Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | < Back | 987 | 988 | 989 | 990 | 991 | 992 | 993 | 994 | 995 | 996 | 997 | 998 | 999 | 1000 | 1001 | 1002 | 1003 | 1004 | 1005 | 1006 | 1007 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Bumholio]
    #25588236 - 11/03/18 02:31 AM (5 years, 3 months ago)

doesnt look like mold to me just keep an eye on it


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
InvisibleBumholio
What's the craic
 User Gallery


Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25588249 - 11/03/18 03:02 AM (5 years, 3 months ago)

10/4


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

Extras: Filter Print Post Top
OfflineCrispykoot
Jello Wrangler
Male User Gallery


Registered: 10/16/16
Posts: 5,922
Loc: Flag
Last seen: 3 days, 23 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Bumholio]
    #25588432 - 11/03/18 06:51 AM (5 years, 3 months ago)

Looking for opinions from people who do liquid inoculation in front of a flow hood.

Is it reasonable to leave the donor jar with the liquid innoculant open with no lid on while doing the innoculations or should the lid go back on in between jars? 

I leave mine open in front of the hood and do 18 jars with it. Is that too risky? I have a relatively new 2x4 foot FP HEPA filter with a pre filter. I'm trying to run down the source of bacteria in some of my cultures.  Thanks in advance.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery

Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: Crispykoot]
    #25588440 - 11/03/18 06:56 AM (5 years, 3 months ago)

When i use liquids I just keep it open. One reason being I don't have a good place to put the lid.

Since it's new how's your other work agar? Could not be getting enough out of your fh. Just a thought since it's new.


If you keep it open take care when wiping down the next set of jars. I'm always paranoid that somehow something will fly off into whatever I have open.

Maybe it's hidden in your lc. Do you use regular mouth? how do you knock them?


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery

Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: Crispykoot]
    #25588443 - 11/03/18 06:58 AM (5 years, 3 months ago)

Quote:

Crispykoot said:
I'm trying to run down the source of bacteria in some of my cultures.





Some is key word here. It's probably your cultures


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
OfflineCrispykoot
Jello Wrangler
Male User Gallery


Registered: 10/16/16
Posts: 5,922
Loc: Flag
Last seen: 3 days, 23 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: van hatton]
    #25588534 - 11/03/18 08:18 AM (5 years, 3 months ago)

It's probably not the cultures as I use antibiotic agar. They look beautiful on plates. I've run down all the other vectors that I can think of and feel good about that part. There's just this last question I'm trying to pin down.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

Extras: Filter Print Post Top
Invisibleforagedfungus
 User Gallery


Registered: 09/30/13
Posts: 1,851
Loc: out there
Re: Cultivation General Discussion [Re: Crispykoot]
    #25588560 - 11/03/18 08:45 AM (5 years, 3 months ago)

Maybe try running them on regular MEA plates for the last transfer before liquid?
The antibiotic may be preventing bacteria from growing vigorously enough to see, but it could still be riding along?

Extras: Filter Print Post Top
Invisiblevan hattonFacebookDiscord
Still a noob
 User Gallery

Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: Cultivation General Discussion [Re: foragedfungus]
    #25588613 - 11/03/18 09:25 AM (5 years, 3 months ago)

Is this like with everything or just some things?


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Offlinebutterflyaway
Kaleidoscope Queen
I'm a teapot User Gallery


Registered: 10/26/18
Posts: 114
Loc: Flag
Last seen: 3 years, 6 months
Re: Cultivation General Discussion [Re: Mateja]
    #25588866 - 11/03/18 11:21 AM (5 years, 3 months ago)

What should I do with the tiny pins?  How will I know it's time for the second flush?  & should expect anything different from the second flush?


--------------------
There is a better way.  Finding the way — well that's the journey:mushroom2:

Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: foragedfungus] * 1
    #25589144 - 11/03/18 01:48 PM (5 years, 3 months ago)

Quote:

Crispykoot said:
It's probably not the cultures as I use antibiotic agar. They look beautiful on plates. I've run down all the other vectors that I can think of and feel good about that part. There's just this last question I'm trying to pin down.



Quote:

foragedfungus said:
Maybe try running them on regular MEA plates for the last transfer before liquid?
The antibiotic may be preventing bacteria from growing vigorously enough to see, but it could still be riding along?



:whathesaid: antibiotic agar has it's place, but I feel like it hides shitty technique. I like to use agar because I can KNOW if there's something in my culture. if bacteria can't grow how do I know if it's there? 

take everything I say with a grain of salt. but that's :2cents:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25589194 - 11/03/18 02:14 PM (5 years, 3 months ago)

Do any of you have a preferential technique when doing a2a? I realized recently that the triangle cut I do only has a small portion of the leading edge and a larger chunk of the already colonized agar.

Gonna try flipping it the other way, or I'm open to other suggestions on good ways to make the cuts and transfers.

Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Cultivation General Discussion [Re: MooseShroom] * 1
    #25589203 - 11/03/18 02:16 PM (5 years, 3 months ago)

Just make sure your hand doesn’t hang over the plate. I usually hold the plate sideways and take my transfer(s).
And always use lots of alcohol lol.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery

Extras: Filter Print Post Top
OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
Re: Cultivation General Discussion [Re: Buck513]
    #25589211 - 11/03/18 02:19 PM (5 years, 3 months ago)

Quote:

Buck513 said:
Just make sure your hand doesn’t hang over the plate. I usually hold the plate sideways and take my transfer(s).
And always use a bunch of alcohol lol.




Yeah, I always have my plates start out upside down (use plates similar to Josex's) and have them at an angle when I make the cut. Actively try not to put my hand over the plate. My technique I don't feel is sloppy, but always room to improve :thumbup:

Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: MooseShroom] * 1
    #25589388 - 11/03/18 03:40 PM (5 years, 3 months ago)

I find it best to make the angle going away from the center like so


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25589434 - 11/03/18 04:01 PM (5 years, 3 months ago)

Quote:

tryptkaloids said:
I find it best to make the angle going away from the center like so




I'll give that way a shot. The transfers I had been taking were like the blue triangle in this photo

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 3 months
Re: Cultivation General Discussion [Re: MooseShroom] * 1
    #25589503 - 11/03/18 04:22 PM (5 years, 3 months ago)

@tryptkaloids

Antibacterial agar (intended to limited bacteria activity) > Normal agar (to validate there is no bacteria).

Potentials: Germinate mycelium without germinating bacteria | Comparison: You germinate both.

:2cents:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 26 days
Re: Cultivation General Discussion [Re: stareatclouds]
    #25589554 - 11/03/18 04:41 PM (5 years, 3 months ago)

Quote:

stareatclouds said:
I made it about 9 seconds until the dude called it a "labtop." I fail to see how putting a phone in air-tight Tupperware, separated from a desiccant/rice would be an issue. But I'm not watching 16 minutes to figure it out either.



Lol I'm not trying to shit on you or anything but if you watched further you would hear him say "laptop or phone". You were a second or two too short on the patience.

Didn't expect you to watch whole video anyway. By the time phone stops working it's probably already corroded shorted and messed up. It could help if you immediately stick it into rice but I'd argue you would be better off putting it into oven with a fan on low temperature like 50 degrees centigrade.
Quote:

Ferather said:
@tryptkaloids

Antibacterial agar (intended to limited bacteria activity) > Normal agar (to validate there is no bacteria).

Potentials: Germinate mycelium without germinating bacteria | Comparison: You germinate both.

:2cents:



Is antibacterial agar worth it?


--------------------
One day I will evolve from agar mushroom grower :goat:

Edited by IntergalacticSpore (11/03/18 04:44 PM)

Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25589580 - 11/03/18 04:53 PM (5 years, 3 months ago)

that's why I said it has it's place, for dirty spores or a stubborn clone antibiotics can be amazing. for every day transfers I like to know if I'm letting anything in. how will I see bacterial satelites if they can't show up?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 3 months
Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25589581 - 11/03/18 04:53 PM (5 years, 3 months ago)

@IntergalacticSpore

I wouldn't buy it, very expensive stuff. I use another method (T-Gel), validated to work by various members, seriously cheaper.

----

@tryptkaloids

Put it on normal agar (after), the recipe you would normally use to spot bacteria, then make spawn.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
InvisibleBumholio
What's the craic
 User Gallery


Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: Cultivation General Discussion [Re: Ferather]
    #25589617 - 11/03/18 05:09 PM (5 years, 3 months ago)

Quote:

Ferather said:
I use another method (T-Gel),




Would there be any benefit to using tea during the grain soak?


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 987 | 988 | 989 | 990 | 991 | 992 | 993 | 994 | 995 | 996 | 997 | 998 | 999 | 1000 | 1001 | 1002 | 1003 | 1004 | 1005 | 1006 | 1007 | Next > | Last >

Shop: MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* question about outdoor cultivation WhoWhatWhen 1,842 11 04/16/03 12:36 AM
by oO_wombat_Oo
* Supa’s LIVE/UNCUT H2O2 experiments!
( 1 2 all )
SupaThaRipper 545 39 02/29/24 05:43 PM
by SupaThaRipper
* Post Your Cultivation Picture of The Day
( 1 2 3 4 ... 7479 7480 )
saralove 4,322,382 149,581 03/01/24 02:33 AM
by Clued Up Shroomer
* Start to Finish - Complete Guide to Bulk Cultivation erags 12,471 11 03/25/05 11:15 PM
by Riggidy
* Hydrogen Peroxide (h2o2) often propagated mistruths lysergic 6,626 11 07/13/03 04:45 PM
by Baby_Hitler
* alt.drugs.mushrooms.cultivation (good/bad idea) BikeCourier 6,462 9 10/26/21 06:04 PM
by LogicaL Chaos
* Vegetative growth VS generative growth? LoveLightPeace 1,546 1 06/04/18 02:15 PM
by jason9086
* Re: 101 ways to use h2o2? Anonymous 1,363 7 02/14/00 12:27 AM
by toad

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,199,187 topic views. 20 members, 214 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.046 seconds spending 0.024 seconds on 14 queries.