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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Do these cakes look healthy to you? [Re: PrimeNumber]
#25574389 - 10/28/18 04:46 PM (6 years, 2 months ago) |
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-------------------- Cakes inside Water Tub
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PrimeNumber
Student


Registered: 10/18/18
Posts: 184
Last seen: 4 years, 10 months
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Re: Do these cakes look healthy to you? [Re: Mateja]
#25577883 - 10/30/18 02:26 AM (6 years, 2 months ago) |
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Quote:
Mateah said: Here is the PF Tek grow along if anyone wants to join me
https://www.shroomery.org/forums/showflat.php/Number/25574381
Give me a few more days to regroup, I'll be there.
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teladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa
Last seen: 1 year, 5 months
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Re: Do these cakes look healthy to you? [Re: PrimeNumber]
#25577899 - 10/30/18 02:51 AM (6 years, 2 months ago) |
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Quote:
PrimeNumber said: I've thrown out all my cakes. The snowy-white turned green. Definitely trichoderma.
I've done some troubleshooting in my mind as to what went wrong with my previous two batches. Both were clearly contaminated with trichorderma after over a week in the SGFC. There seem to be three likely culprits.
1) Bad syringes, in which case, I should not be reordering the same variety from the same (very well regarded) provider. I think this is unlikely, but certainly possible.
2) Inadequate pressure cooking. If this is indeed the case, that I should be sterilizing for 90-120 minutes instead of 45-60, someone other than myself should have a talk with Ziran and his updated PF Tek advice.
3) Dirty apartment. I have a dog. The trichoderma showed up after over a week in the SGFC; I think it's reasonable that my relatively unventilated, soft-ceiling tile bathroom, landed on the cakes while they were trying to pin.
I can order different spores and pressure cook for longer--and try to grow in my kitchen instead of my bathroom--but if trichoderma shows up after all of that, I'd say my apartment is fucked.
+++
I'm not giving up. But I'm trying to isolate what went wrong, and I have it narrowed down to these three.
Lastly, I'm not doing this hobby to have a good time, I'm doing it because I've been depressed as hell for the last several years, and shrooms actually help me feel better. Failing at the PF Tek is taking a toll on me, but I'm not giving up. I don't feel I have any other choice than to keep trying.
Last thing: Do I toss the perlite? Do I toss the SGFCs?
1. All prints and syringes are dirty. We clean up on agar, or try outgrow contam in PF cakes, or roll the die with direct inoculation.
2. For PF, the 90 minutes is a steam. If you can PC, then you reduce your sterilisation time. How did you PC them?
3. We all have varying levels of contam loads in our environments; I can't keep my cats out of the room I use for SAB work, and it's too damn hot here to keep the windows permanently closed in there. Review your procedures if you think it's your environment, 'cause I'll guarantee it's how you do things.
i.e. I bet it's you.
You will want to clean your SGFC and perlite.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Do these cakes look healthy to you? [Re: teladi]
#25581794 - 10/31/18 01:22 PM (6 years, 2 months ago) |
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Quote:
teladi said:
1. All prints and syringes are dirty.
This is a necessary assumption for high success rates guys...
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