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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Using Wheat to spawn (HELP) [Re: Cody2C]
    #25522637 -

I'm saying that you shouldn't have unused sterilized grain for long periods of time: bacterial endospores not taken care of in the PC will germinate and multiply


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Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25522648 -

I keep mine 5 days before I consider it gone. That's only jars. Bags is like 2 days depending if I've opened the PC.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25523462 -

i read your post, i need to research a lot more, simply because i don't even know how to use agar. should inject spores into it and then use the agar to spawn?


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: Cody2C]
    #25523471 -

ok.. okay, thats.. AWESOME! 4 days for fully colonized jar!! WOW, i didn't know this! alright here's the question, how much of your colonized grain do you transfer to the new jar? and also just to be safe i don't mess it this time, can i colonize BRF cake and then transfer it to the grains? or it have to grains in order to be able to transfer them between jars?


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25523475 -

i'm confused here, what do you mean 'unused sterilized grain for long periods of time'? would you please clarify?


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: van hatton]
    #25523476 -

what are you guys talking about :confused:


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25523835 -

Sterilizing and letting it sit before innoculating... I  don't prep grain until the night before I plan on dropping plates ... most certainly can use brf to inoculate grain, but would still want a clean culture from agar : A2G>G2G seems like it'd be easier


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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25523846 -

Sounds like your grain prep is ok, maybe soak 12 hour instead of 24 but I think that the issue is your inoculation.
Multi-spore to grain is asking for trouble.

Learn agar and isolate a culture, then inoculate your grains.
If you do BRF, simply clone an early pin to agar, make sure its clean and you're working with an isolated fruiting culture.


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:mushroom2::mushroom2::mushroom2:
"When the AC is running full blast & it's still fucking hot - it's time to shut the window"
- nobody

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Re: Using Wheat to spawn (HELP) [Re: Spore-hunter]
    #25523862 -

No need to isolate, just clean it up... I try to keep as much genetic variance in the initial MS tubs as possible... no way of knowing what you're isolating without it fruiting, or what genetics you passed up isolating before fruiting... 2 or 3 quick transfers before going to grain (clean) to have plenty of genetics displayed  in fruit, clone from several favorite  (size, phenotype, whatever) and grow those out... test, and pick from those a culture(s) to slant... furthermore, IME, isolations generally suck, and I've seen better performance from expanding original clone cultures repeatedly, rather than further isolating... to find that one isolate would be cool, but I think it's been said (RR) that only 1 in 12 true isolates are worthy


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Ask Caps! (My Q&A Thread)
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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25595606 -

i took your advice, just brought everything that i need to prepare agar. just wanted to let you know. :thumbup:


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: Spore-hunter]
    #25595617 -

what do you mean of 'isolate a culture?' can you please explain more?


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25595626 -

To isolate a culture it's easier to start with a clone and then you keep transferring until all the growth is 100% even with no sectoring. Here's a good thread on the process of starting a monoculture.

https://www.shroomery.org/forums/showflat.php/Number/24741888/fpart/all/vc/1

If I'm not mistaken this guy started with spores so he had to do more transfers to get a monocture but you should get the point.

Edited by puff4200 (11/06/18 09:00 AM)

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Re: Using Wheat to spawn (HELP) [Re: puff4200] * 1
    #25596603 -

I would concentrate on getting a clean culture... I like doing MS tubs as genetically variant as possible, then pick 5 fruit to clone... side by side in shoeboxes to determine a winning culture based on colonization speed, yeild, and potency


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Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Using Wheat to spawn (HELP) [Re: Caps McGee]
    #25597653 -

alright! correct me if i'm wrong, what you guys are suggesting is after i made my agar plates i should use a drop of my spore syringe or use one of my grain spawn (anyhow) on one of them and let it grow, then take each section separately and fruit them, which ever was better i have to keep isolating..? the term clean culture confuses me, if someone could please describe more! also what is MS tube? sorry i'm new to this :crazy2:


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25597858 -

Like caps said, I'd focus on getting a clean culture. So either of the ways you mentioned would do that but the grain that already has mycellium to agar would be faster.

If your trying to work toward a monoculture then I would definitely start with clones like caps said. If you try to get a monoculture from multispore your going to be doing countless transfers to get a monocture. If you start with clones you'll only do a few to a handful usually. Like caps also said I would just work off a few different mushrooms and make a monocture out of each one rather than try to separate and keep every sector.

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Re: Using Wheat to spawn (HELP) [Re: puff4200]
    #25597974 -

i got that part, the thing is i don't understand 'clones' what do you mean of that?


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25597992 -

When you produce mushrooms you can split it and scrap a bit of the stringy material in the middle and put it on a agar plate. That is a clone.

Edited by puff4200 (11/07/18 07:58 AM)

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Re: Using Wheat to spawn (HELP) [Re: puff4200]
    #25597999 -

aaaaalllriiight now i got it!! thank you! :thumbup:


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: Cody2C]
    #25602333 -

it happened again, this time i took a picture, this is the contamination i talked about, as you can see after a 3-4 weeks it didn't colonize the top parts, why is that?


last time it happened i removed the contaminated parts and spawned the rest into bulk substrate, i din't expect anything to grow, after 1.5months (i leaved that bag alone) i saw lots of pins growing, i moved it to a box and they grow, almost half way, one of them grow big. is it safe to eat that?!


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Know thyself, and thou shalt know the universe and God

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Re: Using Wheat to spawn (HELP) [Re: pezhvak]
    #25602370 -

Looks bacterial, just because something is bacterial dosent mean it won't grow though. The mycellium won't take hold of the seeds on the top because the bacterial already has. Notice how your mycellium is thick and creamy so much so you can't see the seeds through it? That's a very good sign of bacteria. Like I said before though you can spawn it and you may still get something.

Any mushroom you get is safe to eat for the most part (unless its slimy/ rotten)

I'd either work on your sterile procedure more or take a good look at your cultures because you could have hidden bacteria on them. If you used spores instead of a culture then that's obviously the problem.

https://www.shroomery.org/forums/showflat.php/Number/22020260/fpart/all/vc/1

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