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23harris23
Reptilian Overlord


Registered: 10/04/18
Posts: 57
Last seen: 5 years, 9 months
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Store bought agar for isolation
#25616092 - 11/15/18 10:17 AM (6 years, 2 months ago) |
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Is store bought agar used for food purposes sufficient for mushroom genetic isolation, or should I use "research grade" agar? Also are certain agar types more resistant to contamination?
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ithikuss
Level 4 Mage



Registered: 08/13/18
Posts: 650
Loc: Nightosphere
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Re: Store bought agar for isolation [Re: 23harris23]
#25616122 - 11/15/18 10:30 AM (6 years, 2 months ago) |
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making your own would be the best route, I imagine.
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23harris23
Reptilian Overlord


Registered: 10/04/18
Posts: 57
Last seen: 5 years, 9 months
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Re: Store bought agar for isolation [Re: ithikuss]
#25616357 - 11/15/18 12:13 PM (6 years, 2 months ago) |
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Quote:
ithikuss said: making your own would be the best route, I imagine.
If I was making my own agar what, if any nutrients would I need to add? Also would gelatin work as an agar substitute?
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Tormato  
Chairman of the Bored




Registered: 07/01/17
Posts: 6,106
Last seen: 2 days, 22 hours
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Re: Store bought agar for isolation [Re: 23harris23]
#25616369 - 11/15/18 12:20 PM (6 years, 2 months ago) |
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Quote:
23harris23 said: Is store bought agar used for food purposes sufficient for mushroom genetic isolation, or should I use "research grade" agar? Also are certain agar types more resistant to contamination?
You can use either grade of Agar and no, not that I'm aware of.
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teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 1 year, 5 months
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Re: Store bought agar for isolation [Re: 23harris23]
#25616371 - 11/15/18 12:21 PM (6 years, 2 months ago) |
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Don't isolate, just clean up.
Unless you are determined to get predictable, consistent results, MS cultures will serve you just well. Isolating requires you to test a lot of culture, and will take anything from 10-100 transfers.
Agar recipes are plentiful, use the menu bars or search engine.
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ithikuss
Level 4 Mage



Registered: 08/13/18
Posts: 650
Loc: Nightosphere
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Re: Store bought agar for isolation [Re: teladi]
#25616416 - 11/15/18 12:46 PM (6 years, 2 months ago) |
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Quote:
teladi said: Agar recipes are plentiful, use the menu bars or search engine.
 I've been doing light malt myself.. but there are a good amount of recipes to choose from.
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RalphieRamone
Illustrious Nemophilist



Registered: 08/12/18
Posts: 104
Loc: Jupiter
Last seen: 4 years, 4 months
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Re: Store bought agar for isolation [Re: ithikuss]
#25616819 - 11/15/18 04:15 PM (6 years, 2 months ago) |
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I cant wait to start getting into agar...it seems alot more manageable and easier then doing spore syringes or an LC.
If you've got sterile procedures down agar should be a piece of cake right?
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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There are different types of agar for cooking. Be sure that it's 100% agar, with no added sugar.
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