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OfflineFroots
Stranger
Registered: 11/07/18
Posts: 8
Last seen: 6 years, 1 month
Excessive moisture in Easy Agar Tek, Contam or grain soak deposit?
    #25599828 - 11/07/18 11:27 PM (6 years, 2 months ago)

Long time lurker but finally kinda needed a question after not finding an answer from searching. I looked at the stickied contamination list and none mention much about what I am seeing.

Here are some Agar plates I transferred from Agar started with spores.
All of the first plates were Potato Flake and Karo Agar and turned out really clean regardless of the excessive moisture and coming from spore prints I took relatively carelessly.

All of the these plates (pictures) are Grain Soak and Karo Agar. I tried to take clean samples to transfer to new Grain Agar, EXCEPT for Plate 9 (tossed it).

This is 7 days of growth in a 78 degree chamber heated by a fish tank heater which keeps moisture pretty high. Other wise my apartment is pretty chilly.

Feedback on what the off white color is on bottom of plate 2, right side of plate 3, plate 5, 6, and all over 9.

Plate 1


Plate 2


Plate 3


Plate 4


Plate 5


Plate 6


Plate 7


Plate 8


Plate 9



Edited by Froots (11/13/18 09:23 PM)

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OfflineLassy123
Male


Registered: 12/22/17
Posts: 89
Last seen: 3 years, 4 months
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Froots]
    #25599850 - 11/07/18 11:58 PM (6 years, 2 months ago)

Possibly sediment from your grain soak water and agar powder? Perhaps not mixed well enough. That's all ive got buddy. :frown: Good news is you can just transfer it away if you're super paranoid.


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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 1 year, 5 months
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Lassy123]
    #25599872 - 11/08/18 12:29 AM (6 years, 2 months ago)

Is it changing at all? Was it there when you inoculated? Is it on the surface?

GSW does make a lot of sediment, unless you filter it.

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OfflineFroots
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Registered: 11/07/18
Posts: 8
Last seen: 6 years, 1 month
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: teladi]
    #25599893 - 11/08/18 12:57 AM (6 years, 2 months ago)

What it looks like is the moisture on the side of the containers ran down and pooled on the agar. I felt the cloudy "deposit" of plate 9 and it was slimy. It wasn't like that to begin with and I noticed it slowly grew.  I transferred to new plates and cut the "good" mycelium out and threw them into oat jars to see what happens.

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OfflineLassy123
Male


Registered: 12/22/17
Posts: 89
Last seen: 3 years, 4 months
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Froots]
    #25599902 - 11/08/18 01:24 AM (6 years, 2 months ago)

Pretty sure if you just transferred plate 8 to a quart of grain, see if you get clean spawn. Then Grain to grain that to 10 jars you'd be in business :thumbup:


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OfflineFroots
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Registered: 11/07/18
Posts: 8
Last seen: 6 years, 1 month
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Lassy123]
    #25599914 - 11/08/18 01:40 AM (6 years, 2 months ago)

I transferred all except 9 to grain and labeled which plates they came from. 8 looked the best and 4 wasn't too bad. Others look iffy

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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 1 year, 5 months
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Froots]
    #25599933 - 11/08/18 01:59 AM (6 years, 2 months ago)

Quote:

Froots said:
What it looks like is the moisture on the side of the containers ran down and pooled on the agar. I felt the cloudy "deposit" of plate 9 and it was slimy. It wasn't like that to begin with and I noticed it slowly grew.  I transferred to new plates and cut the "good" mycelium out and threw them into oat jars to see what happens.




That's bacteria. Try keep your containers upside down to prevent condensation from falling onto your agar. Often condensation picks up contaminants, and can deposit them onto your medium.

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OfflineFroots
Stranger
Registered: 11/07/18
Posts: 8
Last seen: 6 years, 1 month
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: teladi]
    #25599937 - 11/08/18 02:06 AM (6 years, 2 months ago)

Quote:

teladi said:
Quote:

Froots said:
What it looks like is the moisture on the side of the containers ran down and pooled on the agar. I felt the cloudy "deposit" of plate 9 and it was slimy. It wasn't like that to begin with and I noticed it slowly grew.  I transferred to new plates and cut the "good" mycelium out and threw them into oat jars to see what happens.




That's bacteria. Try keep your containers upside down to prevent condensation from falling onto your agar. Often condensation picks up contaminants, and can deposit them onto your medium.




Is it likely that all of the mycelium that was in the plates with the bacteria is contaminated? Or will I be able to transfer and isolate clean material?

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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 1 year, 5 months
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: Froots]
    #25600005 - 11/08/18 04:03 AM (6 years, 2 months ago)

Keep transferring till clean. The contam may be from any number of sources.

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OfflineFroots
Stranger
Registered: 11/07/18
Posts: 8
Last seen: 6 years, 1 month
Re: Excessive moisture in Easy Agar Tek, Contam or grain soak deposit? [Re: teladi]
    #25612895 - 11/13/18 07:38 PM (6 years, 2 months ago)

UPDATE.

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