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OfflineGrey6Bard
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Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
Re: Popping My Shroom [Re: Pastywhyte]
    #25573554 - 10/28/18 09:21 AM (6 years, 2 months ago)

Yeah, I’ll have to order some parafilm. Tapes all I had.

I found out what the cause of my contamination was in my room, kinda embarrassing cause I don’t do shit like this often but it was a half can of wet dog food I left out. So pissed about that...

I’m considering ordering an outdoor burner to sterilize things outside of the house cause I’m having a hard time sneaking a noisey pressure cooker on and off the stove for hours in the middle of the night and it’s raised questions. I think they know what’s going on and they’re not stupid but as long as I’m quiet about it I don’t think they care. I just don’t like prying eyes, lol.

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Popping My Shroom [Re: Grey6Bard]
    #25573655 - 10/28/18 10:14 AM (6 years, 2 months ago)

Glad wrap cut into inch thick rolls is better/less of a PITA imo

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OfflineGrey6Bard
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Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
Re: Popping My Shroom [Re: Caps McGee]
    #25573789 - 10/28/18 11:34 AM (6 years, 2 months ago)



Here’s another one, an obvious contamination in the corner.
I need to make more plates, I’ve got the next two days off so there’s a shot!

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OfflineGrey6Bard
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Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
Re: Popping My Shroom [Re: Grey6Bard]
    #25573899 - 10/28/18 12:31 PM (6 years, 2 months ago)

Sorry for the DP but I did some reading I guess I’ll wait until tomorrow and transfer all my “good” colonies into 2-3 healthy agar dishes then I will run some more agar and do a B+ strain. Getting excited again, I enjoy working with agar.

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Offlineiux
Tubaeformis boi


Registered: 03/20/18
Posts: 288
Loc: Norway
Last seen: 2 months, 29 days
Re: Popping My Shroom [Re: Grey6Bard]
    #25573914 - 10/28/18 12:40 PM (6 years, 2 months ago)

Good luck :smile: always fun to get a nice culture going :super:


--------------------
Remember to be respectful to each other, be kind, and enjoy life in a responsible way :happyheart:


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OfflineGrey6Bard
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Posts: 222
Loc: North Carolina
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Re: Popping My Shroom [Re: iux]
    #25577289 - 10/29/18 08:09 PM (6 years, 2 months ago)

I’m gonna run some more tomorrow for sure.  What’s up with this one though?



The two colonies look oddly different.

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Popping My Shroom [Re: Grey6Bard]
    #25581819 - 10/31/18 01:31 PM (6 years, 2 months ago)

One on the left is mold... make first transfers from germ plates as soon as you can confirm growth of the species you desire... get away from that shit now! Lol

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OfflineGrey6Bard
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Registered: 09/21/18
Posts: 222
Loc: North Carolina
Last seen: 5 years, 8 months
Re: Popping My Shroom [Re: Caps McGee]
    #25582189 - 10/31/18 04:07 PM (6 years, 2 months ago)

I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.

Wish me luck! Will report back in a week or so...

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OfflineDreamReality
MycoPilot
I'm a teapot


Registered: 03/31/09
Posts: 477
Last seen: 4 months, 7 days
Re: Popping My Shroom [Re: Grey6Bard]
    #25583304 - 11/01/18 03:30 AM (6 years, 2 months ago)

Quote:

Grey6Bard said:
I made a transfer and tried to remove contamination’s from the others that had a little contamination. I haven’t had the time to do another batch of agar but I plan to tomorrow. I was going to today but it’s Halloween. I’m going to sterilize some jars as well and try to start some jars again.

Wish me luck! Will report back in a week or so...




The only way to remove a contam is to transfer clean mycelium to a new plate.


--------------------
Dream's Mono Flip Tek


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OfflineGrey6Bard
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Re: Popping My Shroom [Re: DreamReality]
    #25589341 - 11/03/18 03:14 PM (6 years, 2 months ago)

Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.

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OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 11 months, 19 days
Re: Popping My Shroom [Re: Grey6Bard]
    #25589344 - 11/03/18 03:16 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.




To my understanding you can do an A2G transfer without the dry verm layer as long as you have a filter on your lid.

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InvisibleBumholio
What's the craic
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Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: Popping My Shroom [Re: Grey6Bard]
    #25589361 - 11/03/18 03:27 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Alright well in today’s list of fuck ups and experiments, im trying to do a agar to grain transfer. I didn’t use a layer of dry verm as a contam barrier because I was unsure of how to transfer with the barrier. I’m unsure of how this will turn out but I’m sure you can tell me I’m an idiot, lol.

If this doesn’t work I’m taking a break, it’s hard to find a whole day for mycelium, sadly.
I’ll try again once I move though. Too many prying eyes, makes me a bit paranoid.





Dude, what tek are you doing that says to use dry verm for grains?


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Bumholio]
    #25589372 - 11/03/18 03:33 PM (6 years, 2 months ago)

I’m using BRF as grain, not dry verm. Lol, I think you read that wrong. I’m using micro pore tape as a filter.

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InvisibleBumholio
What's the craic
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Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: Popping My Shroom [Re: Grey6Bard]
    #25589394 - 11/03/18 03:42 PM (6 years, 2 months ago)

Quote:

Grey6Bard said:
im trying to do a agar to grain transfer.




Lol, I think you wrote it wrong:shrug:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

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OfflineGrey6Bard
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Last seen: 5 years, 8 months
Re: Popping My Shroom [Re: Grey6Bard]
    #25589745 - 11/03/18 06:08 PM (6 years, 2 months ago)

Agar to BRF is what I meant. My bad.

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Popping My Shroom [Re: Grey6Bard]
    #25590902 - 11/04/18 06:58 AM (6 years, 2 months ago)

Don't put verm in grain... Filter the lid

Quote:

Grey6Bard said:
Agar to BRF is what I meant. My bad.



You can drop a wedge in a small jar of sterilized water to make LI, and inoculate with a clean syringe... with or without verm, always a good idea to filter the lid... not sure how you'd A2G BRF with a dry verm layer anyhow :shrug:

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OfflineGrey6Bard
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Re: Popping My Shroom [Re: Caps McGee]
    #25591106 - 11/04/18 08:56 AM (6 years, 2 months ago)

Well I learned something today, thanks bud.

My experiment is screwed. Maybe I’ll try again next month.
Once a month experiment and fuck ups thread.

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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
Posts: 14,357
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Re: Popping My Shroom [Re: Grey6Bard]
    #25592022 - 11/04/18 04:30 PM (6 years, 2 months ago)

Ah,  not necessarily... but we learn more from failure than successful happenstance

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InvisibleSunnyDayze
Finder of shrubberies
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Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Popping My Shroom [Re: Grey6Bard]
    #25593103 - 11/05/18 04:28 AM (6 years, 2 months ago)

Quote:

Grey6Bard said:
Well I learned something today, thanks bud.

My experiment is screwed. Maybe I’ll try again next month.
Once a month experiment and fuck ups thread.




Stop throwing that intention out there.  Do your studying, make a plan and do it!  I had failures in the beginnning and it was all due to not doing my homework.  I still have a bad jar or a plate go bad etc and that is just part of it.  Caps is right, we do learn more from our failures.  Shake off the negative bullshit and have some fun with it. 
:tongue2:


--------------------

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OfflineDreamReality
MycoPilot
I'm a teapot


Registered: 03/31/09
Posts: 477
Last seen: 4 months, 7 days
Re: Popping My Shroom [Re: SunnyDayze]
    #25593475 - 11/05/18 09:29 AM (6 years, 2 months ago)

Quote:

azur said:
Everyone gets contamination sometimes



Quote:

bodhisatta said:
:whathesaid:
Even spawn labs with hoods and fancy boot washing stations




Cracks me up every time but it's 100% true. Just study, talk with others and before you'll know it you'll have a less than 5-10% contam rate


--------------------
Dream's Mono Flip Tek


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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains   PhytoExtractum Buy Bali Kratom Powder   North Spore Cultivation Supplies   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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