things just don’t work out, even with fresh spores. A few months back, I put Huautla spores to 8 plates from a trusted vendor syringe. Not a single one germinated. I said fuck it, expanded my syringe with a couple more cc’s of sterile water and knocked up 10 brf jars. All turned out great. I’d wait the 2 weeks, but if there’s no signs of life after a week, there probably won’t be. But who knows, stranger things have happened :justdontknow:



Gotcha.  They have a 30 day guarantee, so I think they’ll send another print if this one fails.

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OfflineWeavieWonder
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Registered: 08/18/16
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Re: Spore germination [Re: PopaCap]
    #25584788 - 11/01/18 04:09 PM (6 years, 2 months ago)

Quote:

PopaCap said:
Sometimes things just don’t work out, even with fresh spores. A few months back, I put Huautla spores to 8 plates from a trusted vendor syringe. Not a single one germinated.




For some reason, spores from a syringe do not germinate well on agar.  OP should see signs of life any day now.  Don't start worrying unless it's been more than 2 weeks.

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OfflinePopaCap
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Re: Spore germination [Re: WeavieWonder]
    #25584836 - 11/01/18 04:22 PM (6 years, 2 months ago)

I was aware they didn’t do that great, but I was expecting a little better results than 0 for 8, lol.


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Offlinecelkid
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Registered: 01/13/15
Posts: 134
Last seen: 5 years, 11 months
Re: Spore germination [Re: PopaCap]
    #25585060 - 11/01/18 05:45 PM (6 years, 2 months ago)

The spores also bunch up so shake well before inoculating


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OfflinePopaCap
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Re: Spore germination [Re: celkid]
    #25585077 - 11/01/18 05:51 PM (6 years, 2 months ago)

The OP is working off a print, not a syringe.


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Offlinecelkid
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Registered: 01/13/15
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Re: Spore germination [Re: PopaCap]
    #25585089 - 11/01/18 05:57 PM (6 years, 2 months ago)

My bad  :punish2:


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Offlinefull_wave
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Registered: 03/04/18
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Last seen: 3 years, 10 months
Re: Spore germination [Re: PopaCap]
    #25585320 - 11/01/18 07:26 PM (6 years, 2 months ago)

Quote:

PopaCap said:
Sometimes things just don’t work out, even with fresh spores. A few months back, I put Huautla spores to 8 plates from a trusted vendor syringe. Not a single one germinated. I said fuck it, expanded my syringe with a couple more cc’s of sterile water and knocked up 10 brf jars. All turned out great. I’d wait the 2 weeks, but if there’s no signs of life after a week, there probably won’t be. But who knows, stranger things have happened :justdontknow:



Man I had the same thing happen with Huautla but only with MEA, been like 28 days lol. I wonder if Huaulta just doesn't like going from spores to agar.

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InvisibleOrganic Chemist
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Registered: 04/10/17
Posts: 126
Re: Spore germination [Re: Organic Chemist]
    #25603290 - 11/09/18 02:23 PM (6 years, 2 months ago)

It’s been two weeks now with no growth, so I’m going to contact freespores for a replacement.  I’m assuming they’ll honor their guarantee and send a viable replacement.

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InvisibleOrganic Chemist
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Registered: 04/10/17
Posts: 126
Re: Spore germination [Re: Organic Chemist]
    #25623226 - 11/18/18 12:52 PM (6 years, 1 month ago)

Freespores never responded so it looks like I’m just out the money for the bad spore print.  Do you guys have any suggestions for a reputable vendor?

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OfflineCzM8
Kung Foo Grip
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Registered: 05/25/18
Posts: 773
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Re: Spore germination [Re: Organic Chemist]
    #25623512 - 11/18/18 03:31 PM (6 years, 1 month ago)

Check the market place. Surely someone there will trade you something. Post up there what your looking for if there isn't anything up yet.


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