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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Yeah, I’ll have to order some parafilm. Tapes all I had.
I found out what the cause of my contamination was in my room, kinda embarrassing cause I don’t do shit like this often but it was a half can of wet dog food I left out. So pissed about that...
I’m considering ordering an outdoor burner to sterilize things outside of the house cause I’m having a hard time sneaking a noisey pressure cooker on and off the stove for hours in the middle of the night and it’s raised questions. I think they know what’s going on and they’re not stupid but as long as I’m quiet about it I don’t think they care. I just don’t like prying eyes, lol.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Popping My Shroom [Re: Grey6Bard]
#25573655 - 10/28/18 10:14 AM (6 years, 2 months ago) |
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Glad wrap cut into inch thick rolls is better/less of a PITA imo
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Here’s another one, an obvious contamination in the corner. I need to make more plates, I’ve got the next two days off so there’s a shot!
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Re: Popping My Shroom [Re: Grey6Bard]
#25573899 - 10/28/18 12:31 PM (6 years, 2 months ago) |
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Sorry for the DP but I did some reading I guess I’ll wait until tomorrow and transfer all my “good” colonies into 2-3 healthy agar dishes then I will run some more agar and do a B+ strain. Getting excited again, I enjoy working with agar.
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iux
Tubaeformis boi


Registered: 03/20/18
Posts: 288
Loc: Norway
Last seen: 2 months, 29 days
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Re: Popping My Shroom [Re: Grey6Bard]
#25573914 - 10/28/18 12:40 PM (6 years, 2 months ago) |
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Good luck always fun to get a nice culture going
-------------------- Remember to be respectful to each other, be kind, and enjoy life in a responsible way
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Re: Popping My Shroom [Re: iux]
#25577289 - 10/29/18 08:09 PM (6 years, 2 months ago) |
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I’m gonna run some more tomorrow for sure. What’s up with this one though?

The two colonies look oddly different.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Popping My Shroom [Re: Grey6Bard]
#25581819 - 10/31/18 01:31 PM (6 years, 2 months ago) |
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One on the left is mold... make first transfers from germ plates as soon as you can confirm growth of the species you desire... get away from that shit now! Lol
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.
Wish me luck! Will report back in a week or so...
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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
Last seen: 4 months, 7 days
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Re: Popping My Shroom [Re: Grey6Bard]
#25583304 - 11/01/18 03:30 AM (6 years, 2 months ago) |
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Quote:
Grey6Bard said: I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.
Wish me luck! Will report back in a week or so...
The only way to remove a contam is to transfer clean mycelium to a new plate.
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.
If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly. I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 11 months, 19 days
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Re: Popping My Shroom [Re: Grey6Bard]
#25589344 - 11/03/18 03:16 PM (6 years, 2 months ago) |
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Quote:
Grey6Bard said: Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.
If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly. I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.
To my understanding you can do an A2G transfer without the dry verm layer as long as you have a filter on your lid.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Popping My Shroom [Re: Grey6Bard]
#25589361 - 11/03/18 03:27 PM (6 years, 2 months ago) |
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Quote:
Grey6Bard said: Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.
If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly. I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.
Dude, what tek are you doing that says to use dry verm for grains?
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"great things may come to those who wait, but only what's left by those who hustle"
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Re: Popping My Shroom [Re: Bumholio]
#25589372 - 11/03/18 03:33 PM (6 years, 2 months ago) |
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I’m using BRF as grain, not dry verm. Lol, I think you read that wrong. I’m using micro pore tape as a filter.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Popping My Shroom [Re: Grey6Bard]
#25589394 - 11/03/18 03:42 PM (6 years, 2 months ago) |
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Quote:
Grey6Bard said: im trying to do a agar to grain transfer.
Lol, I think you wrote it wrong
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"great things may come to those who wait, but only what's left by those who hustle"
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Re: Popping My Shroom [Re: Grey6Bard]
#25589745 - 11/03/18 06:08 PM (6 years, 2 months ago) |
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Agar to BRF is what I meant. My bad.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Popping My Shroom [Re: Grey6Bard]
#25590902 - 11/04/18 06:58 AM (6 years, 2 months ago) |
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Don't put verm in grain... Filter the lid
Quote:
Grey6Bard said: Agar to BRF is what I meant. My bad.
You can drop a wedge in a small jar of sterilized water to make LI, and inoculate with a clean syringe... with or without verm, always a good idea to filter the lid... not sure how you'd A2G BRF with a dry verm layer anyhow
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Grey6Bard
Stranger

Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
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Well I learned something today, thanks bud.
My experiment is screwed. Maybe I’ll try again next month. Once a month experiment and fuck ups thread.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Popping My Shroom [Re: Grey6Bard]
#25592022 - 11/04/18 04:30 PM (6 years, 2 months ago) |
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Ah, not necessarily... but we learn more from failure than successful happenstance
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SunnyDayze
Finder of shrubberies



Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: Popping My Shroom [Re: Grey6Bard]
#25593103 - 11/05/18 04:28 AM (6 years, 2 months ago) |
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Quote:
Grey6Bard said: Well I learned something today, thanks bud.
My experiment is screwed. Maybe I’ll try again next month. Once a month experiment and fuck ups thread.
Stop throwing that intention out there. Do your studying, make a plan and do it! I had failures in the beginnning and it was all due to not doing my homework. I still have a bad jar or a plate go bad etc and that is just part of it. Caps is right, we do learn more from our failures. Shake off the negative bullshit and have some fun with it.
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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
Last seen: 4 months, 7 days
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Quote:
azur said: Everyone gets contamination sometimes
Quote:
bodhisatta said:
 Even spawn labs with hoods and fancy boot washing stations
Cracks me up every time but it's 100% true. Just study, talk with others and before you'll know it you'll have a less than 5-10% contam rate
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