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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Caps McGee]
    #25569314 - 10/26/18 01:44 PM (6 years, 2 months ago)

Guys so the first bag i started a week earlier then second, very top is still not colonized but i already see stem of mushroom on the side trying to grow/fruit. Bag is 6 weeks into it colonization. I'm assuming let it fully colonize.

Would i bury this mushroom stem in substrate one the top fully colonizes?

I'm assuming mushroom will brake the bag at come point maybe even before the bag is fully colonized.

My second bag (4 weeks in) needs more time too and also mycelium is sort of thin, not very fluffy thick white. Sort of transparent white. Thank you kindly for advice.

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InvisibleCaps McGee
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25569407 - 10/26/18 02:33 PM (6 years, 2 months ago)

Fruiting prior to full colonization is indicative of contamination... if it's a proper bag, mush won't tear it... second one sounds like a weak culture, could be genetics or contamination as well... pictures are worth more than words


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Caps McGee]
    #25569525 - 10/26/18 03:13 PM (6 years, 2 months ago)

Thank you. Here are the pics. The first pic has the stem growing. Let me know what think of it.










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InvisibleCaps McGee
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25569565 - 10/26/18 03:31 PM (6 years, 2 months ago)

They're bacterial and stress fruiting


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Caps McGee]
    #25569598 - 10/26/18 03:47 PM (6 years, 2 months ago)

Quote:

Caps McGee said:
They're bacterial and stress fruiting




What should be my course of action then and now?

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InvisibleCaps McGee
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25569607 - 10/26/18 03:50 PM (6 years, 2 months ago)

You could try to spawn it... you  might get fruit, but you could also end up losing more time and resources ... either way,  I'd start something fresh and prep your grain a good bit dryer


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Caps McGee]
    #25569632 - 10/26/18 04:04 PM (6 years, 2 months ago)

Many thanks. Should i wait until top is fully colonized before braking the cake and bulk box spanning? Or perhaps spawn in the bag?

I'm going to be doing 32qt box and will pasteurize coir in the cooler. Will coir help in keeping bacteria in check? I need some fruit out of this investment. It's really not about money but time to be honest.

It's 6 weeks in and it's not done colonizing yet. Looks like it will need another 2 weeks, 8 total plus whatever it takes for fruiting.

It would be good for me to understand what went wrong, otherwise i will be hard for me to learn from whatever happened, which i don't know what really went wrong?


Edited by Rango (10/26/18 04:32 PM)

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InvisibleCaps McGee
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25571606 - 10/27/18 02:02 PM (6 years, 2 months ago)

That top will probably not colonize... I'd try to remove the majority of it bc it's likely harboring bacteria... I think you loaded the grain too wet ... I'd start something else,  and not count on this TBH...  I doubt it does much,  if anything ... spawn should colonize within 2 weeks IMO/E


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Caps McGee]
    #25574025 - 10/28/18 01:43 PM (6 years, 2 months ago)

I bought those bags not made them myself so no noob error here from me. So if they were prepared incorrectly it was a supplier that did not properly did them.

Here is few more pics. This is the other bag that has top fully colonized but bottom is not as it was compacted and there was not a log of gas exchanges. So i'm flipping the jar (a bag) so do speak and let the gasses exchange.

Both bags are 85% colonized with mycelium. At 3rd week i had i grain colonized so it's very very slow but's it's colonizing. Perhaps strain was weak or my 80-90 temps slowed it down a bit.

Mostly i had 80-90F temp and now temps dropped to 70-80F so perhaps that triggered pinning as i read here that temp drops trigger pinning.
I'm seeing more and more pins now but they're very small.

I will spawn this with coir in box regardless of outcome to learn and get few fruits so i'm not tossing this. One it will help me learn, two until i see green mold or other sign sign of contamination i have no proof it is, just speculation which is NOT enough to take any adverse action and abort. Logical action IMO.

Question becomes do i let it fully colonize, maybe wait for that second bag pictured here for bottom to colonize then pull it or pull it now and get rid of un-colonized grain but that will mean less spawn. I'm short on spawn by 1-2qts as is, meaning with full colonization i will still be short which i'm ok with as yield will be what it will be and i'm ok with that.

Thing i'm wondering here is if 85% myclium is seen it's winning so why not let it fight it's battle? In nature environment is way more dirty. I'm not seeing any contamination. Just pure white healthy myclium. Just it's painfully slow growth. 

This is other bag where top is fully colonized but bottom is not yet. I just flipped it to the side and exposed to ambient light / fluorescent 6500k bulb too to direct myclium toward the light. I'm not sure on this either lol. But uncolonized part was in complete darkness as it was on bottom, hence my logic.

I will be using BOD's Easy AF UnBODified Monotub TEK (No Holes No Polyfil) Tek
so do i bury those pins in coir substrate when i break up this break into smaller grains and mix them with coir evenly? I'm not sure what to do with those pins, bury them in coir along with broken up cake?

Thoughts?
This is second bag with bottom still to colonize but but top fully colinized




This is first bag with top still to colonize but bottom is fully colonized.

Edited by Rango (10/28/18 03:18 PM)

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OfflineNoisecontrol
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25574324 - 10/28/18 04:06 PM (6 years, 2 months ago)

:horrified:


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Noisecontrol]
    #25574714 - 10/28/18 07:15 PM (6 years, 2 months ago)

Quote:

Noisecontrol said:
:horrified:




Hahha im not sure i understand ur reply

Care to elaborate lol

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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25574761 - 10/28/18 07:34 PM (6 years, 2 months ago)










That's not good on many levels g. Whats the rest of the bag look like n how old is it?


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Noisecontrol]
    #25574791 - 10/28/18 07:46 PM (6 years, 2 months ago)

This one is 6 weeks sir. Other one 8 weeks. Its rye juice that drained to very bottom with gravity.

Isn't that normal from cooking it and then moisture inside the bag causing this? Should i drain this with needle and siringe?

The other bag is fully colonized on bottom but top looks dry.

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OfflineNoisecontrol
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25575060 - 10/28/18 09:53 PM (6 years, 2 months ago)

Rye doesn't emit "juice" that's hardcore bacteria and i dont even know what. Now granted, a certain level of external moisture is to be expected with that much myc running but nothing like that. It definitely should not be taking 6 weeks to colonize to that point either bud. That's why it's fruiting, it's been battling for the grain. This leads me to conclude it is caused by one or more of these variables:

1. Cleanliness needs improved.
Either sterilization wasn't long enough or your inoculation procedure was dirty

2. Grain was way too wet
Which can lead to this much bacteria after that much time elapsed

3. Temps were too high
Can easily favor any bacteria present and cause it to prosper

Does any of this match your situation?

Also, what was your method of inoculation?


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Noisecontrol]
    #25575296 - 10/28/18 11:38 PM (6 years, 2 months ago)

From the looks of it now it seems that grain was too wet. It looked dry AF when i got the bags unless inside was wet and not visible from outside.

It's also possible the syringe culture was already contaminated so when injected it contaminated the rye. I've had slow growth from day 1.

The procedure i posted in first post but i follow instructions so doubt it was the procedure. It's pretty straight forward.

Ok so what's the rescue plan here. The first cake is dry and hard as rock but top still has not covered with myclelium. That's the one that's fruiting and almost 2 week older then second cake. I think this one is ready i think?

Second cake when i touch the bottom is wet. Top and sides are good. This one is not ready as i suspect inside is still wet. 

Is there way to dry this wetness like on heating unit or something?

Since myc is winning since 85% is colonized it should win yes?

Lets say i take good parts and cut out bad parts and spread it in COCO COIR.
COCO is antimicrobial so bacteria will NOT grow in there and mycelium will thrive in there. Problem solved no???

What do i do with those mush pins, bury them in coco coir. They should just break threw the sub anyway as that's what their job is yes?

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OfflineNoisecontrol
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25575348 - 10/29/18 12:07 AM (6 years, 2 months ago)

Coir is more resistant to molds due to a high lignin content, it is by no means antimicrobial. If it were we couldn't use it.

If there's bacteria at the bottom chances are the already colonized parts have bacteria throughout it as well, so don't expect spawning what is "seemingly" good myc out of those bags to another sub to get you out of the rough.

There is no way to fix what is already there. That's why we put so much diligence into the proper procedures in the first place, once it's fucked it's fucked and you're looking into save your ass techniques if and where possible.

Your best bet in trying to salavage this is separate the colonized parts from the heavily bacterial soupy parts and fruit as a cake. Going to be difficult at this point, you're gonna have to carve into the myc.

..& pray for it lol

Overall I recommend starting fresh and prepare your own stuff, that way you begin learning how to do it right and what it looks like when everything is as it should be, firsthand.


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Noisecontrol]
    #25576100 - 10/29/18 10:29 AM (6 years, 2 months ago)

Thank you for clearing this up for me.

I'm in disbelief that both bags are garbage.

I will not use another bag. I will stick with jars. More expensive but ur not losing entire material. I will need to save 4g dry out of this thing.

I'm still thinking about cutting out bad stuff and putting this in the coir and let it play out. Let them battle.

Can you think of any links for spanning this as cake?

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OfflineNoisecontrol
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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25576413 - 10/29/18 01:22 PM (6 years, 2 months ago)

Spawning and fruiting as a cake are different. If you spawn that to coir you're going to spread the bacteria again. Just cut the bottom off and fruit the cake. Hell you could even fruit from the bag like it is since  it's already begun. That heavy of bacterial presence means it wouldn't last long either way


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OfflineRango
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Re: Noob first inoculation. Is this mycelium ? [Re: Noisecontrol]
    #25576479 - 10/29/18 01:56 PM (6 years, 2 months ago)

Thank you. Any links to the easy dummy proof tek to fruit the cake?

I want to take this out of the bag and cut off the dry part out if it didn't colonize by now (8 wks).

I will start over with jars. I just need at the minimum 5g dry out of this cake which should be easy to accomplish.

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Re: Noob first inoculation. Is this mycelium ? [Re: Rango]
    #25577838 - 10/30/18 01:20 AM (6 years, 2 months ago)

Throw it in a SGFC with some foil underneath. Use the search bar to find how to build and run one, sgfc is the most common thing to use for new cultivators. 5g is probable being how much is already colonized however not guaranteed.


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