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Invisiblepezhvak
Teacher
Male


Registered: 10/26/16
Posts: 60
Loc: Universe Kernel
Re: Using Wheat to spawn (HELP) [Re: puff4200]
    #25602432 - 11/09/18 07:08 AM (6 years, 2 months ago)

yeah, i used spores to make my syringe, the first time it worked perfectly but i made my own spore prints that might be it! because the second syringe that i prepared was from my own print, alright, so now that i'm using agar, they will show up on my plate, and i should sample from those areas that are clean and move them to another dish until i get a clear culture is that right? is there any good tutorial for learning and recognizing different sections and how to do that the correct way?


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Know thyself, and thou shalt know the universe and God

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Invisiblepuff4200
Natural born lever puller

Registered: 09/26/10
Posts: 1,269
Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25602449 - 11/09/18 07:18 AM (6 years, 2 months ago)

Spores to grain is always luck. That's why caps said just work on a clean culture. I wouldn't worry about trying to get a monoculture (transferring until there is no sectors or focusing on specific sectors). Just put the spores on agar once they start growing make a few transfers and it should be clean. Don't transfer from any part of the plate that's green or filmy looking (like the hidden bacteria in the post I sent you).

Don't worry yourself about sectors and all that until you work from a clone. You won't get sectors from multispore unless you do shit tons of transfers. How to get a monoculture was covered pretty well in that post I sent you awhile ago.

Edited by puff4200 (11/09/18 07:33 AM)

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Using Wheat to spawn (HELP) [Re: puff4200]
    #25604808 - 11/10/18 08:01 AM (6 years, 2 months ago)

Could also be a result from prepping grain too wet...

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