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Offlinedrunkymonkey
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slowed down to nothing inoculation?
    #25585478 - 11/01/18 08:34 PM (6 years, 2 months ago)

i've have these for a while and been meaning to post this but yeah life.... anyway heres picture.. started strong and just stopped. i'm not sure why. Any advice or pointing to any material i could read appreciated.


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OfflineTormatoMFacebookDiscordReddit
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585485 - 11/01/18 08:37 PM (6 years, 2 months ago)

Quote:

drunkymonkey said:
i've have these for a while and been meaning to post this but yeah life.... anyway heres picture.. started strong and just stopped. i'm not sure why. Any advice or pointing to any material i could read appreciated.





Looks contaminated....give your jars a good shake and break up the myc real good and see if it recovers.


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InvisibleXnMe
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Re: slowed down to nothing inoculation? [Re: Tormato]
    #25585537 - 11/01/18 09:00 PM (6 years, 2 months ago)

Are those 1/2 gallon, did you PC that big boy?


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Offlinedrunkymonkey
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Re: slowed down to nothing inoculation? [Re: XnMe]
    #25585558 - 11/01/18 09:13 PM (6 years, 2 months ago)

CONTAMINATED?! really? what makes you think?  i should mention also that the black parts are bird seed.. i had some WBS left over so i filled thos things (pickle jars) with horse oats, pressure cooked and then put in the WBS spawn.

can you not innoc two different spawns?

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Invisiblepuff4200
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585563 - 11/01/18 09:16 PM (6 years, 2 months ago)

You'll know if it's contaminated if you shake them and they don't recover, like the other guy said.

Also of those are half gallon jars I hope you extended the pressure cooker time, I personally would have just done 4 qts.

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Offlinedrunkymonkey
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Re: slowed down to nothing inoculation? [Re: puff4200]
    #25585573 - 11/01/18 09:22 PM (6 years, 2 months ago)

if i remember it was in the PC for about 2h 30mins

i shook them up.  guess we will wait and see now

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InvisibleMateja
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585575 - 11/01/18 09:24 PM (6 years, 2 months ago)

Quote:

drunkymonkey said:
CONTAMINATED?! really? what makes you think?  i should mention also that the black parts are bird seed.. i had some WBS left over so i filled thos things (pickle jars) with horse oats, pressure cooked and then put in the WBS spawn.

can you not innoc two different spawns?



1 how long did you PC the oats and at what PSI?

2 have you done g2g successfully before?

3 did you pour the leftovers from the WBS jar you spawned into the oats? Or was the WBS jar used solely for g2g? How was the WBS jar inoculated?

Answering this will help us help you do it right!


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OfflineEp1429
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585585 - 11/01/18 09:27 PM (6 years, 2 months ago)

Length of time in the PC doesn't matter.  Length of time at pressure matters.  You can have the jars in boiling water for ten hours, but if it doesn't pressurize, then it's pointless.

Remember phase diagrams from 7th grade science.  As you increase the pressure in the PC, it allows the water to boil at a hotter temperature.  This increased temp is what kills the spores and bacteria.  You need to start your timer when the PC reaches 15 PSI, and not a moment before.

If simply heating it up to about 200 degrees was all that was required, you could successfully innoc pasteurized food sources.

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InvisibleMateja
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Re: slowed down to nothing inoculation? [Re: Ep1429]
    #25585620 - 11/01/18 09:43 PM (6 years, 2 months ago)

About 15 min of 120C temps is enough to take care of the most stubborn household contaminants.
The reason why we PC big jars for 3+ hours and preferably on 20+ PSI is to make sure that sufficient heat has penetratated into the center of every jar. If your grain is on the dry side and looks light then count on longer PC cycle to get to the center of the jar. If your grain is well hydrated and seems compact then count on shorter duration until center of jar is sufficientky heated. I assume by this point it's clear why we sterilise liquids for 15-20 min only hehe


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Offlinedrunkymonkey
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585659 - 11/01/18 09:59 PM (6 years, 2 months ago)

AH sorry i'm freaking out. ok answers:


1 how long did you PC the oats and at what PSI? 2h30m @ about 18psi

2 have you done g2g successfully before?  yes

3 did you pour the leftovers from the WBS jar you spawned into the oats? Or was the WBS jar used solely for g2g? How was the WBS jar inoculated?

I inoculated the WBS by squirting a spore syringe in a inocc bag i got from amazon with the breathable port thing. when it finished it smelled like fresh mushroom.  i broke it up and distributed it to other jars some quart and those two larger in the picture. i'm not sure on the size exactly but they are large pickle jars.. the largest i've ever seen at grocery stores.  other smaller jars inoculated fine and that is what i put in the 2 tubs i just posted earlier asking about the 2 tubs and the difference in mycelium.

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InvisibleMateja
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Re: slowed down to nothing inoculation? [Re: drunkymonkey]
    #25585710 - 11/01/18 10:31 PM (6 years, 2 months ago)

Ok now I have a good grasp on the procedures you did leading up to this point.

As you might already know, spores are inherently dirty since they are exposed to open air for at least a period of time, at one point. Inoculating grain with bacterial inoculate is a hit and miss and nothing to recommend especially to new growers.


But to g2g spawn that was inoculated with questionable inoculate is practically playing Russian roulette.

AAAANNNNDDDD when you add to that the fact(have no clue how you did this) that you g2g:ed from a spawn bag into jars seems like a risk in itself. Can't imagine myself even trying to g2g from a spawn bag inside a SAB.

At this point it's more than reasonable to assume that your spawn is suffering from all these vectors. But at least you now know how to do it better next time :smile:

Are you interested in working with agar?


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Offlinedrunkymonkey
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Re: slowed down to nothing inoculation? [Re: Mateja]
    #25587125 - 11/02/18 03:26 PM (6 years, 2 months ago)

yeah ive been watching 18 agar dishes.

https://www.shroomery.org/forums/showflat.php/Number/25578688

so far i think they are all doing ok.  i used the syringe one day before the agar and trying to inoc horse oats..  i think only 2 jars out of 10 have any signs of mycelium after about 10 days but the petri dishes all have the pretty white stuff.

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