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OfflineSpicyTrapeze
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Registered: 11/12/18
Posts: 2
Last seen: 6 years, 29 days
cobweb in new mono?
    #25609121 - 11/12/18 10:31 AM (6 years, 2 months ago)



Third mono attempt with the same spawn run, fourth day.  Would cobweb grow this quickly from spores in four days on my casing layer?  Or is it that the whole spawn run was rubbish?  I'm starting from scratch from agar for a new set, but I'm also considering filling a mono with just coir to see if it's something in my air.  If it is my air, I'm not sure what to try next.  Between the second and third attempt I cleaned the whole space with vinegar and ozone.  If it is just cobweb from the air, should I spray with peroxide?  Could I dig into the tub to identify if the mold is growing off the spawn?

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OfflineSchizophriendly
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Registered: 10/26/18
Posts: 4
Last seen: 6 years, 1 month
Re: cobweb in new mono? [Re: SpicyTrapeze]
    #25610230 - 11/12/18 05:52 PM (6 years, 2 months ago)

Yeah, the middle left and right, dark-grayish growth spots has to be cobweb, or something similar. It's color contrast is too different from the other spots of mycelium growth.
It could have grown on the spawn but didn't completely defeat the mycelium, or a speck could have gotten onto the casing layer, with enough moisture, then just grew out on uncolonized space of the sub.
As for the casing, I think you might have used too much of something. The casing shouldn't have nutrients in it, enough to cause spores to grow on top of it. Or at least, it shouldn't be so thick that the mycelium has to try and colonize through it.
You might just want to toss it. Since it's partially visible, those healthy mycelium spots should have started to form knots by now or something. That contam has taken over.

I'm new here, so take my opinion with a grain of salt. If you try and save it with peroxide or sunlight exposure, just make sure you don't butcher it physically.

Also, does ozone spray clean bacteria from air by only physically or hydraulically pushing them, and trapping them to a surface as it settles in the air? That's how it blocks out odors and scents, right?


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Edited by Schizophriendly (11/12/18 05:53 PM)

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OfflineSpicyTrapeze
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Registered: 11/12/18
Posts: 2
Last seen: 6 years, 29 days
Re: cobweb in new mono? [Re: Schizophriendly]
    #25632567 - 11/22/18 01:29 PM (6 years, 1 month ago)

Update:

I managed the cobweb for a week by misting the bins with peroxide.  One has now gone to trich.  The upside is that the trich bin is also full of gnats, and I remember at least one of the first two bins also having gnats.  I have houseplants and can imagine that trich could survive ozone if it's deep enough in the soil, then the gnats must be spreading it everywhere again afterwards.  I've ordered some gnat traps and will also down a bottle of red wine today to get started on clearing out my gnat problem.  Depending on how i'm feeling after the wine I might also bin my houseplants.  I didn't realize how strongly the relationship between houseplant, trich, and gnats can work against mushroom cultivation.  The last bin is about 70% colonized I'd say.  No signs of trich or gnats yet, but i'm going to stare at it for a bit and if I see a gnat i might open it up and try to save it by blasting it with ozone.  The myc on the bottom should survive and I might get a fruit or two.

As an aside, while I'm excited to be learning so much through multiple failures, it is starting to get frustrating not having a single success and I suspect that minimally Bod's unmodded tub won't work in my conditions.  I might go back to PF for a bit and see if I can find more success that way while iterating on my LC/agar/isolate work.

What do you guys think about cutting the edge of rhizomatic mycelial growth from the edge of my trich bin to start new isolates?  While somewhat slow colonizers, I'd be interested in how they ultimately perform given they've already proven the ability to eat coir.

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InvisibleShroomspective
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Registered: 09/26/15
Posts: 1,725
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Re: cobweb in new mono? [Re: SpicyTrapeze]
    #25632651 - 11/22/18 02:08 PM (6 years, 1 month ago)

Trich and cobweb are usually symptoms of bad spawn, it was probably bacterial to start with. Clean spawn in a mono is pretty much resistant to contam for the first flush or two..So I wouldn't throw my house plants away, but you do need to control the gnats

Def avoid taking mycelium from your infected mono..Get some spore and some agar and germinate on them, then A2g and G2g

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