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OfflineShyftlisiymp
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Registered: 01/31/18
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Negative or positive airflow in clean room
    #25568881 - 10/26/18 10:41 AM (6 years, 2 months ago)

I'm moving soon from a very dirty rental house into basically a new house, fresh paint and cleaned top to bottom. I have the opportunity to build a contained room for all my innoculations including agar. I plan the be able to chemically clean the room and then have an exhaust fan to pull the bleach/iso fumes out a window so I can work. After searching and reading on the forum I know this wont make a room clean enough to treat like I'm working in front of a flow hood, I'll use a sab. My question is if it would be better to have a positive flow with a filter blowing air from the rest of the house and then out a window or a sealed room that i can clean and then quickly exhaust fumes out a window? Does it even matter because the negative pressure will pull air from the rest of the house anyway?

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OfflineChwyn
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Registered: 08/21/16
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Re: Negative or positive airflow in clean room [Re: Shyftlisiymp]
    #25570082 - 10/26/18 07:48 PM (6 years, 2 months ago)

Either way wont be all that effective without a filter. Most commercial labs use positive pressure through a hepa. I used to do SAB work on my kitchen countertop. I wouldnt bother spending money on a large setup that would marginally decrease contamination rate.


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Offlineteladi
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Re: Negative or positive airflow in clean room [Re: Chwyn]
    #25570535 - 10/27/18 12:36 AM (6 years, 2 months ago)

Negative if you don't want what you're working on to get out (TB, Anthrax, etc), positive otherwise.

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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: Negative or positive airflow in clean room (moved) [Re: Shyftlisiymp]
    #25570805 - 10/27/18 07:19 AM (6 years, 2 months ago)

This thread was moved from Advanced Mycology.

Reason:
Sterile technique is a cornerstone of the hobby not an advanced part.

You don't need a fancy room, you need skill. A nice room is a nice room but it won't help you a bit if you don't know how to work in a SAB or with a flow hood. you can use a SAB in a carpeted room with cats roaming around and a litter box. To spend the money on your room you would be so much better off just building a flow hood

If you're using a SAB you want neither positive or negative pressure. You want still air outside of the SAB as well, hence closing vents and no fans etc...

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Offline3rdiopn21
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Registered: 04/29/23
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Loc: Chicagoland
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Re: Negative or positive airflow in clean room (moved) [Re: bodhisatta]
    #28390350 - 07/09/23 01:01 PM (1 year, 6 months ago)

So it's too late, I live in a extremely old and dusty apt. Have been having contamination issues so even though you don't think a tent is necessary bear with me and give me your opinion on which way should I have my air flow.  I purchased a spider farmer 24x24x55 with a full spec. Light and fancy fan. I like toys so I bought it (mid life crisis) perfect fit for my closet and it will piss off my wife lol.

But seriously I need one place for colonizing and fruiting and all automated.(ADHD)
I have trouble keeping steady temps.
I'm thinking positive pressure cause I want filtered air coming in and the pan cyans definitely love FAE.  ??????

Should the light be on full blast or 20,40,60,80 watts?? Your help greatly appreciated.

Edited by 3rdiopn21 (07/09/23 01:05 PM)

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