Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Pinkish Tint on Freshly Germinated Mycelium
    #25617264 - 11/15/18 07:31 PM (6 years, 2 months ago)

Hello,

I inoculated my jars on November 11th, and they germinated after 3 days, on the 14th.

Up until today all the growth was cottony white. Today though, I spotted some pinkish tint over some of them, and I fear that it might be some sort of contamination.

Here's a picture;

Now the camera didn't quite pick up the colors, but there's a light reddish tint on the white. What do you guys think? Is it contaminated?

Rhada

Edited by Rhada (11/16/18 04:55 AM)

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25617669 - 11/15/18 11:24 PM (6 years, 2 months ago)

Post the pics to the gallery, not external links:thumbup:

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25617896 - 11/16/18 04:59 AM (6 years, 2 months ago)

Done.

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25618552 - 11/16/18 10:56 AM (6 years, 2 months ago)

Here is another picture from today;

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25618569 - 11/16/18 11:05 AM (6 years, 2 months ago)

Hard to see in the picture due to the lighting, give it another few days..if its not pure white under a white LED then you've got a problem

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25618589 - 11/16/18 11:13 AM (6 years, 2 months ago)

Even if it's a mild 'infection', could the mycelium eventually fight it off? Or once it's there, it's over?

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25618659 - 11/16/18 11:36 AM (6 years, 2 months ago)

If its infected then you trash it, there's no half way..But give it a few days and re-check

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25618697 - 11/16/18 11:48 AM (6 years, 2 months ago)

That's fuckin done man. Fusarium, or some lookalike.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Mad Season]
    #25618710 - 11/16/18 11:52 AM (6 years, 2 months ago)

Sad. I will have to order another batch of spores then. Would anything good come from burying them outside?

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25618712 - 11/16/18 11:53 AM (6 years, 2 months ago)

Maybe? I mean you have nothing to loss if it doesn't do anything.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Mad Season]
    #25618787 - 11/16/18 12:22 PM (6 years, 2 months ago)

I will keep them to colonize further for a few days, then I will bury them. I have 6 other jars that I inoculated separately and they haven't germinated yet. But the fear has taken over me. What I did do wrong, I don't know. I used distilled bottled water to make a spore syringe, and I didn't boil it beforehand. Would that be the reason? That's the only thing I can think of, other than that I believe I followed every instruction for the sake of being sterile.

Not thick enough dry verm layer? Damn, not knowing is agonizing.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25619131 - 11/16/18 03:08 PM (6 years, 2 months ago)

Yeah boil water for sure if you're making your own syringe. Get into agar if you wanna just bypass syringes and have clean inoc all the time. Spores as well won't always be clean, but the fact there's so much contam in your jar, I'd bet it was already introduced in the water.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Mad Season]
    #25619206 - 11/16/18 03:47 PM (6 years, 2 months ago)

I just ordered another print, and this time I'm going with agar and grain. Gonna complete my research and get all the materials needed until it arrives, then I'll strike with full force. Thank you.

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625204 - 11/19/18 12:01 PM (6 years, 1 month ago)

Update:

You know what I did? Healthy mycelium was already spreading across the jars, and while not being so sterile, I just scooped them out and transferred into a newly made jar.

Fingers crossed.

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625249 - 11/19/18 12:24 PM (6 years, 1 month ago)

Seems unlikely to succeed, but it's all a learning experience

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25625259 - 11/19/18 12:32 PM (6 years, 1 month ago)

Yeah, I don't have high hopes either.

Fresh spores and agar supplies are on the way though, I'm counting on them.

One way or another I'm doing this.

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625295 - 11/19/18 12:51 PM (6 years, 1 month ago)

Good stuff..decent pressure cooker and PP5 pots\plates?

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25625323 - 11/19/18 01:01 PM (6 years, 1 month ago)

Pots for burying? I'll bury them outside. I have a SGFC, thinking of making a monotub also. (I will use trays for bulks)

I have a domestic pressure cooker, thinking that will suffice.

Edited by Rhada (11/19/18 01:04 PM)

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625347 - 11/19/18 01:09 PM (6 years, 1 month ago)

No PP5 pots for agar usage, or you'll need to buy some petri dishes..Long as your PC has decent pressure, ideally you want 15psi and enough space for quart jars to fit for grains

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25625434 - 11/19/18 01:51 PM (6 years, 1 month ago)

Ah, I've ordered some petri dishes and pre-made nutrient agar. I will sterilize them along with my grain jars and for lid air exchange I will use tyvek cut from children pencil box :P

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625455 - 11/19/18 02:04 PM (6 years, 1 month ago)

Sounds about right..don't forget to go out and get a big tote 66qt+ for a still air box, but flip it upside down and burn two arm holes in and then you're good to go

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25625462 - 11/19/18 02:07 PM (6 years, 1 month ago)

I made one with gloves attached. Though it's not that big and limits movement to some degree, it still does the job. I am planning on cutting gloves at my arms length to turn it into a SAB

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25625546 - 11/19/18 02:52 PM (6 years, 1 month ago)

Caution on the gloves..You can make a piston effect and actually drag more dust and spores through when you use them, compared to free arm holes

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25629219 - 11/21/18 05:11 AM (6 years, 1 month ago)

So far so good;

I punched a gas exchange hole in the lid and covered with double layer of micropore tape. No dry verm layer. All the new growth is snow white and I see no contaminations. If it colonizes to full like this, I am thinking of making a BRF to grain transfer.

Edited by Rhada (11/21/18 05:14 AM)

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25629981 - 11/21/18 01:14 PM (6 years, 1 month ago)

If it manages to get to 100%, but I'm not sure about transferring BRF to grains..I'd take some mycelium and make some agar personally as otherwise you'll probably end up wasting the grains when they contam out after a week or two

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25630103 - 11/21/18 02:00 PM (6 years, 1 month ago)

Hey, so do you suggest using the mycelium in the BRF to inoculate agar, instead of just starting over with the spores? if so, hell yeah, I'm down with that.

Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Rhada]
    #25630221 - 11/21/18 02:38 PM (6 years, 1 month ago)

Exactly:thumbup: Anything else is a gamble, whereas with agar you can physically see if spawn is clean..Take it to agar, once you see growth, transfer to a new plate..I usually get to T3 before I do anything, but sometimes use T2 if it's been a particularly good looking plate that's grown to the edges

Extras: Filter Print Post Top
OfflineRhada
Stranger
 User Gallery

Registered: 11/15/18
Posts: 23
Last seen: 5 years, 10 months
Re: Pinkish Tint on Freshly Germinated Mycelium [Re: Shroomspective]
    #25630347 - 11/21/18 03:23 PM (6 years, 1 month ago)

Ow yeah, I'm doing that. Thank you.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order


Similar ThreadsPosterViewsRepliesLast post
* Red Tint in jars a few days after inocculation? Ketaset 1,232 6 07/20/02 01:56 PM
by Aeolus1369
* Mycelium taking back substrate? dmc_illz 1,126 2 03/26/03 05:51 AM
by TheHateCamel
* confusing cobweb with mycelium? Snobrdr311 2,424 1 12/03/01 09:51 AM
by puscle
* Discolored mycelium Rabbitt 3,004 12 02/23/04 11:30 PM
by TheGanjaGorilla
* Yellow Mycelium Twitch 4,761 6 09/22/03 04:03 AM
by 40oz
* Slow mycelium action, two cheap small tricks and BOOM. blacksh33p18 1,902 15 10/30/04 04:58 AM
by blacksh33p18
* mycelium potency safeway 1,399 12 09/22/03 02:12 AM
by daussaulit
* can spores germinate in water? XOCHI 2,444 2 06/26/02 05:53 AM
by Mycelium

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,272 topic views. 29 members, 104 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.004 seconds on 12 queries.