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OfflineColibrii
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Registered: 10/14/18
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Spore syringe - sterilize flask along with water?
    #25621249 - 11/17/18 03:28 PM (6 years, 1 month ago)

Hi!
I'm going to make my first syringe from a spore print and would appreciate some advice on sterilization.

Let's say I have an Erlenmeyer flask with some water in it and I cover it with a small petri dish. Then I wrap it in a tin foil, put it in a pot and steam-sterilize for 60-90 minutes.
At this point, can I consider both the flask and the water sterile?

The idea is to avoid pouring pre-sterilized water into pre-sterilized flask thus exposing it to air contams.

Thank you.

PS: Please understand I'm quite new to this.

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OfflineApc123
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Registered: 04/26/18
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Re: Spore syringe - sterilize flask along with water? [Re: Colibrii]
    #25621279 - 11/17/18 03:40 PM (6 years, 1 month ago)

I just use a mason jar to sterile my water in. I like to use distilled water if I'm going to keep them around for a long time... But I also use tap water too. I put water in a mason jar that has an injection port and pc for 20-30 min at 15 psi. U can either use another small mason jar, a shot glass or even a sandwich baggie (which I have done lately) to put the water in when make the syringe. Extract the water sterile water once it has cooled completely with your sterile syringe and squirt it into one of the items listed above. Then scratch some spores off into the water and suck it up into the syringe :smile: good luck. Its scary the first time but have confidence! I have never messed up a syringe not even my first couple.
P.s. I like to test my syringes on a brf jar the first time instead of/or also with agar so then I can fruit it and get some more prints :smile:


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