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OfflineTacoGod
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question about monoculture and cloning
    #25587224 - 11/02/18 04:09 PM (6 years, 2 months ago)

whats the difference between these two? how long can you keep a monoculture going in terms of transfers? would you have to constantly be doing G2G transfers? is it worth it to use a monoculture over a clone?

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OfflineVroomerMcZoomers
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Re: question about monoculture and cloning [Re: TacoGod] * 1
    #25587508 - 11/02/18 06:02 PM (6 years, 2 months ago)

A monoculture is a single individual. 

Mushrooms complete their sexual reproductive cycle after the spores germinate, it takes two germinated spores to make a new individual.  Analogous to sperm and egg coming together to make a new individual.

What develops out of germinated spores is hundreds of individuals, all woven together in that
mass of white. Isolating samples several times can lead to a monoculture. 

Cloning is what we call it when you take a little piece of a mushroom and grow it out. 

Believe it or not, even this likely is not an individual monoculture!  There are others in there too!

As for how many transfers you could do before it goes bad, it goes by sheer number of cell divisions mostly.  If you were trying to do it g2g, you'd kill it fast because it takes a lot of cell divisions to fill up a jar.

You have to keep a young sample on agar and take from it for grain and grain masters, once that sample starts to get used up, you have to grow out more plates.  That way you keep your source mycelium as young as possible.


--------------------
 

Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

Edited by VroomerMcZoomers (11/02/18 06:07 PM)

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OfflineTacoGod
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Re: question about monoculture and cloning [Re: VroomerMcZoomers]
    #25587661 - 11/02/18 07:18 PM (6 years, 2 months ago)

so do you suggest that if i want to keep a monoculture alive i should transfer it onto another petri dish?

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OfflineVroomerMcZoomers
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Re: question about monoculture and cloning [Re: TacoGod] * 1
    #25587776 - 11/02/18 08:08 PM (6 years, 2 months ago)

Yes. 

Do it as required to keep yourself supplied with the inoculant you need for grain jars and grain masters, as that mycelium will have undergone the least amount of cell divisions as practically possible.


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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OfflineTacoGod
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Re: question about monoculture and cloning [Re: VroomerMcZoomers]
    #25587790 - 11/02/18 08:14 PM (6 years, 2 months ago)

thanks alot! i appreciate it :smile:

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OfflineVroomerMcZoomers
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Re: question about monoculture and cloning [Re: TacoGod] * 1
    #25587810 - 11/02/18 08:22 PM (6 years, 2 months ago)

You're supposed to change the nutrient in your agar every time too when you're doing that.  It is said it helps keep it feeling young.


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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Invisiblepuff4200
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Re: question about monoculture and cloning [Re: VroomerMcZoomers] * 1
    #25587827 - 11/02/18 08:33 PM (6 years, 2 months ago)

It's also worth looking into making a slant if your trying to keep a culture long term. Make the slant take a price from it to make a petri. Work with that petri for awhile and when its genetics weaken you go back to the slant you have stored in the fridge. Slants last a couple years in the fridge.

https://www.shroomery.org/forums/showflat.php/Number/25018245/fpart/all/vc/1

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OfflineTacoGod
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Re: question about monoculture and cloning [Re: puff4200]
    #25587843 - 11/02/18 08:43 PM (6 years, 2 months ago)

thank you both :smile: I did a little more digging on how to store/keep a monoculture and found this
https://www.shroomery.org/forums/showflat.php/Number/5845947

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Invisiblestareatclouds
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Re: question about monoculture and cloning [Re: VroomerMcZoomers]
    #25588102 - 11/03/18 12:22 AM (6 years, 2 months ago)

Quote:

VroomerMcZoomers said:
You're supposed to change the nutrient in your agar every time too when you're doing that.  It is said it helps keep it feeling young.




You do not need to change the media every time you take a transfer.

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