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OfflineNN-TNT
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Registered: 08/04/18
Posts: 15
Last seen: 4 years, 3 months
g2g Bacterial blasphemy Exhausted morning post, don't mind me...
    #25620997 - 11/17/18 01:09 PM (6 years, 2 months ago)

I'm having issues with bacteria showing up on my wheat berries, could i possibly have made the jars too wet in my initial grain prep? I'm quite worried about the oat jars I just transferred to, and I am hoping bacteria won't hitch a ride along..

I'm tired, and ranting, but I'll post photos and a desc. of each one right away.

Basically, what I think I am asking, is what all causes bacterial contams/stalling, and what one can do to avoid it?


-Temp above 80F
-Too wet of grain prep
-?

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OfflineNN-TNT
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Registered: 08/04/18
Posts: 15
Last seen: 4 years, 3 months
Re: g2g Bacterial blasphemy Exhausted morning post, don't mind me... [Re: NN-TNT]
    #25621018 - 11/17/18 01:21 PM (6 years, 2 months ago)

https://www.shroomery.org/forums/showflat.php/Number/25585192/page/1

This post seems related, and it doesn't seem like the user suggested a fix

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InvisibleShroomspective
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I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: g2g Bacterial blasphemy Exhausted morning post, don't mind me... [Re: NN-TNT] * 1
    #25622389 - 11/18/18 05:51 AM (6 years, 1 month ago)

Bad technique generally..If you inoculate directly with spores, don't make enough transfers on agar to ensure its clean, poor quality LC/LI, don't have a good sterile procedure, don't PC for long enough..any of these basically

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OfflineNN-TNT
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Registered: 08/04/18
Posts: 15
Last seen: 4 years, 3 months
Re: g2g Bacterial blasphemy Exhausted morning post, don't mind me... [Re: Shroomspective]
    #25622931 - 11/18/18 11:00 AM (6 years, 1 month ago)

Could it be just my grains being prepped too wet? More likely possibly due to using spore 2 grain inoculation?

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InvisibleShroomspective
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Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: g2g Bacterial blasphemy Exhausted morning post, don't mind me... [Re: NN-TNT]
    #25623232 - 11/18/18 12:54 PM (6 years, 1 month ago)

There's you issue..spore to grain..It's 50-50 at best. If you want to do grain you have to go down the agar route, its easy, saves you wasting time, and actually fun so there's no reason not to

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OfflineNN-TNT
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Registered: 08/04/18
Posts: 15
Last seen: 4 years, 3 months
Re: g2g Bacterial blasphemy Exhausted morning post, don't mind me... [Re: Shroomspective]
    #25623621 - 11/18/18 04:27 PM (6 years, 1 month ago)

Sounds good, I'll get a new project going, I'm thinking brf agar that I strain would be easier than grain wash agar, so I'll try that out!

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