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 VroomerMcZoomers
"This user is a dumbass"
Registered: 08/12/17
Posts: 1,637
Last seen: 2 years, 3 months
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 Peroxide useless? 
#25556344 - 10/21/18 09:34 PM (2 years, 4 months ago) |
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So, I just found what appears to be clean growth coming from pins that I dropped on agar.
These were from coir cakes fruited in very non sterile conditions. In a SAB, I dipped the pins in 3% peroxide for a few seconds before placing on the agar. There was vigorous bubbling.
The growth was slow to come, I thought maybe I killed it, but, there is thick white mycelium growing radially away from the pins now.
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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
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mushboy
Moooshboy?
Registered: 04/24/05
Posts: 24,794
Loc: better trashcan
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unless its an inivto/agar pin, all clone material comes from very non sterile environments. the peroxide is 100% unnecessary.
clean with agar, not peroxide. its why we use it.
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merch137
Genetic Resevoir
Registered: 12/17/17
Posts: 1,072
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: mushboy]
#25556417 - 10/21/18 10:17 PM (2 years, 4 months ago) |
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I'm finding uses for peroxide.
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Those that know their mushrooms say...[...] Amanita.
[quote]sublimistri said:
id probably just trip it out then make them a friend and grow my own belladonna secretly to dose them on. [/quote]
[quote]sublimistri said:
this is the exact reason I wanna grow belladonna, because ima get that bitch back. [/quote]
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mushboy
Moooshboy?
Registered: 04/24/05
Posts: 24,794
Loc: better trashcan
Last seen: 38 minutes, 54 seconds
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Re: Peroxide useless? [Re: merch137]
#25556462 - 10/21/18 10:35 PM (2 years, 4 months ago) |
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Quote:
merch137 said:
I'm finding uses for peroxide.
....they are??
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VroomerMcZoomers
"This user is a dumbass"
Registered: 08/12/17
Posts: 1,637
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: mushboy]
#25556482 - 10/21/18 10:55 PM (2 years, 4 months ago) |
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The inside of a mushroom is very non-sterile?
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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
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 Some Dude
Finding Stuff Out
Registered: 07/14/16
Posts: 6,801
Loc: By myself
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Re: Peroxide useless? [Re: mushboy]
#25556525 - 10/21/18 11:18 PM (2 years, 4 months ago) |
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Quote:
mushboy said:
Quote:
merch137 said:
I'm finding uses for peroxide.
....they are??
I had a conversation with someone yesterday that was re-sterilising plastic petri's with peroxide said it works for him 
Sure yiu could technically use those questionable dishes to make transfers but no way I'm gonna use what came from those dishes as inoculate 
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p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.
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merch137
Genetic Resevoir
Registered: 12/17/17
Posts: 1,072
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: Some Dude]
#25556528 - 10/21/18 11:21 PM (2 years, 4 months ago) |
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It's in my journal I'm reusing petris with it as well as some success with cleaning wild tissue or tissues from contammed dishes with it, it seems the sterile water dip afterwards was more effective than my last attempts of straight peroxide until it goes away off the myc.
As dipping in sterile water would help deactivate the peroxide from sitting there harming the myc the entire time while it starts to rejuvenate on the petri.
--------------------
Those that know their mushrooms say...[...] Amanita.
[quote]sublimistri said:
id probably just trip it out then make them a friend and grow my own belladonna secretly to dose them on. [/quote]
[quote]sublimistri said:
this is the exact reason I wanna grow belladonna, because ima get that bitch back. [/quote]
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mushboy
Moooshboy?
Registered: 04/24/05
Posts: 24,794
Loc: better trashcan
Last seen: 38 minutes, 54 seconds
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Re: Peroxide useless? [Re: merch137]
#25556529 - 10/21/18 11:22 PM (2 years, 4 months ago) |
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Far out
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merch137
Genetic Resevoir
Registered: 12/17/17
Posts: 1,072
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: mushboy]
#25556535 - 10/21/18 11:24 PM (2 years, 4 months ago) |
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Both of these clones are on peroxide recycled dishes
 
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Those that know their mushrooms say...[...] Amanita.
[quote]sublimistri said:
id probably just trip it out then make them a friend and grow my own belladonna secretly to dose them on. [/quote]
[quote]sublimistri said:
this is the exact reason I wanna grow belladonna, because ima get that bitch back. [/quote]
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gizmodo
Woodland Creature
Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 1 month, 9 days
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Quote:
VroomerMcZoomers said:
The inside of a mushroom is very non-sterile?
Larvae and stuff from the outside of the mushroom can easily get into your tissue sample.
Taking a sample from the inside is considered the best idea, but not considered sterile.
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One must be mindful to maintain their balance on the slippery earth.
Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits
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VroomerMcZoomers
"This user is a dumbass"
Registered: 08/12/17
Posts: 1,637
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: gizmodo]
#25556652 - 10/22/18 12:29 AM (2 years, 4 months ago) |
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Granted if a bug gets in there, it's not going to be clean there. But what about when there are no insect problems and it's the inside of a mushroom that you split apart carefully and got a wee sample of flesh from?
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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune. -Noam Chomsky
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merch137
Genetic Resevoir
Registered: 12/17/17
Posts: 1,072
Last seen: 2 years, 3 months
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This was my second 3d printed petri I did a few months back it was done with peroxide and has a transfer in it I opened it up for this pic outside of the sab for a while so it may have gotten contams from that. Was poured 4-5 days ago or so. This is one of the designs done in pla.
 
Material cost is roughly five cents a piece, and about the same for larger plates. I'm probably going to produce better versions and fully translucent versions of these later when I have money to do things like this. You can see the .5-1mm grips they have on top and bottom near my fingers.
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Those that know their mushrooms say...[...] Amanita.
[quote]sublimistri said:
id probably just trip it out then make them a friend and grow my own belladonna secretly to dose them on. [/quote]
[quote]sublimistri said:
this is the exact reason I wanna grow belladonna, because ima get that bitch back. [/quote]
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Some Dude
Finding Stuff Out
Registered: 07/14/16
Posts: 6,801
Loc: By myself
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It's almost irrelevant how clean the inner flesh of a fruit is for two reasons.
First the tissue sample will regenerate fast and outrun most competition (and that's IF the sample is contaminated)
Second you will anyway not use the tissue sample or the plate it is on for anything other than to make another transfer.
So it will anyway get cleaned in one transfer.
(I'm not sure how my Swenglish translates for everyone so let me clarify)
What I mean to say is that you won't use the tissue sample plate for inoculation anyway so it doesn't matter if the tissue same itself is 'sterile' or not (I forgot the fancy word it so I said 'sterile' but if someone is kind to remind me I'll copy paste that word my journal where I remember stuff) I should remember that word cause I've learned it 3-4 times so far...
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p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.
Edited by Some Dude (10/22/18 12:39 AM)
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merch137
Genetic Resevoir
Registered: 12/17/17
Posts: 1,072
Last seen: 2 years, 3 months
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Re: Peroxide useless? [Re: Some Dude]
#25556667 - 10/22/18 12:37 AM (2 years, 4 months ago) |
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Bacteria flooding contammed tissue or even molds, and the tissue does nothing I've seen quite a few times.
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Those that know their mushrooms say...[...] Amanita.
[quote]sublimistri said:
id probably just trip it out then make them a friend and grow my own belladonna secretly to dose them on. [/quote]
[quote]sublimistri said:
this is the exact reason I wanna grow belladonna, because ima get that bitch back. [/quote]
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Some Dude
Finding Stuff Out
Registered: 07/14/16
Posts: 6,801
Loc: By myself
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Re: Peroxide useless? [Re: merch137]
#25556674 - 10/22/18 12:42 AM (2 years, 4 months ago) |
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Yes some tissue will not always regenerate before melting that can be due to bactera, environmental or old/damaged/dying tissue. But when it's regenerating it does that pretty fast.
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p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.
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