|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 4 years, 2 months
|
Issues with Contamination when going to grain from Agar
#25561500 - 10/23/18 08:31 PM (6 years, 2 months ago) |
|
|
So I have been growing for years, but always seem to have issues pop up when going to grain from agar. I will have fine agar to agar transfers, but as soon as I transfer to grain my contamination rates skyrocket to 50% or more. I PC my grains for 2 hours in my pressure cooker. I have used both pp5 containers and glass quart jars. Currently I am using glass quart jars with plastic lids and sterile filter discs for gas exchange. I was under the impression that these discs can be reused, but I am worried that maybe the discs are transferring contamination. Is it possible that the discs once exposed to contams will hold and transfer them? Is it possible that upon shaking the jars I am allowing some contams to get through small gaps in the lid or disc due to flexion? I am attaching pics of the lids I am using. I have tested using a lid without a hole and I still got one that contaminated. Right now I am thinking that it is the jar shaking and agitation that is somehow letting contamination get in, but you can see how the discs are stained so I also wonder if that could be it too. Any help? Almost all my jars are contaminating eventually upon shaking or expansion.
|
BLINKfan420
Jedi



Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 6 days
|
Re: Issues with Contamination when going to grain from Agar [Re: BigGreenMat]
#25561594 - 10/23/18 09:00 PM (6 years, 2 months ago) |
|
|
--------------------
 
All my pictures are drawn from imagination. I don't even know what a mushroom is.
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: BigGreenMat]
#25561602 - 10/23/18 09:03 PM (6 years, 2 months ago) |
|
|
Those disks are absolutely reusable even if they get contaminated. Might want to wash em a bit, but, whatever is in there will die in the PC. Im using ones that are all stained from mold right now.
It's pretty darned frustrating to go through what you are going through, all I can suggest is trying metal lids for a run in case there is something going on with those plastic ones.
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: BLINKfan420]
#25561610 - 10/23/18 09:04 PM (6 years, 2 months ago) |
|
|
do you use a SAB? filter disc is good for reuse what filter size are those? 2hrs pc time at what PSI?
ive had terrible run running 2hrs at 15psi for grains, I moved to doing 2-2.5hrs @ 20 psi these days
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
|
Tormato  
Chairman of the Bored




Registered: 07/01/17
Posts: 6,106
Last seen: 3 days, 9 minutes
|
Re: Issues with Contamination when going to grain from Agar [Re: BLINKfan420]
#25561613 - 10/23/18 09:05 PM (6 years, 2 months ago) |
|
|
Are you doing A2G using a flow hood or SAB?
If the disks have been exposed to conatms, as long as you wash the jars and lids and PC correctly they will re-sterilize.
What PSI is your PC set at?
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: pablokabute]
#25561649 - 10/23/18 09:14 PM (6 years, 2 months ago) |
|
|
Quote:
I moved to doing 2-2.5hrs @ 20 psi these days
WTF? Do you live in the Himalayas?
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561655 - 10/23/18 09:15 PM (6 years, 2 months ago) |
|
|
i pc at 18-20psi
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561662 - 10/23/18 09:17 PM (6 years, 2 months ago) |
|
|
Quote:
VroomerMcZoomers said:
Quote:
I moved to doing 2-2.5hrs @ 20 psi these days
WTF? Do you live in the Himalayas?
i live in the subtropics but my guess is my AA pressure gauge is fucked. 2hrs @ 15psi gives me bacterial contam even in the unopened control jars. 2hrs @ 20 psi gives me peace of mind, not to mention perfect sterilization. ive wasted around 50lbs of grains before i made the move.
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
|
Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
|
Re: Issues with Contamination when going to grain from Agar [Re: Tormato]
#25561666 - 10/23/18 09:19 PM (6 years, 2 months ago) |
|
|
If the same sfd and lid keep failing, Toss them. I have had brand new sfd be bad. Once I weened out the bad ones, I was good.
You say sterile filter disc,. Is that a polypropylene or a cellulose/fabric based disc? I can't really tell from picture. Sfd should stand for synthetic filter disc.
|
willwont
A



Registered: 06/05/18
Posts: 11
Last seen: 4 years, 7 months
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561672 - 10/23/18 09:20 PM (6 years, 2 months ago) |
|
|
Just out of curiosity what is the average expected contam rate when transferring from agar to grain vs lc vs li ?
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: pablokabute]
#25561677 - 10/23/18 09:21 PM (6 years, 2 months ago) |
|
|
Ive never seen a PC designed to go above 15 PSI.
Do those All American ones do it or something? Is that what you get for the extra $$$ they cost?
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561696 - 10/23/18 09:26 PM (6 years, 2 months ago) |
|
|
taped some quarters to my rocker so it weighs more. more weight=higher temp=higher psi. you should be able to do that to any pc that uses a weight thingy.
also runs quietly and barely looses any water
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561702 - 10/23/18 09:27 PM (6 years, 2 months ago) |
|
|
Quote:
VroomerMcZoomers said: Ive never seen a PC designed to go above 15 PSI.
Do those All American ones do it or something? Is that what you get for the extra $$$ they cost?
my pc is an old school AA I bought second hand, an antique, the gauge reads 30psi max and vents off pressure once you reach 30psi, vents off until it reads 20psi again, new designs has weights that lets you set the exact pressure before venting off.. if shipping cost werent too prohibitive, id buy a new gauge and maybe 1 wing nut, 1 wing nut of mine got fucked while tightening it up with a pliers xD
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: mushboy]
#25561732 - 10/23/18 09:39 PM (6 years, 2 months ago) |
|
|
What kind of grains are you using? Is it stuff that's made for human consumption?
Or is it horse feed?
Im having trouble understanding why sterilization at 20PSI or 2.5 hours would be necessary.
1.5 hours at 15PSI works great for me. Organic rye I can get cheap from an organic foods distributor.
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25561765 - 10/23/18 09:51 PM (6 years, 2 months ago) |
|
|
Quote:
VroomerMcZoomers said: What kind of grains are you using? Is it stuff that's made for human consumption?
Or is it horse feed?
Im having trouble understanding why sterilization at 20PSI or 2.5 hours would be necessary.
1.5 hours at 15PSI works great for me. Organic rye I can get cheap from an organic foods distributor.
I use whole oats, if organic rye was available here, id be eating that shit so me and the mushies eat the same thing, anything labeled organic here fetches 2-5x the original price, thats if its available(I have seen organic rye thru this elitist shop called Healthy Options)
i live in a 3rd world country where genocide is rampant,
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
Edited by pablokabute (10/23/18 09:53 PM)
|
BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 4 years, 2 months
|
Re: Issues with Contamination when going to grain from Agar [Re: pablokabute]
#25561801 - 10/23/18 10:07 PM (6 years, 2 months ago) |
|
|
I am using a SAB. I have been doing everything I can to ensure no air flow. I wipe down the box and then use soapy water on the walls. I wear gloves and tyvek sleeves and wipe stuff down with 70% alcohol while trying to make sure I never have anything over an open jar or plate. Yes, SFD means synthetic filter discs. My SFDs are from mycosupply.com. I am using whole oats meant for horse consumption. PC is at 15 psi. I am near sea level. Maybe I will try an extra quarter on the steam weight.
-------------------- My Growth So Far!
|
BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 4 years, 2 months
|
Re: Issues with Contamination when going to grain from Agar [Re: BigGreenMat]
#25561840 - 10/23/18 10:16 PM (6 years, 2 months ago) |
|
|
Do you all think that banging the jars when breaking up the grains before doing a G2G or to mix up the mycelium to distribute it evenly in the jar after 30% colonization could allow in contaminants?
-------------------- My Growth So Far!
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: BigGreenMat]
#25561856 - 10/23/18 10:23 PM (6 years, 2 months ago) |
|
|
Quote:
BigGreenMat said: Do you all think that banging the jars when breaking up the grains before doing a G2G or to mix up the mycelium to distribute it evenly in the jar after 30% colonization could allow in contaminants?
when the filter is failing, most probably, ive never used SFDs, just polyester. you can actually cut a small SFD and stick it with a hi temp rtv silicon to ensure a proper seal. Ive never used SFD but been wanting to since Ive seen it.
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
|
Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
|
Re: Issues with Contamination when going to grain from Agar [Re: pablokabute]
#25562383 - 10/24/18 06:06 AM (6 years, 2 months ago) |
|
|
If you can't load a video of your SAB session so we can help figure out what or where it's going wrong, try your best to give us details of your SAB procedures. What are you doing with your lids when you are dropping the ager in to the jar. You setting it down or holding it.
I run my lids the same as you. I shake mine and have no issues with shake.
After you PC, are you letting then cool in the PC or outside? Do you tighten your lids all the way before you PC or leave a little loose?
The more details provided, the better chance we can help you solve this.
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
|
Re: Issues with Contamination when going to grain from Agar [Re: pablokabute]
#25562859 - 10/24/18 09:59 AM (6 years, 2 months ago) |
|
|
Quote:
pablokabute said: 2hrs @ 15psi gives me bacterial contam even in the unopened control jars. 2hrs @ 20 psi gives me peace of mind, not to mention perfect sterilization. ive wasted around 50lbs of grains before i made the move.
It sounds like you're failing to allow steam to escape for several minutes before putting on the weight or closing the toggle. It is very important to get all the air out of the PC and replace it with steam. Otherwise, you're building air pressure rather than steam pressure and the temp will be much lower.
After proper venting, 15 psi for 90 minutes is going to sterilize the grains sufficiently to get them colonized, assuming quart/liter jars. Use two hours for larger spawn bags, and keep them separated so steam can pass between, under and over each bag. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: RogerRabbit]
#25563140 - 10/24/18 11:53 AM (6 years, 2 months ago) |
|
|
What's your opinion about the auto air vents you find on a lot of cookers? Are they effective or should you continue to vent even after they pop up?
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,241
Loc: rural ghetto
|
Re: Issues with Contamination when going to grain from Agar [Re: RogerRabbit]
#25563149 - 10/24/18 11:56 AM (6 years, 2 months ago) |
|
|
Quote:
RogerRabbit said:
Quote:
pablokabute said: 2hrs @ 15psi gives me bacterial contam even in the unopened control jars. 2hrs @ 20 psi gives me peace of mind, not to mention perfect sterilization. ive wasted around 50lbs of grains before i made the move.
It sounds like you're failing to allow steam to escape for several minutes before putting on the weight or closing the toggle. It is very important to get all the air out of the PC and replace it with steam. Otherwise, you're building air pressure rather than steam pressure and the temp will be much lower.
After proper venting, 15 psi for 90 minutes is going to sterilize the grains sufficiently to get them colonized, assuming quart/liter jars. Use two hours for larger spawn bags, and keep them separated so steam can pass between, under and over each bag. RR
I do 10mins on high heat before I close the toggle. Im thinking my gauge is fucked cuz when I made this move, all my problems disappeared, anyway, Im using whole oats bought from a feeds supply store, i pack them in pint jars and my pressure cooker packed to the brim, I can fit 24 jars per batch in my AA921
--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
|
teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 1 year, 5 months
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25563239 - 10/24/18 12:29 PM (6 years, 2 months ago) |
|
|
Quote:
VroomerMcZoomers said: What's your opinion about the auto air vents you find on a lot of cookers? Are they effective or should you continue to vent even after they pop up?

That isn't an auto-vent. It's a pressure indicator and safety feature preventing your from opening the lid while at pressure.
A PC can be at pressure, but still not vented correctly.
--------------------
|
Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
|
Re: Issues with Contamination when going to grain from Agar [Re: teladi]
#25563302 - 10/24/18 12:43 PM (6 years, 2 months ago) |
|
|
Are you getting mold, or bacteria first? If it's bacteria, could simply be prepping too wet
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: teladi]
#25563316 - 10/24/18 12:45 PM (6 years, 2 months ago) |
|
|
--------------------
Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
|
Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25563342 - 10/24/18 12:51 PM (6 years, 2 months ago) |
|
|
Obviously it’s a vent as it vents. It also states it’s a cover lock. Either way, one should still vent steam for 10 min or so, even if the auto vent/cover lock has raised before the 10 min is up.
|
Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25563347 - 10/24/18 12:52 PM (6 years, 2 months ago) |
|
|
I think air can still get caught in though, the valve wouldn't know the difference between air and steam, it's just pressure activated
|
teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 1 year, 5 months
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25563357 - 10/24/18 12:54 PM (6 years, 2 months ago) |
|
|
Read later on in the manual, page 7:
Quote:
7. Exhaust air from the canner and jars by adjusting heat to a relatively high setting to obtain a free flow of steam from the vent pipe. Consult the instruction book which accompanied your range for recommended heat setting. Reduce heat to maintain a moderate steam flow. Allow steam to flow for 10 minutes.
Also, from page 5:
Quote:
When the cover is rotated to the closed position, the air vent/cover lock passes under the locking bracket. When the handles are aligned, one over the other, the pressure regulator is placed on the vent pipe, and heat is applied, pressure will begin to build within the canner.At this point, the air vent/cover lock lifts and locks the unit. The cover will remain locked as long as there is pressure in the canner. When pressure is completely reduced, the air vent/cover lock drops allowing the canner to be opened (Fig. K).
It will not automatically vent all the air in the PC. It does vent, and it will automatically lock, but its purpose isn't just to vent.
--------------------
|
VroomerMcZoomers
"This user is a dumbass"


Registered: 08/11/17
Posts: 1,637
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: Caps McGee]
#25563390 - 10/24/18 01:00 PM (6 years, 2 months ago) |
|
|
Always vent for a few minutes longer after that thing pops up. 10 to be sure. Unless some precise information comes along indicating otherwise.
Edited by VroomerMcZoomers (10/24/18 01:22 PM)
|
Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
|
Re: Issues with Contamination when going to grain from Agar [Re: VroomerMcZoomers]
#25564027 - 10/24/18 04:27 PM (6 years, 2 months ago) |
|
|
15 mins from the time it pops. Never done me wrong.
|
BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 4 years, 2 months
|
Re: Issues with Contamination when going to grain from Agar [Re: Tookitooki]
#25564430 - 10/24/18 06:55 PM (6 years, 2 months ago) |
|
|
Quote:
Tookitooki said: If you can't load a video of your SAB session so we can help figure out what or where it's going wrong, try your best to give us details of your SAB procedures. What are you doing with your lids when you are dropping the ager in to the jar. You setting it down or holding it.
I run my lids the same as you. I shake mine and have no issues with shake.
After you PC, are you letting then cool in the PC or outside? Do you tighten your lids all the way before you PC or leave a little loose?
The more details provided, the better chance we can help you solve this.
In one hand I am lifting the lid and holding the plate. I then use the other hand with the exacto knife to pick up the agar wedge of a good size and drop it into the jar. The jar lid is tipped up a bit, but not set down or moved much.
I am letting them cool in the PC. The lids are on tight with foil on them. I figure that because there is a hole in the lid and the disc under it that this will allow the pressure to equalize and let steam in before I put the weight on.
-------------------- My Growth So Far!
|
BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 4 years, 2 months
|
Re: Issues with Contamination when going to grain from Agar [Re: Caps McGee]
#25564444 - 10/24/18 07:00 PM (6 years, 2 months ago) |
|
|
Quote:
Caps McGee said: Are you getting mold, or bacteria first? If it's bacteria, could simply be prepping too wet 
Generally mold. I will try to take some pics or a video of my next attempt.
-------------------- My Growth So Far!
|
Tormato  
Chairman of the Bored




Registered: 07/01/17
Posts: 6,106
Last seen: 3 days, 9 minutes
|
Re: Issues with Contamination when going to grain from Agar [Re: Tookitooki]
#25564451 - 10/24/18 07:02 PM (6 years, 2 months ago) |
|
|
Quote:
Tookitooki said: 15 mins from the time it pops. Never done me wrong.
Same! That's how I roll. Wait till the steam is only coming out of the main vent then start timing.
-------------------- Helpful Threads
The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
|
MagickMushroom
Wyzard Bard

Registered: 08/24/18
Posts: 93
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: mushboy]
#25584104 - 11/01/18 11:55 AM (6 years, 2 months ago) |
|
|
Mushboy what a great tip. Thanks!
Quote:
mushboy said: taped some quarters to my rocker so it weighs more. more weight=higher temp=higher psi. you should be able to do that to any pc that uses a weight thingy.
also runs quietly and barely looses any water
|
MagickMushroom
Wyzard Bard

Registered: 08/24/18
Posts: 93
Last seen: 6 years, 1 month
|
Re: Issues with Contamination when going to grain from Agar [Re: RogerRabbit]
#25584121 - 11/01/18 12:00 PM (6 years, 2 months ago) |
|
|
RR thhank you for that. Although I haven't had any probs like the ones described in this thread, it is important to know this piece of info. So how many minutes would you recommend the venting period? Maybe 10 to 15 mins before placing the weight on the PC?
also runs quietly and barely looses any water
Quote:
RogerRabbit said:
Quote:
pablokabute said: 2hrs @ 15psi gives me bacterial contam even in the unopened control jars. 2hrs @ 20 psi gives me peace of mind, not to mention perfect sterilization. ive wasted around 50lbs of grains before i made the move.
It sounds like you're failing to allow steam to escape for several minutes before putting on the weight or closing the toggle. It is very important to get all the air out of the PC and replace it with steam. Otherwise, you're building air pressure rather than steam pressure and the temp will be much lower.
After proper venting, 15 psi for 90 minutes is going to sterilize the grains sufficiently to get them colonized, assuming quart/liter jars. Use two hours for larger spawn bags, and keep them separated so steam can pass between, under and over each bag. RR
|
teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 1 year, 5 months
|
Re: Issues with Contamination when going to grain from Agar [Re: MagickMushroom]
#25584202 - 11/01/18 12:28 PM (6 years, 2 months ago) |
|
|
Start timing when there is a steady stream of steam coming out of your vent. 10-15 minutes is typical venting time.
|
Gardennub
Stranger


Registered: 10/24/18
Posts: 58
Loc: Somewhere out there
Last seen: 5 years, 9 months
|
Re: Issues with Contamination when going to grain from Agar [Re: RogerRabbit]
#25602965 - 11/09/18 12:09 PM (6 years, 2 months ago) |
|
|
Quote:
RogerRabbit said:
Quote:
pablokabute said: 2hrs @ 15psi gives me bacterial contam even in the unopened control jars. 2hrs @ 20 psi gives me peace of mind, not to mention perfect sterilization. ive wasted around 50lbs of grains before i made the move.
It sounds like you're failing to allow steam to escape for several minutes before putting on the weight or closing the toggle. It is very important to get all the air out of the PC and replace it with steam. Otherwise, you're building air pressure rather than steam pressure and the temp will be much lower.
After proper venting, 15 psi for 90 minutes is going to sterilize the grains sufficiently to get them colonized, assuming quart/liter jars. Use two hours for larger spawn bags, and keep them separated so steam can pass between, under and over each bag. RR
Whoa this is good to know! Thanks ...i might be cyber stalking you on this board a little right now.
--------------------
|
|