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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
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Re: Cultivation General Discussion [Re: Bumholio]
#25588236 - 11/03/18 02:31 AM (5 years, 3 months ago) |
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doesnt look like mold to me just keep an eye on it
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25588249 - 11/03/18 03:02 AM (5 years, 3 months ago) |
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10/4
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 "great things may come to those who wait, but only what's left by those who hustle"
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,922
Loc:
Last seen: 3 days, 23 hours
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Re: Cultivation General Discussion [Re: Bumholio]
#25588432 - 11/03/18 06:51 AM (5 years, 3 months ago) |
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Looking for opinions from people who do liquid inoculation in front of a flow hood.
Is it reasonable to leave the donor jar with the liquid innoculant open with no lid on while doing the innoculations or should the lid go back on in between jars?
I leave mine open in front of the hood and do 18 jars with it. Is that too risky? I have a relatively new 2x4 foot FP HEPA filter with a pre filter. I'm trying to run down the source of bacteria in some of my cultures. Thanks in advance.
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Shadowboxing the apocalypse and wandering the land
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Crispykoot]
#25588440 - 11/03/18 06:56 AM (5 years, 3 months ago) |
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When i use liquids I just keep it open. One reason being I don't have a good place to put the lid.
Since it's new how's your other work agar? Could not be getting enough out of your fh. Just a thought since it's new.
If you keep it open take care when wiping down the next set of jars. I'm always paranoid that somehow something will fly off into whatever I have open.
Maybe it's hidden in your lc. Do you use regular mouth? how do you knock them?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Crispykoot]
#25588443 - 11/03/18 06:58 AM (5 years, 3 months ago) |
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Quote:
Crispykoot said: I'm trying to run down the source of bacteria in some of my cultures.
Some is key word here. It's probably your cultures
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,922
Loc:
Last seen: 3 days, 23 hours
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Re: Cultivation General Discussion [Re: van hatton]
#25588534 - 11/03/18 08:18 AM (5 years, 3 months ago) |
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It's probably not the cultures as I use antibiotic agar. They look beautiful on plates. I've run down all the other vectors that I can think of and feel good about that part. There's just this last question I'm trying to pin down.
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Shadowboxing the apocalypse and wandering the land
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foragedfungus



Registered: 09/30/13
Posts: 1,851
Loc: out there
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Re: Cultivation General Discussion [Re: Crispykoot]
#25588560 - 11/03/18 08:45 AM (5 years, 3 months ago) |
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Maybe try running them on regular MEA plates for the last transfer before liquid? The antibiotic may be preventing bacteria from growing vigorously enough to see, but it could still be riding along?
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Is this like with everything or just some things?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Mateja]
#25588866 - 11/03/18 11:21 AM (5 years, 3 months ago) |
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What should I do with the tiny pins? How will I know it's time for the second flush? & should expect anything different from the second flush?
-------------------- There is a better way. Finding the way — well that's the journey
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
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Re: Cultivation General Discussion [Re: foragedfungus] 1
#25589144 - 11/03/18 01:48 PM (5 years, 3 months ago) |
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Quote:
Crispykoot said: It's probably not the cultures as I use antibiotic agar. They look beautiful on plates. I've run down all the other vectors that I can think of and feel good about that part. There's just this last question I'm trying to pin down.
Quote:
foragedfungus said: Maybe try running them on regular MEA plates for the last transfer before liquid? The antibiotic may be preventing bacteria from growing vigorously enough to see, but it could still be riding along?
antibiotic agar has it's place, but I feel like it hides shitty technique. I like to use agar because I can KNOW if there's something in my culture. if bacteria can't grow how do I know if it's there?
take everything I say with a grain of salt. but that's
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25589194 - 11/03/18 02:14 PM (5 years, 3 months ago) |
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Do any of you have a preferential technique when doing a2a? I realized recently that the triangle cut I do only has a small portion of the leading edge and a larger chunk of the already colonized agar.
Gonna try flipping it the other way, or I'm open to other suggestions on good ways to make the cuts and transfers.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Cultivation General Discussion [Re: MooseShroom] 1
#25589203 - 11/03/18 02:16 PM (5 years, 3 months ago) |
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Just make sure your hand doesn’t hang over the plate. I usually hold the plate sideways and take my transfer(s). And always use lots of alcohol lol.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: Buck513]
#25589211 - 11/03/18 02:19 PM (5 years, 3 months ago) |
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Quote:
Buck513 said: Just make sure your hand doesn’t hang over the plate. I usually hold the plate sideways and take my transfer(s). And always use a bunch of alcohol lol.
Yeah, I always have my plates start out upside down (use plates similar to Josex's) and have them at an angle when I make the cut. Actively try not to put my hand over the plate. My technique I don't feel is sloppy, but always room to improve
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
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Re: Cultivation General Discussion [Re: MooseShroom] 1
#25589388 - 11/03/18 03:40 PM (5 years, 3 months ago) |
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I find it best to make the angle going away from the center like so
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 8 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25589434 - 11/03/18 04:01 PM (5 years, 3 months ago) |
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Quote:
tryptkaloids said: I find it best to make the angle going away from the center like so 
I'll give that way a shot. The transfers I had been taking were like the blue triangle in this photo
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: MooseShroom] 1
#25589503 - 11/03/18 04:22 PM (5 years, 3 months ago) |
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@tryptkaloids
Antibacterial agar (intended to limited bacteria activity) > Normal agar (to validate there is no bacteria).
Potentials: Germinate mycelium without germinating bacteria | Comparison: You germinate both.
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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 3 years, 26 days
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Quote:
stareatclouds said: I made it about 9 seconds until the dude called it a "labtop." I fail to see how putting a phone in air-tight Tupperware, separated from a desiccant/rice would be an issue. But I'm not watching 16 minutes to figure it out either.
Lol I'm not trying to shit on you or anything but if you watched further you would hear him say "laptop or phone". You were a second or two too short on the patience.
Didn't expect you to watch whole video anyway. By the time phone stops working it's probably already corroded shorted and messed up. It could help if you immediately stick it into rice but I'd argue you would be better off putting it into oven with a fan on low temperature like 50 degrees centigrade.
Quote:
Ferather said: @tryptkaloids
Antibacterial agar (intended to limited bacteria activity) > Normal agar (to validate there is no bacteria).
Potentials: Germinate mycelium without germinating bacteria | Comparison: You germinate both.

Is antibacterial agar worth it?
-------------------- One day I will evolve from agar mushroom grower
Edited by IntergalacticSpore (11/03/18 04:44 PM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 18 minutes
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that's why I said it has it's place, for dirty spores or a stubborn clone antibiotics can be amazing. for every day transfers I like to know if I'm letting anything in. how will I see bacterial satelites if they can't show up?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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@IntergalacticSpore
I wouldn't buy it, very expensive stuff. I use another method (T-Gel), validated to work by various members, seriously cheaper.
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@tryptkaloids
Put it on normal agar (after), the recipe you would normally use to spot bacteria, then make spawn.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: Ferather]
#25589617 - 11/03/18 05:09 PM (5 years, 3 months ago) |
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Quote:
Ferather said: I use another method (T-Gel),
Would there be any benefit to using tea during the grain soak?
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 "great things may come to those who wait, but only what's left by those who hustle"
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