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OfflineAkashaPrinciple99
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Liquid Culture need hole in lid?
    #25592918 - 11/05/18 01:03 AM (6 years, 2 months ago)

If im going to be making a honey and water LC, do I need to have a hole in the lid for FAE or can it be sealed?

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OfflineWhiteVeil
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Re: Liquid Culture need hole in lid? [Re: AkashaPrinciple99]
    #25592922 - 11/05/18 01:10 AM (6 years, 2 months ago)

I don't have much experience with LC but I thought you needed to have a hole in the lid for later, as in putting the needle through so you can collect LC in your syringe.

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OfflineAkashaPrinciple99
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Re: Liquid Culture need hole in lid? [Re: WhiteVeil]
    #25592923 - 11/05/18 01:11 AM (6 years, 2 months ago)

Quote:

WhiteVeil said:
I don't have much experience with LC but I thought you needed to have a hole in the lid for later, as in putting the needle through so you can collect LC in your syringe.





I am not innoculating using a spore syringe, going to be cloning with pieces cut from the inside of a stem.

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Offlinel0wbob
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Re: Liquid Culture need hole in lid? [Re: AkashaPrinciple99]
    #25592940 - 11/05/18 01:25 AM (6 years, 2 months ago)

You should first get into agar if you want to make LC.

Also you can only tell if a LC is contaminated or not by testing it on agar. ( or wasting prepared grain )


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OfflineAkashaPrinciple99
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Re: Liquid Culture need hole in lid? [Re: l0wbob]
    #25592947 - 11/05/18 01:32 AM (6 years, 2 months ago)

Quote:

l0wbob said:
You should first get into agar if you want to make LC.

Also you can only tell if a LC is contaminated or not by testing it on agar. ( or wasting prepared grain )





All good, ive done this TEK before years ago and it worked, just trying to refresh my memory on the hole in the lid.

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Offlinel0wbob
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Re: Liquid Culture need hole in lid? [Re: AkashaPrinciple99]
    #25592963 - 11/05/18 01:48 AM (6 years, 2 months ago)

yep you need some FAE but i wouldn't do it with polyfill or micropore.

i prefer 0.22ยตm filters


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OfflineAkashaPrinciple99
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Re: Liquid Culture need hole in lid? [Re: l0wbob]
    #25592966 - 11/05/18 01:57 AM (6 years, 2 months ago)

Quote:

l0wbob said:
yep you need some FAE but i wouldn't do it with polyfill or micropore.

i prefer 0.22ยตm filters




No worries, thanks!

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Invisible36fuckin5
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Re: Liquid Culture need hole in lid? [Re: AkashaPrinciple99]
    #25593484 - 11/05/18 09:31 AM (6 years, 2 months ago)

Quote:

AkashaPrinciple99 said:
Quote:

l0wbob said:
You should first get into agar if you want to make LC.

Also you can only tell if a LC is contaminated or not by testing it on agar. ( or wasting prepared grain )





All good, ive done this TEK before years ago and it worked, just trying to refresh my memory on the hole in the lid.




It's not a reliable method. You got lucky. Use agar.


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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlineteladi
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Re: Liquid Culture need hole in lid? [Re: 36fuckin5]
    #25593530 - 11/05/18 09:50 AM (6 years, 2 months ago)

You can do it with any filter, but make sure you don't get them wet when shaking. Polyfill will pass through contaminants if it is wet by your LC.

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Invisiblestareatclouds
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Re: Liquid Culture need hole in lid? [Re: l0wbob] * 1
    #25593603 - 11/05/18 10:28 AM (6 years, 2 months ago)

Quote:

l0wbob said:
yep you need some FAE but i wouldn't do it with polyfill or micropore.

i prefer 0.22ยตm filters




No you don't. FAE has nothing to do with this. It'd be for GE, but the primary reason is so you don't pull a vacuum.

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