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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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3D agar liquid culture/innoculant * 1
    #25531361 -

I use this media bottle because of a recommendation from here. The pour is like magic. Very precise. I like this shape better for pouring because my SAB is small. 250ml is good for like 5 myco-quarts++...

I also like the clarity of the bottle for the needed sight, the measurment, and it being pressurc oookable all in one



You Can PC it, just keep the bottle lid from sealing 100%. You can feel it when tightening. It will be fine sealed too but safety first, it wont melt. Use foil on top so that you can equalize pressure in the bottle after PC.



Outline:

Make grain soak/boil water your base with agar powder. Make it like you're going to pour agar but 1/3rd - 1/2 the amount of agar. I added a small amount of light malt extract as well. Its agar, do what you want.

Let it cool, be patient.

Take a very tiny piece of mycelium off of an agar plate, it can be 1st or 2nd transfer from germination preferably. drop it in and tighten lid. Temperature shouldn't be too warm for this or it may sink and make the next step hard.

Let it grow on top, be very gentle and do not shake. Watch for contams, its still 2D surface.

Give it a day or two to stretch out a little and shake. Shake it hard, don't be a bitch. Give it bubbles.

The bubbles are suspended inside the agar bottle along with a huge amount of microscopic growing mycelium. The surface of the agar is now hundreds of little spheres and growth is RAPID.

You will be able to pour soon. you'll see how much growth you have if you hold it to the light after sitting a long time and settling the bubbles.

If you shake it a day or two after the first shake, your growth is exponential.




Great! So a fast-colonizing innoculant with a sterility test built-in. It could be possible to shoot or drop a tiny amount of spores in and germinate like this too. Just keep your eye out for contams longer(you will anyway because germination is slow).

There is utility to this.

The agar is mostly water, it liquefies but also stays suspended throughout the grain jar. This prevents pooling after using a large amount of the bubble slush. It also re-hydrates your grains, allowing you to keep your grains on the drier side when prepping your grain jars. helps to keep bacteria down and prevent waterlogging.

Using more IS better. You're spreading around buoyant water-filled agar slush(slushier is better than lumpy) saturated with happy already growing mycelium. It itself is useful to your spawn as food and water.

Shake it hard. Don't be a bitch.


This can take you from syringe to grains in one step, feed and hydrate your spawn, and make colonization time a week. I didn't wait more than a week after transfer before I poured the slush to grains. It was more than enough already.


Really fast innoculant/grain colonization, or spore germ to unlimited grain jars(g2g or use less innoculant each).

A T0 liquid culture parent is pretty neat. Might take a couple tries though.

Edited by mushfuss (10/12/18 04:27 AM)

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Re: 3D agar liquid culture/innoculant [Re: mushfuss]
    #25531363 -

:threadmonitor:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: 3D agar liquid culture/innoculant [Re: elasticaltiger]
    #25531404 -

:whathesaid:
I need some pictures


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: 3D agar liquid culture/innoculant [Re: bodhisatta]
    #25531418 -

Well, the proof is in the puddin'

"The proof of the pudding is in the eating!"


Its super late for me, I didn't really prepare much but wanted to share when I realized why its all colonizing so fast.
A couple photos tomorrow.

Edited by mushfuss (10/12/18 05:36 AM)

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Re: 3D agar liquid culture/innoculant [Re: mushfuss]
    #25532577 -

Back with some photos!

Note the lack of controls. I'll be playing with agar nutrients as a control to Guage 2D vs 3D culture growth.


Puddin' Bubble all in one tek
https://imgur.com/a/gzX3xG7

I am really excited about this. Hopefully it plays out consistently.

Grain is at most 3 days after inoculation. Petri dish agar grew slow but I'm not experienced in time-frames.

This wasn't planned, just trying agar dishes for the first time and doing experiments because I have the bottles, PC, and why not. I read how contam prone lc/li is and wanted to see myself.

Shaking was to distribute like in normal liquid cultures where it groups up and swirls, sometimes matts on top where there is air. Bubbles staying suspended in the puddin' were chance, and realizing the bubbles were an important trait of the success popped me out of bed to post at almost 5am.

# of Sterile transfers required are reduced along with potential contam vectors, and speed are vastly improved. Sterile tek is the minimum needed. PC, SAB+70%iso on bare hands.

This reigns Supreme for a noob with spores or a mushroom to clone. Easier than pf cakes and straight to grain.

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