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Invisiblepezhvak
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Registered: 10/26/16
Posts: 60
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Agar mycelium growth question
    #25615631 - 11/15/18 03:39 AM (6 years, 2 months ago)

hi everybody!
this is my first time doing agar on petri dishes, here are two, the first one is blueoyster, the second one is golden teacher.



i have few questions:

1- since i have used pin/grain in my petri dishes do i still have sections to transfer to new dishes?

2- are they looking fine?

3- from where should i take samples and transfer to new dishes (in order to get clean culture)

4- is monoculture possible?

5- why my gt (the right one) grew uneven? should i transfer from leading edges?

sorry i know most of my questions are stupid, i'm new to agar. :confused:


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Know thyself, and thou shalt know the universe and God

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Invisibleelasticaltiger
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Re: Agar mycelium growth question [Re: pezhvak]
    #25615636 - 11/15/18 03:48 AM (6 years, 2 months ago)

1 - yes.
2 - yes.
3 - the leading edge of areas that look like clean or aggressive growth.
4 - yes
5 - this happens sometimes. Yes transfer from the lead edge of each of those offshoots into it's own dish.


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Invisiblepezhvak
Teacher
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Registered: 10/26/16
Posts: 60
Loc: Universe Kernel
Re: Agar mycelium growth question [Re: elasticaltiger]
    #25615654 - 11/15/18 04:09 AM (6 years, 2 months ago)

thank you for your fast reply, how do i identify different sections? any good link that i can learn or could you please tell me a bit so i can start researching based on that?

it's clear to me where i should take samples from GT dish, but i don't know about the blueoyster.


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Know thyself, and thou shalt know the universe and God

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Offlinedelcat
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Registered: 03/13/18
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Re: Agar mycelium growth question [Re: pezhvak]
    #25615799 - 11/15/18 06:58 AM (6 years, 2 months ago)

Blue oyster looks quite even to me.  If I were you, I'd just pick a few different spots to transfer almost at random.  As you've already stated, there isn't a lot of difference.  Decide how many samples you are going to take. Try and identify that many areas that have the most difference between them, and just take the samples from there.

If it were me I'd probably pick:

9pm - Its growing the fastest
11:30pm - It looks very thick
4pm - also growing fast and thick

Then I'd probably pick the worst looking section I can and sample from that (for some reason I always like to pick the worst looking non contaminated sector as well, I have been surprised by it in the past)

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OfflineBLINKfan420
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Re: Agar mycelium growth question [Re: delcat]
    #25615921 - 11/15/18 08:27 AM (6 years, 2 months ago)

Here ya go...



BLiNK


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All my pictures are drawn from imagination.  I don't even know what a mushroom is.

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