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 RomeoPapa
Jackass of All Trades
 
Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 5 months
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 Re: Pastywhyte's Easy Agar Tek  [Re: Hydropimp]
#25474201 - 09/20/18 12:40 AM (5 years, 6 months ago) |
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I use the nexus/3M tape as well. 2 layers.
Trust the TEK.
Do it just like its written down.
This agar bit a total game changer!
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It's better to have it and not need it
Than it is to need it and not have it.
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Hydropimp
Stranger
Registered: 08/04/18
Posts: 44
Last seen: 5 years, 2 months
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Re: Pastywhyte's Easy Agar Tek [Re: RomeoPapa]
#25474383 - 09/20/18 04:46 AM (5 years, 6 months ago) |
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Thanks PAPA
And i did the tek as is. Waiting a couple of days for the condensation to clear
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,654
Loc: Exact Center
Last seen: 1 day, 11 hours
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Re: Pastywhyte's Easy Agar Tek [Re: RomeoPapa]
#25474774 - 09/20/18 08:46 AM (5 years, 6 months ago) |
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Quote:
RomeoPapa said:
I use the nexus/3M tape as well. 2 layers.
Trust the TEK.
Do it just like its written down.
This agar bit a total game changer!
Transpore is no good, micropore is What you want
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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 
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Ginge
Sponge Brain
Registered: 07/08/18
Posts: 202
Loc: Rocky Mountains
Last seen: 5 years, 2 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#25488795 - 09/25/18 06:10 PM (5 years, 6 months ago) |
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Pasty plate, from germination to sporulation! Sorry the date got erased by alcohol, but it's GT variety from late July or early August.
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Ginge
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Time Tourist
curioser and curioser
Registered: 01/02/16
Posts: 101
Last seen: 1 month, 4 days
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Re: Pastywhyte's Easy Agar Tek [Re: Ginge]
#25490087 - 09/26/18 09:12 AM (5 years, 6 months ago) |
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Do you need to let the agar cool down after pouring it into the plates?
Also a genreal question, is it ok to leave the plates in the pc for 24 hours (or more) after pcing? And does this also go for brf jars? Normally I don't have the time to inoculate first thing in the morning so it's usually almost 24 hours after pcing.
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,654
Loc: Exact Center
Last seen: 1 day, 11 hours
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Letting them set before closing them helps with condensation but is unnecessary, i typically leave pasty plates in the pc until i need the pc or the plates.
I wpuldnt try it with brf jars as it seems sketchy unless you are using grain jar lids and agr
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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
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Time Tourist
curioser and curioser
Registered: 01/02/16
Posts: 101
Last seen: 1 month, 4 days
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Thanks, very helpful.
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DreamReality
MycoPilot
Registered: 03/31/09
Posts: 477
Last seen: 5 months, 28 days
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I pretty much leave everything in the pc until I'm ready to use it.
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Grimsweeper
don't fear the sweeper
Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 1 month, 21 days
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If you take them out while they're still warm/hot it's easier to get them level. I usually put them on a shelf while still melted.
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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps
  
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DreamReality
MycoPilot
Registered: 03/31/09
Posts: 477
Last seen: 5 months, 28 days
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Re: Pastywhyte's Easy Agar Tek [Re: Grimsweeper]
#25493358 - 09/27/18 03:09 PM (5 years, 6 months ago) |
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Quote:
Grimsweeper said:
If you take them out while they're still warm/hot it's easier to get them level. I usually put them on a shelf while still melted.
 I'm lazy and use no pours for spores or stage 1 transfers so I don't mind them being a bit imperfect
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,654
Loc: Exact Center
Last seen: 1 day, 11 hours
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if they arent floating its a non issue
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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
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Dayglo Joe
Registered: 08/15/18
Posts: 101
Last seen: 2 years, 6 months
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Just poured my first sleeve!
Well, most of a sleeve...
I was a little heavy handed,
I learned a bunch of other things too
Things that I really didnt consider until...
...hindsight, and all...
My biggest problem is clumsy plate handling.
All thumbs!
Very comedic!
Even managed to drop a few open into my lap and onto the floor.
Ill noc that shit up anyway
Lol
Practice xfering away from contams...
Also after much excessive bare-handed juggling, outside of SAB, it crossed my mind that maybe I should "sleeve" and "de-sleeve" in the SAB with gloves and propper sterile procedures?
Im sure thats been discussed
But I somehow missed it
And thought id mention it here.
...just one of the things that crossed my mind while watching my dishes roll around my floor...
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LtLurker
Lost Sailor
Registered: 01/03/18
Posts: 7,535
Loc: Borderlands 
Last seen: 1 month, 13 hours
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Re: Pastywhyte's Easy Agar Tek [Re: Dayglo Joe]
#25506920 - 10/02/18 05:27 PM (5 years, 6 months ago) |
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If they opened when you dropped them, they're probably too contaminated to try and get a transfer from.
I unsleeve my dishes in the SAB. Easy enough to do.
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Dayglo Joe
Registered: 08/15/18
Posts: 101
Last seen: 2 years, 6 months
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Re: Pastywhyte's Easy Agar Tek [Re: LtLurker]
#25507174 - 10/02/18 06:45 PM (5 years, 6 months ago) |
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Oops.
Sorry.
Wrong thread.
As I said, ALL thumbs 
But yeah Lt, I belive your right
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Caster
Stranger
Registered: 04/17/18
Posts: 534
Last seen: 2 hours, 16 minutes
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This is great! Going to try this with some oyster mushrooms.
EDIT: one little thing I am confused about - the Agar Agar which I can buy is all powdered form, does this matter?
Edited by Pastywhyte (10/03/18 05:59 AM)
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 Buck513
Registered: 04/17/14
Posts: 5,682
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Re: Pasty Agar Tek [Re: Caster]
#25508023 - 10/03/18 01:29 AM (5 years, 6 months ago) |
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Quote:
Caster said:
the Agar Agar which I can buy is all powdered form, does this matter?
No it doesn’t. I just recently switched to the Telephone brand agar agar and I fucking love it! It’s a lot better than the stuff I was previously using.
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Fail to plan and you plan to fail. Enter the Ban Lottery
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Caster
Stranger
Registered: 04/17/18
Posts: 534
Last seen: 2 hours, 16 minutes
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Re: Pasty Agar Tek [Re: Buck513]
#25508034 - 10/03/18 01:51 AM (5 years, 6 months ago) |
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Quote:
Buck513 said:
Quote:
Caster said:
the Agar Agar which I can buy is all powdered form, does this matter?
No it doesn’t. I just recently switched to the Telephone brand agar agar and I fucking love it! It’s a lot better than the stuff I was previously using.
Thanks for that, I've got some on its way now  should be fun to do when it arrives!
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ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa 
Last seen: 1 year, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: Dayglo Joe]
#25508081 - 10/03/18 03:06 AM (5 years, 6 months ago) |
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Quote:
Dayglo Joe said:
Just poured my first sleeve!
...
It crossed my mind that maybe I should "sleeve" and "de-sleeve" in the SAB with gloves and propper sterile procedures?
Im sure thats been discussed
But I somehow missed it
And thought id mention it here.
I cut the sleeve of petries open in the SAB before I open the agar bottle. I usually do 30, which fits in my SAB snugly. I desleeve, open bottle halfway, split 10 stack into 5 stacks, open and pour bottom to top. when the agars finished, I pull the bottle out, wait 2-5 mins, re-apply alcohol gel to hands (sometimes I pour so hot my gloves get warm and dry, and cooling them down a touch helps comfort-wise) and pour the next stack.
For no Pour I just pop em in the SAB after cooking, and nocc up asap, with wedges, spore solution, or LC I'm testing.
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My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Pastywhyte 
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Re: Pasty Agar Tek [Re: Caster]
#25508239 - 10/03/18 06:00 AM (5 years, 6 months ago) |
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Quote:
Caster said:
This is great! Going to try this with some oyster mushrooms.
EDIT: one little thing I am confused about - the Agar Agar which I can buy is all powdered form, does this matter?
If you read the op I mention that you can use powdered agar and I even give measurements in volume for powdered agar to help those who lack a scale. Cheers 
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,654
Loc: Exact Center
Last seen: 1 day, 11 hours
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Powder is what i use. 2g/100ml
--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
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