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OfflineGrey6Bard
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Popping My Shroom
    #25478697 - 09/21/18 06:05 PM (6 years, 3 months ago)

So this will be my first attempt at ever growing mushrooms. I did research years ago but never went through with it as I wasn’t in a stable living situation. On a whim I decided that I was tired of waiting to try growing some powerful fungi. I ordered several strains from the spore depot. Should have them by October. This thread will be my attempt at documenting my first attempt at growing this glorious fruit. I believe I have the read and watched enough to attempt a very novice grow set up at the least. I would like to be as professional as possible but I need the experience so I’m diving in. If anyone has any tips I would greatly appreciate that. I may be blowing my load by creating this thread but the excitement of this task undergoing in the near future cannot be staved.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25478746 - 09/21/18 06:16 PM (6 years, 3 months ago)

Good luck:thumbup: My advice would be to spend a week reading the forums and teks before you go any further so you are sure of what you want to do and how, and ask some questions before doing it as its your first grow

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Re: Popping My Shroom [Re: Grey6Bard]
    #25478755 - 09/21/18 06:18 PM (6 years, 3 months ago)

Everything you need to grow is here https://www.shroomery.org/forums/showflat.php/Number/24144021

If you haven’t got a pressure cooker, you can follow the pf tek and make a sgfc - just follow these vids here


If you’ve got a pressure and you’re feeling confident after reading everything, get some agar, jars and grains and grow a monotub or shoebox.

Anyway the tip is to read everything in that link above.

Good luck Gray Bard

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Re: Popping My Shroom [Re: space_shrooms]
    #25478875 - 09/21/18 06:50 PM (6 years, 3 months ago)

I went with MS inoculation to rye to bulk sub with my first (current) grow and ran into a few issues and learned a hell of lot.

Underestimate how clean you are. Clean spawn is the goddamn key. Whether you're going for PF Tek or grain spawn to bulk substrate, read up very carefully on how to prep, plan out your steps and know that you can never be too clean. Sterile technique and clean spawn will take care of a lot of potential issues.

If you're going for grain spawn, I recommend checking out Bodhisatta's Easy AF Oat Prep Tek, except, if you use rye, I would only boil for about 8-10m. Longer than that and I started seeing way too many bursted kernels.

Read up on proper surface conditions. Keep an eye on that and only do enough to maintain good surface conditions and you will be in a good place.

Don't fuck with your grow. You'll be tempted to check everything a million times if you're as curious and impatient as me. Make sure your sub is hydrated (field capacity if you're spawning to bulk), case if you'd like, and leave it the hell alone until you absolutely need to do something.

Light is good for mycelial growth (12h on/12h off like fruiting conditions). Liners aren't necessary.

If you need help, don't be afraid to ask. I asked every possible question that came up that I couldn't find an answer to through the search function. Hell, if you have questions for my noob ass, feel free to PM. I know nothing. But I grew mushrooms muahaha.

Oh yeah, GOOD LUCK!! :cheers:


--------------------
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Edited by thegiftedchild (09/21/18 06:52 PM)

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: thegiftedchild]
    #25478965 - 09/21/18 07:21 PM (6 years, 3 months ago)

Thanks for the advice guys, I’ll be reading the links that you’ve provided! I’ve watched several videos but I definitely need some hands on experience. I’m going to follow this https://www.google.com/amp/s/thethirdwave.co/psychedelics/shrooms/grow-psilocybin-mushrooms/amp/ how do you guys feel about this? It seems very simple and a quick way to learn and acquire some experience and knowledge of the life cycle of the mushrooms. I definitely feel like I need some hands on experience on a very simple level so I can understand the life of the mushroom. I’m super excited to get started. On my first try I’m just going to focus on  being sterile, learning the life cycle, and trying to gain the most knowledge I can before attempting other techniques. How many tries does it usually take for a noob like I to get a decent yield?

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Grey6Bard]
    #25479548 - 09/21/18 11:56 PM (6 years, 3 months ago)

After reading some of those posted links above I have a couple questions...

I’ve seen grow set ups where they’ve either put bags over the mushrooms or lids upturned to allow some air flow but it left me curious, does sterility become less of factor after colonization? My other question is, for doing a shoebox set up, am I correct in assuming that you chop or grind up the cake and apply it to a substrate and let it cultivate within the shoebox?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25479554 - 09/22/18 12:07 AM (6 years, 3 months ago)

Quote:

Grey6Bard said:
After reading some of those posted links above I have a couple questions...

I’ve seen grow set ups where they’ve either put bags over the mushrooms or lids upturned to allow some air flow but it left me curious, does sterility become less of factor after colonization? My other question is, for doing a shoebox set up, am I correct in assuming that you chop or grind up the cake and apply it to a substrate and let it cultivate within the shoebox?




Yes, sterility is not a consideration after colonization. Once the sterilized grains are colonized, the mycelium has essentially taken over the sterile space, leaving no room for contaminants to set in. This of course depends on whether the grains were properly sterilized and no other culture other than myc was introduced to them. You can spawn colonized grains to pasteurized bulk substrate in open air, without gloves etc., with no problems. Clean spawn added to properly pasteurized substrate should not contaminate for a few flushes at least.

Yes, you can crumble up a cake into a bulk substrate. Or you can just fruit it as it is, which is what I'd recommend for your first time. When you crumble a cake into CVG (coir, verm, gypsum), you are expanding the culture and the amount of nutrients and water it can feed off. This will result in more shrooms :pipesmoke:

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Re: Popping My Shroom [Re: space_shrooms] * 1
    #25479665 - 09/22/18 02:09 AM (6 years, 3 months ago)

If you are going to follow the thirdwave tek do not wipe the syringe after flaming or wipe between jars. Just flame it.


--------------------


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Bumholio]
    #25481091 - 09/22/18 03:17 PM (6 years, 3 months ago)

Thanks, guys! This looks so easy! I’m so excited. I don’t know why I didn’t cultivate sooner! I may even start a gourmet op!!!

@Bumholio, should I flame it before every jar?

Also, is it better to use distilled water or just boiled tap water? Does the mycelium get the nutrients it needs from plain distilled water? I know there is an excess of junk like chlorine and fluoride in tap that it doesn’t need but does boiling remove these chems?

Edited by Grey6Bard (09/22/18 03:46 PM)

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Offlinespace_shrooms

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Re: Popping My Shroom [Re: Grey6Bard]
    #25481231 - 09/22/18 04:33 PM (6 years, 3 months ago)

You can use regular tap water. Don’t need to boil it because you’ll be sterilizing it anyway.

There’s a bunch of bs in that third wave tek about air sanitizers and making your work space as ‘sterile’ as possible. You can’t make your workspace even close to sterile. I’d highly recommend making one of these and working inside it - https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888

Flame the syringe beteeen each jar. Don’t spray it with anything once it’s red hot. The first thing it should be making contact with after coming out of the flame is the substrate inside the jar.

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Re: Popping My Shroom [Re: space_shrooms]
    #25481250 - 09/22/18 04:40 PM (6 years, 3 months ago)

That’s exactly what I was going to do but the comments helped me. I was gonna try drilling but I guess I’ll just melt the holes. I haven’t heard back from spore depot but it’s a Saturday so we will see what’s up on Monday, I hope!

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Re: Popping My Shroom [Re: Grey6Bard]
    #25481559 - 09/22/18 07:20 PM (6 years, 3 months ago)

For the first trial I am going to do hands on inoculation. I’m going to try two or three different methods including in jar, shoebox and the original tek I posted which seems a bit outdated but a good one to try nonetheless. On my second trial I’m looking into agar, how should I go into to it as a beginner? I’ve read some things but what’s a basic starting point and where can I get the supplies?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25484106 - 09/23/18 09:40 PM (6 years, 3 months ago)

I have another basic question, lol, sorry.

In between flushes do I need to do anything?

Do I need to rehydrate the cake by dunking?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25484129 - 09/23/18 09:55 PM (6 years, 3 months ago)

Yes 12-18 hours


--------------------


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Re: Popping My Shroom [Re: Grey6Bard]
    #25484242 - 09/23/18 11:49 PM (6 years, 3 months ago)

Quote:

Grey6Bard said:
For the first trial I am going to do hands on inoculation. I’m going to try two or three different methods including in jar, shoebox and the original tek I posted which seems a bit outdated but a good one to try nonetheless. On my second trial I’m looking into agar, how should I go into to it as a beginner? I’ve read some things but what’s a basic starting point and where can I get the supplies?



You're anxious and excited which is good. Agar is quite easy with Pastyplates. I recommend reserving a few drops from your syringes and getting started right away. You'll have nothing to do for a while but check on your jars 10 times a day. Starting agar will buffer that a little and bump you up to the next level.


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Grimsweeper]
    #25487760 - 09/25/18 10:51 AM (6 years, 3 months ago)

Spores should be here by Friday according to my email! I ordered extra syringes for experimenting with agar and for storage so no problem there!

I like that agar tek particularly the food coloring idea! I was actually gonna go ahead and start this as well in the mean time. I may use that technique but I like the idea of ordering Petri dishes as they would take up less room and look a bit more professional. Although I don’t know I may try that as it doesn’t involve pouring agar in a SAB, we will see!

A couple quick noob questions on the Bods shoebox tek...
It mentions after the first flush misting or dunking. If you have grated the cake into new substrate and mixed it then you allow it to colonize but wouldn’t that create the risk of contamination when you add the 80% colonized sub to the new sub? Or do you sterilize it first? Also if you mix the two, I’m assuming you wait until 100% colonization before you flip the lid? How do you dunk the cake after the first flush? Remove it from the shoebox? Doesnt that stress the mycelium?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25487863 - 09/25/18 11:39 AM (6 years, 3 months ago)

I don't know where you're reading about adding a 80% colonized sub into a new sub I think you're mistaken. Crumble fully colonized brf cakes into a substrate like CVG. The Shoe Box would be a good choice because you probably don't have enough cakes to have enough spawn to do anything larger. Ratio should be one part spawn to one part substrate or at the least one part spawn to two parts substrate.

For dunking after a first flush you can pour water down the side of the shoebox, leave it sit for 4 hours or so then drain it out. Your sub is fairly sturdy by this point you can tilt it on its side if you're careful.

I don't flip the lid until I see knots or pins. Some people do it earlier but a shoe box is pretty maintennamce free if you let it be. If your conditions are good you won't even have to mist it until later flushes.

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Re: Popping My Shroom [Re: Grimsweeper]
    #25488872 - 09/25/18 06:51 PM (6 years, 3 months ago)

Maybe I did misread.

I have everything on order. Will be shipped by October 3rd, may recieve a few things on the fourth. I’m slightly paranoid about the fact that I ordered most of everything that I needed online. I couldn’t find hardly any of it at Walmart and that’s about all we have in our little town. I prefer to shop online but that did sketch me out slightly. Whatever, I kinda wanna order some gourmet mushroom spores anyways but I wanna see if I’m handicapapable of doing this first. I spent maybe 250$ estimate on everything including the spores. Some of the stuff we already have at home.

I think I have a good grasp on things now in my head at least. I really need some hands on experience as soon as I can, I have to keep reading information or I’ll forget what I learned just like I did 8 years ago. I never did try 8 years ago but I did do a lot of reading. I was younger and dumber then though. Lol. It’s definitely going to be hard seeing those spores in the fridge and not being able to inoculate any of those jars until the rest of my equipment comes, haha.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25489091 - 09/25/18 08:13 PM (6 years, 3 months ago)

Good luck bud it's a fun hobby, psycodelics or not.


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Grimsweeper]
    #25489252 - 09/25/18 09:43 PM (6 years, 3 months ago)

Y’all are so helpful! Thank y’all seriously!

Another dumb question on agar...

Say I take a culture, to preserve it do I store it in the fridge immediately or do I keep it in room temperature to allow the myc to colonize? Does storing it in the fridge reduce its metabolic rate or is it completely suspended? Sorry for the dumb question.

I realize liquid nitrogen would probably freeze it for an indefinite amount of time but I’m assuming a fridge slows it’s growth to a crawl and it would still go bad or die off over time?

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OfflineDreamReality
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Re: Popping My Shroom [Re: Grey6Bard]
    #25489367 - 09/25/18 10:42 PM (6 years, 3 months ago)

Agar > LI/LC > Grain > Monotub

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Re: Popping My Shroom [Re: DreamReality]
    #25489599 - 09/26/18 02:48 AM (6 years, 3 months ago)

I tend to store generations of plates in the fridge whilst I'm waiting to ensure the next gen plates have taken and are clean transfers, slows their growth down a lot..Find leaving them in there for two long tends to dry them out, plus fridges aren't that clean. Look into slants for long term storage, also had good results with grain masters over a period of 12-18 months, few grains taken back to agar to clean up:thumbup:

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Shroomspective]
    #25490165 - 09/26/18 10:07 AM (6 years, 3 months ago)

Ha! So much to learn. I’ll look into slants after I get my first projects going. I should have my spores by tomorrow as they arrived in the nearest city this morning. So I imagine they will be dropped off today or tomorrow. I use a pack and ship, I hope that causes no issues.

I still won’t be able to inoculate until the rest of my equipment comes, ugh, what a bummer.
I’m so stoked!!!

I feel I’ve garnered a lot of knowledge over the past week or two!

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Re: Popping My Shroom [Re: Grey6Bard]
    #25503491 - 10/01/18 01:48 PM (6 years, 3 months ago)

I got a little impatient, the mail is just taking too long. So I went out and picked up some vermiculite, I couldn’t find BRF so I got some WBS. Do I need to change the proportions for this third wave tek with WBS? I’m currently grinding some up and prepping my jars.

If all goes well I will inoculate them tomorrow then I may start another 12 jars with the BRF just to garner some experience with alternate variables.

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OfflineDreamReality
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Re: Popping My Shroom [Re: Grey6Bard]
    #25503633 - 10/01/18 02:39 PM (6 years, 3 months ago)

You don't grind WBS. use the FooMan Tek to prep it


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: DreamReality]
    #25503861 - 10/01/18 04:17 PM (6 years, 3 months ago)

I’ve read that some folks do grind the WBS as a substitute when they couldn’t find any BRF. I’m gonna go through with it since I have six jars already prepped and The jars I bought are a strange shape, I’m not sure if I’ll be able to get a cake out. Maybe I’ll just do all of these jars this way and use them for shoeboxes then try BRF cakes tomorrow after I get off work, my order has arrived just have to go pick it up. Anyways, If this trial doesn’t work I’m only losing out on one syringe.

I bought 8 just to experiment with different methods so no big loss.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25505931 - 10/02/18 11:32 AM (6 years, 3 months ago)

So update on my status, I’ve inoculated 6 jars. I had planned on the girlfriend helping me but our busy schedules didn’t allow it so I felt very rushed and in my anxiousness I think I did a shit job. If I get anything from these first six jars I would be thoroughly impressed.

I’m going to try to take my time on the next batch but after this one I’m kinda let down. I had to rush through all the steps. I was anxious and actually jabbed myself with one of the syringes HA, spores in my DNA. I had to switch needles. I didn’t realize how easy you had to press the syringe and I dumped a lot of CCs into the first two jars on accident.

This first batch was a total learning experience and I botched it completely. No harm though but it was a bit frustrating especially having to rush things because of time restraints.

Total luck if I even get any mycelium to colonize.
Disappointed in myself.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25506022 - 10/02/18 12:07 PM (6 years, 3 months ago)

So you're telling me theres a chance!

I did some pretty stupid stuff when i first tried, hindsight is always 20/20 and you'll have so many things you know you can improve on next time, you should be one with the shroom now that you've been inoculated.

I've found this hobby takes patience above all else.


If some/one of those does take you may be able to speed up the next round if you're feeling bold enough for a g2g.

...I wonder if mushrooms will start to grow out of your.. *use imagination*

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Re: Popping My Shroom [Re: whosthisguy]
    #25507388 - 10/02/18 08:08 PM (6 years, 3 months ago)

Yeah, I’m considering starting another six jars tonight and then on my next day off doing my first tek that I planned on doing all along. The one that I feel is the most simple and slowly so I can be more thorough. I already got these six rolling so I figure maybe I’ll just try an get em inoculated.


Maybe I’ll just try wait till my day off after all seeing as we don’t have water today, haha...
Frustrating place that I live.

Oh and yeah, I’ll have the biggest penis envy.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25510595 - 10/03/18 10:00 PM (6 years, 3 months ago)

I inoculated the other six now I’m sterilizing 9 BRF cakes!

Things are going mush smoother this time around! Haha.
I except that out of 21 jars I’ll at least get one going! Lol
I’ve learned a lot. Next day off I’m going to experiment with agar so here’s to nothin!

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Re: Popping My Shroom [Re: Grey6Bard]
    #25517128 - 10/06/18 12:30 PM (6 years, 3 months ago)

21 jars. I see something in my WBS jars, I see some strange red contamination in all six of my crushed WBS jars. Nothing yet in my BRF jars which I have the most hope for but it’s only been two days so I didn’t expect anything yet. Wish I knew how to upload pics. Will figure it out sometime soon if I get the time. I also will prob work on my agar on my next day off...Tuesday.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25517521 - 10/06/18 03:17 PM (6 years, 3 months ago)

Quote:

I also will prob work on my agar on my next day off...Tuesday.




Think ahead to your future grain choice at this early stage and either simmer/boil or soak the grain as the agar nutrients. Strain it helps to see clearly, I recommend that. Food coloring is fun but I probably won't be using it a second time unless I start an organizational system with it.

Using the grain water from germination will encourage the strains that like your specific conditions to grow fastest. If you make a monoculture of something that already likes it's food you're presumptuously more efficient than if you didn't.

I read the teks for this, but I feel highlighting the purpose is just as important as a recipe.

You have plenty of syringes to do this with from germination, so starting sooner is always nice. Make that patience as easy as it can be.

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Re: Popping My Shroom [Re: mushfuss]
    #25523218 - 10/08/18 10:16 PM (6 years, 3 months ago)

So what tek should I begin with for agar?

I’ve got potato flakes and the powdered agar?...
Will that suffice?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25523221 - 10/08/18 10:17 PM (6 years, 3 months ago)



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Re: Popping My Shroom [Re: DreamReality]
    #25528125 - 10/10/18 08:37 PM (6 years, 3 months ago)

I’ve got Petri dishes. Do they work in the same way? I know the things he uses have holes in them. Or should I try a different tek specifically for the Petri dishes?

Also, out of 21 jars so far I find that I’ve had a higher success rate than failure rate. I’m seeing growth on over half the jars and contaminations in only 6 thus far.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25528364 - 10/10/18 10:16 PM (6 years, 3 months ago)

Sorry for this premature bump of my thread but I’d like to share some pics and get some input.



First is my BRF cake, it’s been maybe 8 or 9 days since inoculation. The second is the ground WBS experiment. The third is just wild bird seed. I need some input on how they all look for the amount of time passed any tips would be greatly appreciated.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25528426 - 10/10/18 11:03 PM (6 years, 3 months ago)

Hi Grey6,

Here is a good place to start.

https://www.shroomery.org/forums/showflat.php/Number/24297804

I recommend you look at the bottom of the op at Timethyl’s link on what it should and shouldn’t look like.

I’m not the greatest at analyzing pictures, bad screen or eyes, but they don’t look good from my angle.  What did you make your BRF jars with?  Why is it so white?  Thinking that it couldn’t be colonized like that in 8 days.


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Re: Popping My Shroom [Re: SunnyDayze]
    #25529612 - 10/11/18 11:42 AM (6 years, 3 months ago)

The first pic is just BRF and vermiculite. I don’t know why the BRF looks so white but is not colonization. The other WBS jars were inoculated a couple days before the BRF and are just starting to show some mild progress in colonization. Thanks for the link, will check it out in my spare time.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25529687 - 10/11/18 12:14 PM (6 years, 3 months ago)

1st jar is mold. The other 2 look fucked too but I don't know what color you started off with.


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Re: Popping My Shroom [Re: Grimsweeper]
    #25529713 - 10/11/18 12:29 PM (6 years, 3 months ago)

I don’t think the first jar is mold but I’m a total beginner. I can’t identify anything in it but a small bit of perhaps colonization. The second does look fucked. The third looks okay. I can take more pics of other jars but I’m waiting to see some more before I show em off.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25530831 - 10/11/18 08:25 PM (6 years, 3 months ago)

In the third picture just below the mycelium it looks very dark grey, bacterial, unless that’s just a shadow....
I’m a noob too but trying to help and could be wrong.  Grimsweeper knows more and is probably right though.


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Re: Popping My Shroom [Re: SunnyDayze]
    #25532307 - 10/12/18 01:25 PM (6 years, 3 months ago)

You guys were right, I had to throw away all of my jars except the 9 BRF cakes. Those all seem to be doing well, there seems to be a contamination in one but not well formed enough to make a judgement call. I have hope for the BRF cakes but I really need to get into agar. I need to get a day off to try it.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25532586 - 10/12/18 03:18 PM (6 years, 3 months ago)

If I could go back. I'd never do brf, I would have started with agar and never would have tried pf tek.


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Re: Popping My Shroom [Re: DreamReality]
    #25533119 - 10/12/18 07:52 PM (6 years, 3 months ago)

Agar is so much fun.


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Re: Popping My Shroom [Re: DJmonkeyballer]
    #25533345 - 10/12/18 09:53 PM (6 years, 3 months ago)

Yeah, I will most likely agree after I try it but I wanted to try the basics first. I really do need to get into agar ASAP though. Seems very fun!

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Re: Popping My Shroom [Re: Grey6Bard]
    #25533499 - 10/12/18 11:36 PM (6 years, 3 months ago)

Ordering sterile syringes and growing off of PF cakes was my first go. I viewed agar as some advanced technique, but looking back now I wish I'd started with it.


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Re: Popping My Shroom [Re: DJmonkeyballer]
    #25533590 - 10/13/18 12:49 AM (6 years, 3 months ago)

So an unfortunate update...

More than half of my jars have been invaded by maggots. Yes, maggots.
I have four jars left that look fairly healthy. I think I kinda just screwed the pooch when I applied the micropore tape or when I did the holes for the syringe. Either way maggots infested my jars...

So I’m pretty bent out of shape. I put a lot into this and turns out it wasn’t enough.
I’m discouraged and probably won’t try again for some time.

It’s very unfortunate because I kind of felt like I needed this in my life at this specific time but I suppose not, I’m going to get a second job and forget all about the magic in the mushrooms for another year or two. Next time I will invest in a pressure cooker and try agar from the start. Peace guys...

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Re: Popping My Shroom [Re: Grey6Bard]
    #25533705 - 10/13/18 03:57 AM (6 years, 3 months ago)

We popped your shroom now you're just going to turn your back and walk away after just a few dates? I feel like I just got date raped. Got to give it another go man, it's the right thing to do. Don't be date-rapey Grey6Bard.


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Re: Popping My Shroom [Re: Grey6Bard]
    #25533735 - 10/13/18 04:41 AM (6 years, 3 months ago)

Quote:

Grey6Bard said:
So an unfortunate update...

More than half of my jars have been invaded by maggots. Yes, maggots.
I have four jars left that look fairly healthy. I think I kinda just screwed the pooch when I applied the micropore tape or when I did the holes for the syringe. Either way maggots infested my jars...

So I’m pretty bent out of shape. I put a lot into this and turns out it wasn’t enough.
I’m discouraged and probably won’t try again for some time.

It’s very unfortunate because I kind of felt like I needed this in my life at this specific time but I suppose not, I’m going to get a second job and forget all about the magic in the mushrooms for another year or two. Next time I will invest in a pressure cooker and try agar from the start. Peace guys...




Okay dude please describe your process a bit more. If your jars have maggots then you aren't properly sterilizing them because flys don't burrow through dry vermiculite to lay eggs. Nothing to do with your micropore tape unless you had jars with grains directly exposed to the air with no filter or barier.

I just got into this thread, can you sum up what you currently have available for good jars and bad jars? My understanding is you have 9 brf jars that are still good?

Can we see those? The more pictures you can post of all your stuff, not just the questionable jars, then the more well be able to help you salvage stuff.

I'm sorry you had to throw away jars. It's hard at first but I promise it gets easier. Also don't ever let fear of contamination keep you from trying something.

You definitely have mold in  the jars with the red shit.

Dont worry about getting a picture for every single jar, just post the whole lot in one picture with any colonized areas facing forward.

Well getcha through this.


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Re: Popping My Shroom [Re: elasticaltiger]
    #25533957 - 10/13/18 08:13 AM (6 years, 3 months ago)

Don't put all your eggs in one basket. My first grow attempt was 10 brf jars. 9 contaminated super hard. I put way too much focus on that one batch. Then I started doing one or two new batches every week because I realized I wasn't going to get better anytime soon by doing a batch a month.


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Re: Popping My Shroom [Re: elasticaltiger]
    #25534412 - 10/13/18 12:13 PM (6 years, 3 months ago)

I think they were gnat larvae. We have a problem with gnats in western NC and it doesn’t help that we live around livestock. It’s not terrible but at any given time I can see one or two flying around the room. The place where I tried to seal the tape didn’t seal properly on a lot of the jars, I think that’s how they got into my jars. As of right now I have 1 BRF jar that may be good. I think it’s contaminated possibly, it’s tinged a little yellow in areas. So far I see no maggots though. I also had the layer of vermiculite. All of the jars had mycelium growing but produced maggots after nearly two weeks of colonizing. I will post my last jar...hang on.

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Re: Popping My Shroom [Re: DreamReality]
    #25534421 - 10/13/18 12:16 PM (6 years, 3 months ago)

Quote:

Grimsweeper said:
We popped your shroom now you're just going to turn your back and walk away after just a few dates? I feel like I just got date raped. Got to give it another go man, it's the right thing to do. Don't be date-rapey Grey6Bard.




Lol, yeah I didn’t know it would hurt that bad.
Quote:

DreamReality said:
Don't put all your eggs in one basket. My first grow attempt was 10 brf jars. 9 contaminated super hard. I put way too much focus on that one batch. Then I started doing one or two new batches every week because I realized I wasn't going to get better anytime soon by doing a batch a month.




It’s hard for me to do it like that. I work a lot and there are people I live with that I’d prefer not to know what’s going on so I have to time it right. Not that they would care but they’d be mad that I didn’t tell em and if I did tell em I’d have to worry about them trying to take some, lol.

Let me take a pic of the last jar that I have left.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25534436 - 10/13/18 12:26 PM (6 years, 3 months ago)

I used flash to bring out the yellow color a bit...http://

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Re: Popping My Shroom [Re: Grey6Bard]
    #25536271 - 10/14/18 09:19 AM (6 years, 3 months ago)

Bump for pic views

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Re: Popping My Shroom [Re: Grey6Bard]
    #25536811 - 10/14/18 01:13 PM (6 years, 3 months ago)

Quote:

Grey6Bard said:
I used flash to bring out the yellow color a bit...http://




That seperation line across the center is kinda troublesome. I'm thinking more bacteria.

How did you sterilize your jars?


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Re: Popping My Shroom [Re: elasticaltiger]
    #25536820 - 10/14/18 01:16 PM (6 years, 3 months ago)

Bacterial AF

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Re: Popping My Shroom [Re: Caps McGee]
    #25537141 - 10/14/18 03:15 PM (6 years, 3 months ago)

I sterilized with steam in a pot for an hour and a half. I guess it just isn’t enough. I’ll have to get a PC. I don’t think I can cultivate without one.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25537254 - 10/14/18 03:57 PM (6 years, 3 months ago)

May be prepping too wet initially? Excess moisture generally causes bacterial infection IME

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Re: Popping My Shroom [Re: Caps McGee]
    #25537760 - 10/14/18 07:30 PM (6 years, 3 months ago)

Some of them were definitely too wet, it was obvious after inoculation.
However some weren’t that wet just gotta get some practice down I suppose.

I really do need to try agar and get a pressure cooker.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25538984 - 10/15/18 09:59 AM (6 years, 3 months ago)

I siccessfully ran cakes with just a pot. I would steam for 2 hours every time and I used masking tape to seal the holes while steaming. I would inject right through the tape.


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Re: Popping My Shroom [Re: elasticaltiger]
    #25551289 - 10/19/18 09:13 PM (6 years, 2 months ago)

I wasn’t going to try again for quite a while but...I’m considering trying agar this weekend as some folks are out of the house for the weekend. I’ve got the ingredients specified for pasty whites agar tek but I’m not sure, I also got Petri dishes and the like to go the extra mile. Can I make agar with the pasty whites tek ingredients and still use a pour agar tek? I’m really frustrated with it. I want to this to work out but after all the failures I’m kinda doubting myself.

I wasn’t able to procure a pressure cooker either...

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Re: Popping My Shroom [Re: Grey6Bard]
    #25552221 - 10/20/18 10:08 AM (6 years, 2 months ago)

I use standard MEA agar, with anywhere between 5-9g of agar powder to 500ml of water, depending on west I'm using it for... I can't attend no pour plates

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Re: Popping My Shroom [Re: Caps McGee]
    #25552900 - 10/20/18 02:57 PM (6 years, 2 months ago)

Can I still use the potato flakes as a nutriention source?

Edited by Grey6Bard (10/20/18 02:58 PM)

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Re: Popping My Shroom [Re: Grey6Bard]
    #25552917 - 10/20/18 03:03 PM (6 years, 2 months ago)

Yes... any agar recipe will work for any application... I use soft agar for spore germination and A2G plates, and regular (10g agar powder to 500ml of water) for A2A transfers... though I used soft agar last transfer set and may start doing that: in the interest of resource conservation

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Re: Popping My Shroom [Re: Caps McGee]
    #25553179 - 10/20/18 05:09 PM (6 years, 2 months ago)

Okay awesome I’m wondering how that equates into the ratios by using a bottle to pour into.

I don’t know I will figure it out when I get off work, lol.
I just hope I can get the agar sterile by steam...

That’s really putting me off, how can I go about it to ensure sterilization?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25555211 - 10/21/18 11:04 AM (6 years, 2 months ago)

No pressure cooker?

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Re: Popping My Shroom [Re: Caps McGee]
    #25555395 - 10/21/18 12:34 PM (6 years, 2 months ago)

No...

I’m still trying to borrow one. I may go to Walmart after work today but I can’t afford to spend loads of money at the moment. I just left my good job and I bought a newish car lol.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25556190 - 10/21/18 06:31 PM (6 years, 2 months ago)

Thrift shops or Craigslist

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Re: Popping My Shroom [Re: Caps McGee]
    #25556558 - 10/21/18 09:35 PM (6 years, 2 months ago)

Well I got the only pressure cooker I could find at Walmart, a 80$ 23 quart. Hopefully it will suffice my small scale cultivation op for at least a year...if I even manage to make it work unlike last time.

I’m probably going to give it a go tonight. I can inoculate as soon as the agar is in the Petri and at room temp or does it take longer to solidify? God I wish I had some chem experience.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25556601 - 10/21/18 09:56 PM (6 years, 2 months ago)

It solidifies and cools down in 5-10 min depending on how thick you pour.


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Re: Popping My Shroom [Re: Mateja]
    #25558376 - 10/22/18 05:03 PM (6 years, 2 months ago)

Well I gave it a shot, left it in the PC overnight as it was very late. Had to reheat it to liquefy it. The last bit was thick and goopy. I’m unsure how it’ll turn out. I guess we will see. I’m not expecting much. I don’t think I’m in the right back environment to cultivate. I’m in no position to be meticulous, thorough, patient, and as sterile as I’d like. We will see.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25558442 - 10/22/18 05:22 PM (6 years, 2 months ago)

Too much doubt mane... you got this! :thumbup:

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Re: Popping My Shroom [Re: Grey6Bard]
    #25558497 - 10/22/18 05:41 PM (6 years, 2 months ago)

That's one of the better ones if you're on a budget for sure. You are going to be able to load up 7 to 10 jars in that bad boy and rock. Super easy use and maintenance. Good luck!

Quote:

Grey6Bard said:
Well I got the only pressure cooker I could find at Walmart, a 80$ 23 quart. Hopefully it will suffice my small scale cultivation op for at least a year...if I even manage to make it work unlike last time.

I’m probably going to give it a go tonight. I can inoculate as soon as the agar is in the Petri and at room temp or does it take longer to solidify? God I wish I had some chem experience.



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Re: Popping My Shroom [Re: MagickMushroom]
    #25559037 - 10/22/18 08:29 PM (6 years, 2 months ago)

That’s good to know, I’ve learned a lot, guys!

Will update on the agar in a week or two.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25570443 - 10/26/18 11:08 PM (6 years, 2 months ago)

So I have some growth also have some small contamination. Agar seems to have made this much easier, I will try to make some more plates in the next couple days.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25571102 - 10/27/18 10:04 AM (6 years, 2 months ago)



Look good?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25572577 - 10/27/18 08:58 PM (6 years, 2 months ago)



Bump for input...sorry.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25572806 - 10/27/18 10:54 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:


Bump for input...sorry.




The colony at 11 o’clock looks good. If that’s an area that you put spores then it’s a good bet. Make a transfer and see how it does.

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Re: Popping My Shroom [Re: Pastywhyte]
    #25573516 - 10/28/18 09:10 AM (6 years, 2 months ago)

Thanks man! It is, you can see where I’ve used the syringe if you look closely.

I’m gonna have to make so more agar plates to transfer to.
I’m glad I took the chance to do agar and not give up when I tried to inoculate with spore syringe. I have a lot more hope with this method of cultivation. I’ve had a lot of fun doing it too.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25573520 - 10/28/18 09:12 AM (6 years, 2 months ago)

Don’t wrap your plates with tape next time, gonna be a pain to get off. Use cling wrap or parafilm.

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Re: Popping My Shroom [Re: Pastywhyte]
    #25573554 - 10/28/18 09:21 AM (6 years, 2 months ago)

Yeah, I’ll have to order some parafilm. Tapes all I had.

I found out what the cause of my contamination was in my room, kinda embarrassing cause I don’t do shit like this often but it was a half can of wet dog food I left out. So pissed about that...

I’m considering ordering an outdoor burner to sterilize things outside of the house cause I’m having a hard time sneaking a noisey pressure cooker on and off the stove for hours in the middle of the night and it’s raised questions. I think they know what’s going on and they’re not stupid but as long as I’m quiet about it I don’t think they care. I just don’t like prying eyes, lol.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25573655 - 10/28/18 10:14 AM (6 years, 2 months ago)

Glad wrap cut into inch thick rolls is better/less of a PITA imo

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Re: Popping My Shroom [Re: Caps McGee]
    #25573789 - 10/28/18 11:34 AM (6 years, 2 months ago)



Here’s another one, an obvious contamination in the corner.
I need to make more plates, I’ve got the next two days off so there’s a shot!

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Re: Popping My Shroom [Re: Grey6Bard]
    #25573899 - 10/28/18 12:31 PM (6 years, 2 months ago)

Sorry for the DP but I did some reading I guess I’ll wait until tomorrow and transfer all my “good” colonies into 2-3 healthy agar dishes then I will run some more agar and do a B+ strain. Getting excited again, I enjoy working with agar.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25573914 - 10/28/18 12:40 PM (6 years, 2 months ago)

Good luck :smile: always fun to get a nice culture going :super:


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Re: Popping My Shroom [Re: iux]
    #25577289 - 10/29/18 08:09 PM (6 years, 2 months ago)

I’m gonna run some more tomorrow for sure.  What’s up with this one though?



The two colonies look oddly different.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25581819 - 10/31/18 01:31 PM (6 years, 2 months ago)

One on the left is mold... make first transfers from germ plates as soon as you can confirm growth of the species you desire... get away from that shit now! Lol

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Re: Popping My Shroom [Re: Caps McGee]
    #25582189 - 10/31/18 04:07 PM (6 years, 2 months ago)

I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.

Wish me luck! Will report back in a week or so...

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Re: Popping My Shroom [Re: Grey6Bard]
    #25583304 - 11/01/18 03:30 AM (6 years, 2 months ago)

Quote:

Grey6Bard said:
I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.

Wish me luck! Will report back in a week or so...




The only way to remove a contam is to transfer clean mycelium to a new plate.


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Re: Popping My Shroom [Re: DreamReality]
    #25589341 - 11/03/18 03:14 PM (6 years, 2 months ago)

Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25589344 - 11/03/18 03:16 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.




To my understanding you can do an A2G transfer without the dry verm layer as long as you have a filter on your lid.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25589361 - 11/03/18 03:27 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.





Dude, what tek are you doing that says to use dry verm for grains?


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Re: Popping My Shroom [Re: Bumholio]
    #25589372 - 11/03/18 03:33 PM (6 years, 2 months ago)

I’m using BRF as grain, not dry verm. Lol, I think you read that wrong. I’m using micro pore tape as a filter.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25589394 - 11/03/18 03:42 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
im trying to do a agar to grain transfer.




Lol, I think you wrote it wrong:shrug:


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Re: Popping My Shroom [Re: Grey6Bard]
    #25589745 - 11/03/18 06:08 PM (6 years, 2 months ago)

Agar to BRF is what I meant. My bad.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25590902 - 11/04/18 06:58 AM (6 years, 2 months ago)

Don't put verm in grain... Filter the lid

Quote:

Grey6Bard said:
Agar to BRF is what I meant. My bad.



You can drop a wedge in a small jar of sterilized water to make LI, and inoculate with a clean syringe... with or without verm, always a good idea to filter the lid... not sure how you'd A2G BRF with a dry verm layer anyhow :shrug:

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Re: Popping My Shroom [Re: Caps McGee]
    #25591106 - 11/04/18 08:56 AM (6 years, 2 months ago)

Well I learned something today, thanks bud.

My experiment is screwed. Maybe I’ll try again next month.
Once a month experiment and fuck ups thread.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25592022 - 11/04/18 04:30 PM (6 years, 2 months ago)

Ah,  not necessarily... but we learn more from failure than successful happenstance

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Re: Popping My Shroom [Re: Grey6Bard]
    #25593103 - 11/05/18 04:28 AM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Well I learned something today, thanks bud.

My experiment is screwed. Maybe I’ll try again next month.
Once a month experiment and fuck ups thread.




Stop throwing that intention out there.  Do your studying, make a plan and do it!  I had failures in the beginnning and it was all due to not doing my homework.  I still have a bad jar or a plate go bad etc and that is just part of it.  Caps is right, we do learn more from our failures.  Shake off the negative bullshit and have some fun with it. 
:tongue2:


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Re: Popping My Shroom [Re: SunnyDayze]
    #25593475 - 11/05/18 09:29 AM (6 years, 2 months ago)

Quote:

azur said:
Everyone gets contamination sometimes



Quote:

bodhisatta said:
:whathesaid:
Even spawn labs with hoods and fancy boot washing stations




Cracks me up every time but it's 100% true. Just study, talk with others and before you'll know it you'll have a less than 5-10% contam rate


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Re: Popping My Shroom [Re: DreamReality]
    #25605062 - 11/10/18 09:57 AM (6 years, 2 months ago)

My jars tipped over so my BRF cakes got soaked.
I do t have a lot of hope for them but they seem to be doing okay, will take pics sometime today and share them. However, my agar shows absolutely no contamination’s on any of the plates so I’m super stoked about them. The only plate that contained a contam was the GT agar that I transferred. I’m really trying to get a clean GT plate or I risk losing the strain.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25607342 - 11/11/18 02:38 PM (6 years, 2 months ago)

I just got 8 more jars going 4 agar plates and 2 liquid cultures so I’m pretty happy but this has taken me literally like 5 and a half hours, I’m kinda exhausted and although I’m enjoying the process. I really need to come back down to reality as fun as it is, it’s a lot of work, lol.

It wouldn’t be so bad if I could do em in the kitchen like most of you guys or have a lab but no, I drew the short end of the stick, lol.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25607437 - 11/11/18 03:36 PM (6 years, 2 months ago)

Will take a while. But with the time you will get more and more equipment and so the growing procedures will becoming easier.

I got my pressure cooker after my third growth btw, so just chill and let the shroom do its thing :mushroom2:

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Re: Popping My Shroom [Re: Mycelsky]
    #25607443 - 11/11/18 03:40 PM (6 years, 2 months ago)

Quote:

Mycelsky said: just chill and let the shroom do its thing :mushroom2:



Best advice ever...

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Re: Popping My Shroom [Re: Caps McGee]
    #25609954 - 11/12/18 04:24 PM (6 years, 2 months ago)











These are pics of the jars that tipped.

What’s everyone’s opinion, can I save em?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25610101 - 11/12/18 05:15 PM (6 years, 2 months ago)

:horrified:
These are not healthy... any of them... I wouldn't invest anymore time into them personally... very obvious bacterial infection, and mold in most

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Re: Popping My Shroom [Re: Grey6Bard]
    #25610276 - 11/12/18 06:05 PM (6 years, 2 months ago)

Ugh...I’m not very good at this...

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Re: Popping My Shroom [Re: Grey6Bard]
    #25610285 - 11/12/18 06:08 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Ugh...I’m not very good at this...




I did PF tek about 10 years ago, aren't you supposed to have a dry verm layer to the top? Or is this something other than PF?


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Re: Popping My Shroom [Re: CzM8]
    #25610356 - 11/12/18 06:42 PM (6 years, 2 months ago)

Quote:

CzM8 said:
Quote:

Grey6Bard said:
Ugh...I’m not very good at this...




I did PF tek about 10 years ago, aren't you supposed to have a dry verm layer to the top? Or is this something other than PF?




No, I’ve tried that with no success. I tried to colonize these jars with agar plates.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25610375 - 11/12/18 06:48 PM (6 years, 2 months ago)

If Nitrogoxygen  can do it, you can to. Believe brother.


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Re: Popping My Shroom [Re: Grey6Bard]
    #25610381 - 11/12/18 06:50 PM (6 years, 2 months ago)

It's a common feeling when starting this hobby.

I've thought both "I got this on lock" and "I've got no fucking idea" almost simultaneously.

Reality has always been somewhere in between the two.

Realize that even those with top technique expect some level of contamination and failure and only get there after repetition and attention to detail.

1) I'm pretty sure that's why i enjoy it so much
2) The learning curve is steep, but so is the drop off
3) Hindsight, patience, and searching the f*** out of this forum constantly reveals flaws in what i'm doing.

I'd say the number one lesson I've learned is to heed the advice of those that have lived the process more than myself. Almost every grow I've had go sideways tracks back to a shortcut that is successful in some cases but yields disaster when a variable changes.

The solution has almost always been avoid the shortcut and risk or accept you can't control exactly where the dice land given current situation and move forward from there.

--An amateur mycology philospher

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Re: Popping My Shroom [Re: CzM8]
    #25610579 - 11/12/18 08:15 PM (6 years, 2 months ago)

Quote:

CzM8 said:
If Nitrogoxygen  can do it, you can to. Believe brother.




:lol:


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Re: Popping My Shroom [Re: DreamReality]
    #25611120 - 11/13/18 03:15 AM (6 years, 2 months ago)

I want to see another round of pictures of those jars. Some definitely look bacterial but some also look really wet.

What are you using to filter the air in the jars?


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Edited by elasticaltiger (11/13/18 03:17 AM)

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Re: Popping My Shroom [Re: elasticaltiger]
    #25611223 - 11/13/18 06:03 AM (6 years, 2 months ago)

I threw away one that was obviously bacterial.

The others are just too wet, they tipped over in the PC.
It was just a stupid mistake, I should have done more jars to make the PC more stable.

Will share a few more pics tonight.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25612047 - 11/13/18 12:50 PM (6 years, 2 months ago)







Here are three quick pics I took.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25612351 - 11/13/18 03:35 PM (6 years, 2 months ago)

1st ones definitely fucked, the other 2 aren't far behind I'd say.


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Re: Popping My Shroom [Re: Bumholio]
    #25612389 - 11/13/18 03:56 PM (6 years, 2 months ago)

Wouldn't count on any of them personally, start something new, even if you're going to give these a shot

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Re: Popping My Shroom [Re: Caps McGee]
    #25612666 - 11/13/18 06:13 PM (6 years, 2 months ago)

I’ve already got 8 more going already.

Lol, man I really suck at this.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25612685 - 11/13/18 06:22 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
I’ve already got 8 more going already.

Lol, man I really suck at this.





I just threw out 10 bags of ruined sawdust substrate and 2 bags of grains that were past their prime by a month.

The first bulk grow of MS I did (that worked) used a syringe of spores and grain jars that I sterilized. I just shot ~1CC in each jar in a SAB. Are you using a SAB?

Also, as far as having 8 more going... I'm pretty sure thats what this hobby require. It requires your time, and your patients at the same time.


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Re: Popping My Shroom [Re: CzM8]
    #25612740 - 11/13/18 06:39 PM (6 years, 2 months ago)

Yeah, I’m using a SAB.

My problem I think is the house I live in, it’s definitely not contamination free.

So, the light fluffy colonies is mold and the stark white is mycelium?

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Re: Popping My Shroom [Re: Grey6Bard]
    #25612780 - 11/13/18 06:54 PM (6 years, 2 months ago)

The house doesn't matter, no house is even close to contam free. Its the technique that needs to be looked at. Maybe you should record yourself doing your work :shrug:


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Re: Popping My Shroom [Re: CzM8]
    #25613320 - 11/14/18 12:17 AM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Yeah, I’m using a SAB.

My problem I think is the house I live in, it’s definitely not contamination free.

So, the light fluffy colonies is mold and the stark white is mycelium?




Quote:

CzM8 said:
The house doesn't matter, no house is even close to contam free. Its the technique that needs to be looked at. Maybe you should record yourself doing your work :shrug:




Dude, I'd actually be embarrassed to post a picture of some of the rooms of my house. Sterile tek is what's important,  not the house.


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Re: Popping My Shroom [Re: Bumholio]
    #25614462 - 11/14/18 01:41 PM (6 years, 2 months ago)

Yeah, the house I’m living in is fairly shit.
Hopefully I can get a loan and move out next eat.

But I thought my sterilization tek was pretty good.
I get impatient but damn...

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OfflineCzM8
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Re: Popping My Shroom [Re: Grey6Bard]
    #25614696 - 11/14/18 03:12 PM (6 years, 2 months ago)

Something is off if it keeps happening. In RR’s videos he talks about living in a mold infested apartment.


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: CzM8]
    #25614877 - 11/14/18 04:37 PM (6 years, 2 months ago)

Well I’ve made stupid mistakes but maybe I’m accidentally cultivating mold by not being able to see the difference...I don’t know.

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Re: Popping My Shroom [Re: Grey6Bard]
    #25617106 - 11/15/18 06:29 PM (6 years, 2 months ago)

So I don’t know what’s wrong with my sterile technique but my agar plates have a decent success rate. I made four the other day and only one shows sign of contamination. Last time I made agar, most of them progressed without troubles. I dont know...

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Re: Popping My Shroom [Re: Grey6Bard]
    #25617125 - 11/15/18 06:35 PM (6 years, 2 months ago)

25% isn't good. I have over a hundred petris. only a couple show poor growth(other than a couple nasty swabs that I have).

4 Plates isn't going to take you far im(limited)e.


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: CzM8]
    #25617141 - 11/15/18 06:39 PM (6 years, 2 months ago)

Yeah, I realize that, lol.

I’m going to get my own house next year so I can do myc work daily.

EDIT: I did eight jars, two LCs and the only reason I only did four “Petris” is because I used a different container for my agar as I wanted to experiment with agar containers.

Edited by Grey6Bard (11/15/18 06:41 PM)

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Grey6Bard]
    #25619110 - 11/16/18 02:56 PM (6 years, 2 months ago)

This one got a bit wet...



[image]http://www.shroomery.org/forums/thumbs/18-46/240362077-thumb_4DD50E13-1247-4852-A733-DA1ADEE0CD17.jpg[/image]

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Grey6Bard]
    #25621053 - 11/17/18 01:49 PM (6 years, 2 months ago)

How are my mold jars coming along?

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InvisibleBumholio
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Re: Popping My Shroom [Re: Grey6Bard]
    #25621163 - 11/17/18 02:58 PM (6 years, 1 month ago)

Quote:

Grey6Bard said:
How are my mold jars coming along?



Lol,


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"great things may come to those who wait, but only what's left by those who hustle"

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