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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 11 months
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Mycelium halting colonization, no visible contam.
#25588459 - 11/03/18 07:14 AM (6 years, 2 months ago) |
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27th oct (left one)
1st nov
3rd nov
I have 8 jars who had the same issue and 2 jars from the previous run which are about 3 weeks old now. All of them get dropped and "mycelium" relatively quickly expands, doesnt look like normal mycelium though, then after it exhausts nutrition and moisture in agar I dropped inside (I shook up a few and saw agar completely dried out). It stops expanding. There have been no contamination in the jars and the grains certainly have enough moisture as I only strained them for 5-10 minutes, cooked rye grains for 12 minutes, on previous run of 2 jars I had grains only on this run I had 1 cup of coffee, 1/2 cup of brewers yeast 1/2 cup gypsum in 1500g dry rye grain. I dried them with a towel on previous run so they are way drier. Both methods result in the same thing.
There is no visible contamination no water puddle on bottom of the jar and darkness to light makes no difference. The jars are on 22C/72F and nothing seems to be happening.
What could be going on with my mushrooms. What kind of infection could this be if it is infection. The agar I used for the last batch was taken out of 4 petris with the nicest uniform non rhizo growth, which didnt look bacterial and had no mold infections and after I waited for it to fully colonize it reached the edge of my rye flour/brewers yeast/agar substrate on every side and started climbing up on the walls.
The agar for inoculation was 2nd tier for previous run and 3rd tier agar that started from clones which were taken from a 100% mycelium grow kit I started this hobby with (1st tier) that later after fully colonizing turned into this on 4/7 jars (3 were contaminated and discarded).
 This is first tier agar later on, the other 3 had 3-4 fruits each the reason why they fruited is I used 7% nutrition in the first tier agar, later on I used 2-3%.
I'm really lost and currently waiting for my order for spore syringe to go through because at this point I no longer have a living culture to collect spores from and after 2 months and 20 tries I've started to blame genetics since contams aren't visible in any way to me and substrate can't be the reason.
Im not giving up on this stuff because I like doing it but what is going on here? The strain is mckennai I can disclose the company that I bought the 100% mycelium growbox from but I'm trying to respect forums rules about not advertising non sponsors.
-------------------- One day I will evolve from agar mushroom grower
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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How old is the culture? Might be senescence if the culture was old.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 11 months
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Re: Mycelium halting colonization, no visible contam. [Re: Pastywhyte]
#25588901 - 11/03/18 11:40 AM (6 years, 2 months ago) |
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Quote:
Pastywhyte said: How old is the culture? Might be senescence if the culture was old.
I have no idea how old it is, I cloned it once on agar, the 100% mycelium box was bought from a well established website that is based in netherlands.
I bought it because I wanted to get cheaper shrooms, have had no prior knowledge or background in mycology. Only thought after seeing the thing "hey maybe I can do it myself" and started reading about it, registered on this site after the second flush was harvested. I will be getting spore syringe from one of our sponsors soon so if it's senescence that should fix it.
I'm waiting for it to start colonizing my dry malt extract agar, the honey agar looks really weird.
-------------------- One day I will evolve from agar mushroom grower
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zoomfan
doubt 'er


Registered: 07/16/09
Posts: 505
Loc: eastern Canada
Last seen: 3 years, 9 months
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First off if it's probably at its limit of senescence when they send it just makes financial sense for them to expand it as much as possible...I've had very similar problems with rye i almost wondered if it was treated with an antifungal growth was so hit or miss. also seem to have trouble getting moisture right with it.
I have a lot more success with wbs or millet and cracked corn. i've also had more success with jars that have visible water than ones that are dry, best to err on that side imo. your jars look fairly dry, they should look like a place mold would grow. i'm sure youre not a novice and have had plenty of success yourself, just my two cents.
-------------------- Thinking is dreaming wake up and enjoy the dream.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Re: Mycelium halting colonization, no visible contam. [Re: zoomfan] 1
#25589109 - 11/03/18 01:24 PM (6 years, 2 months ago) |
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Dry I bet if u opened one of those jars and tried to smash a grain it would be hard on the inside.
Switch to wheat Fuck rye. I had a bad experience with rye. Fucked my shit up for a year.
Plate pics
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 11 months
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Re: Mycelium halting colonization, no visible contam. [Re: van hatton]
#25589242 - 11/03/18 02:33 PM (6 years, 2 months ago) |
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Quote:
zoomfan said: First off if it's probably at its limit of senescence when they send it just makes financial sense for them to expand it as much as possible...I've had very similar problems with rye i almost wondered if it was treated with an antifungal growth was so hit or miss. also seem to have trouble getting moisture right with it.
I have a lot more success with wbs or millet and cracked corn. i've also had more success with jars that have visible water than ones that are dry, best to err on that side imo. your jars look fairly dry, they should look like a place mold would grow. i'm sure youre not a novice and have had plenty of success yourself, just my two cents.
Oh my god. So I'm not going crazy! Ive had the jars that have visible water have success as well! Also considered rye is treated but I got it from bio farmer so that can't be it. Also the same rye is getting colonized, although hella slow in these two jars, yeah I know they are bacterial, there is almost no doubt about it I can see the goo looking spots on the glass where the mycelium didn't colonize.
And here a picture from almost exactly a month ago.

Quote:
van hatton said: Dry I bet if u opened one of those jars and tried to smash a grain it would be hard on the inside.
Switch to wheat Fuck rye. I had a bad experience with rye. Fucked my shit up for a year.
Plate pics 
I put a jar on 26C and the walls of the jar got HEAVY water condensation on it, the rye grains are very moist on the inside. The jars were wet as hell the first day, the grains just pulled the moisture inside.
Anyways I'm going to write off these problems as senescence.
Here is the pictures van hatton, your agar pouring advice reward .
 Thanks guys. If anyone else has ideas what else it could be you can still drop your ideas. You have no idea how frustrating it was racking my brains at what the hell this could be sterilizing 3 jars at a time 3 hours of waiting for each just to watch it do 7 milimeters and then do nothing. If they all got infected (some jars got mold and I threw them out) I could easily blame my technique and my lack of attention to detail but when those jars didn't even get contams . I've been wondering if its bacterial but then it wouldn't go speedy gonzales on agar fuel and then take a nap. The grain most certainly wasnt contaminated prior to inoculation, I had it in pressure cooker for 15-20 min to heat up 2 hours at 121C and then 30 minutes until I could open without releasing pressure.
Edit: van hatton I hope you don't mean actual agar pics lol, I have no active agar cultures they are all fully colonized. Theres some first and second tier agar anyway, the denser white is the agar that bore fruits later haha
 You can see some pins already.
-------------------- One day I will evolve from agar mushroom grower
Edited by IntergalacticSpore (11/03/18 02:56 PM)
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 11 months
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 This is todays picture, the honey agar didn't kill anything. I just had no contams and the genes are so weak that when introduced to a new substrate it does some weird half translucent thing. I'd think there is no mycelium, just contams but the shape and growth speed matches.
Can a contam look like that? I got the A2A source from 3 different petri dishes from tier 3, these are tier 4 on the brown and tier 5 on green, the recipes are 2% honey brown and 2% light dry malt extract on green.
The right most jar is the same as the 3 above.
The backside picture is dry malt. The little petri dish agar pins are my second flush on agar. I'm unironically doing multiple flushes on agar, it's one of those 4/7 .
-------------------- One day I will evolve from agar mushroom grower
Edited by IntergalacticSpore (11/05/18 02:50 PM)
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