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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offlineflyskimmah
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Registered: 04/04/04
Posts: 25
Last seen: 6 years, 8 months
Sterilization question
    #2546700 - 04/10/04 02:33 PM (12 years, 7 months ago)

Different cultivation techniques call for different temps for steriliztion if using a pressure cooker. I'll be using 1/2 pint jars. One tek says 12 psi for 20 minutes, another says 15 psi for 45-60 minutes. Which is the way to go?


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Offlineflyskimmah
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Registered: 04/04/04
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Re: Sterilization question [Re: flyskimmah]
    #2546708 - 04/10/04 02:35 PM (12 years, 7 months ago)

Also meant to ask, where should I fill the water up to? Half way up the jars? A third? No water at all? Thanks for your help!


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Offlinesunshine
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Registered: 04/03/04
Posts: 36,018
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Re: Sterilization question [Re: flyskimmah]
    #2546722 - 04/10/04 02:41 PM (12 years, 7 months ago)

I'm not hte guy to ask.


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Offlinedaywalker
Do you dream in color?

Registered: 10/21/03
Posts: 783
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Re: Sterilization question [Re: flyskimmah]
    #2546737 - 04/10/04 02:45 PM (12 years, 7 months ago)

Go 45 minutes @ 15 psi for PF jars.

READ the manual with your cooker! You will need water in there to generate steam to create pressure.... READ the manual.

I use about 1"-1.5" of water in my cooker (12 qt.)

Good luck.


--------------------
"I suggest we ... learn to love our ... selves before it ... becomes illegal."


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OfflineMinimumEfrt2
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Registered: 03/17/04
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Re: Sterilization question [Re: sunshine]
    #2546797 - 04/10/04 07:00 PM (12 years, 7 months ago)

Quote:

sunshine said:
I'm not hte guy to ask.



hahahhaahahhahahah lolololololololol nice post, it cracked me up


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InvisibleMykey
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Registered: 04/07/04
Posts: 542
Re: Sterilization question [Re: flyskimmah]
    #2547512 - 04/11/04 12:41 AM (12 years, 7 months ago)

I assume that since are wandering which pressure and how long to cook,you are using a pc with a pressure guage.
The times will vary with different pressure settings due to the difference in temp in the pc.(steam pressure and temp are directly related..in other words as the steam pressure goes up the temp will too.) I am fairly sure that 15/psi corresponds to around 250 deg. This is widely accepted as the optimum for sterilizing substrate. So if you can you should definately go with this setting on your pc. You should let it go for at least 45 min, but an hour would be best. If you have to go with less pressure your temp will be lower,hence the longer it will take to ensure adequate sterilization. The 20min at 12psi you mentioned would not be enough to completely kill contams.
If you are forced to go with this setting (pc has no guage and specifies 12lb as it's pressure.)-go for about 90 min.

DEFINATELY READ YOUR MANUAL FOR OPERATING THE PC-you have to use water to generate steam and do its thing. Most of the time adding water to about a third of the way up the jar will be plenty. Too little water and it will evaporate and pressure goes down------/too much and water gets into your substrate.

Oh yeah ,dont start timing until the little weight starts to wobble a little. Hope this helps.


--------------------


Edited by Mykey (04/11/04 02:57 AM)


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Offlineflyskimmah
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Re: Sterilization question [Re: Mykey]
    #2547659 - 04/11/04 01:40 AM (12 years, 7 months ago)

Thanks very much. Another question, this one related to incubation. Again, I got competing information from the different teks. One says that after innoculation, the jars should be kept around 70 degrees but that lower temps were not a problem. Another says that they must be kept between 75-85. Which is correct? If they need to be kept warm, what's the best way to do it?


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InvisibleMykey
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Registered: 04/07/04
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Re: Sterilization question [Re: flyskimmah]
    #2547793 - 04/11/04 02:17 AM (12 years, 7 months ago)

You are welcome. Incubation in the 70's will take forever,but it will work. The optimum temp for fast colonization is as close to 86 as possible without going over. They mycilium wont die until around 106 but once you go over 86 things will really slow down and maybe stall all together. You definately want to get to 100% colonization as quickly as possible because the cake is more vulnerable to contamination while the substrate is uncolonized.
As for the incubator a friend of mine has found that a cheap cooler works quite well. A submersible fish tank heater put into a container of water (2-liter bottle or something like that) goes into the cooler with the jars to provide a consistant source of heat. You just have to play around with the settings on the heater to figure out when the temp is just right. A cheap little fishtank thermometer will work fine for monitoring the temp in the incubator. Once you get it to level out at around 84-85 air temp you should be in good shape.( the mycilium will generate the extra degree or two within the jar as it grows and devours the substrate.)


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InvisibleMagashM
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Re: Sterilization question [Re: flyskimmah]
    #2548036 - 04/11/04 04:14 AM (12 years, 7 months ago)

Grow Log
Tek : PF
Strain : South American [SA]
Spores : Came from homemade prints. 36 total jars to be injected.
Substrate :Brown Rice Flour [BRF] and Vermiculite [Verm]
---------------------------------------------------------------------------------------------------
10:15 PM 3/4/03 : Project is started. Jar prep is just putting small holes in the lids for the spore syringe and then taping them closed until inoculation. Tape used is the standard black electrical tape. First batch of jars is mixed. The mix used is 2 parts verm, 1 part water, and 1 part brf. [ This mix is the max fruiting formula by PF.] Mix is put into 1/2 pint canning jars lightly, not packed in, and left it airy. Put substrate in until it reaches the threads of the jar, then add dry verm the rest of the way.
Jars are PCd at 10psi or 240f for a period of 45 minutes. Then jars were left to sit overnight to cool.

<a href="https://files.shroomery.org/files/040103-23/23611-pic_1.jpg" target="_blank"></a>

8:39 PM 3/5/2003 : First batch of jars injected. (24 jars)

10:04 AM 3/6/2003 : Incubator is running to low 77F. Going to turn it up today.

<a href="https://files.shroomery.org/files/040103-23/69842-Pic_2_.jpg" target="_blank"></a>

5:45 PM 3/6/2003 : Second batch of jars is in the PC will be injected tomorrow. Turned up incubator runs at 83F. 86F is the best and if the incubator is 83F and myc growing in the jars is suppose to create a little heat I figure this should work out well.

1:11 PM 3/7/2003 : Second batch of jars is done. (12 jars)

12:10 PM 3/9/2003 : Jars are doing good, no signs of contams

<a href="https://files.shroomery.org/files/040103-23/74102-Pic_3_.jpg" target="_blank"></a>

2:54 AM 3/19/2003 : Tape removed from the holes today and coffee filters put over the top of the jars for added protection. Holes were opened so the CO2 in the jars could escape. I gave 12 jars to a friend who kept fucking up. 24 jar?s about 75%done.

<a href="https://files.shroomery.org/files/040103-23/89166-Pic_4.jpg" target="_blank"></a>

12:13 PM 3/22/2003 : Well it?s Saturday and the jars will probably be done by the 25th. So it was 36 jars. 12 were given to a friend, 24 done by Tuesday.

10:26 PM 3/23/2003 : 15 jars were done enough to put into dunk. I dunk and cold shock before birthing and after every flush. The other 9 jars have a spot the size of a pea that has to be covered so I?ll do those on the 25th.

<a href="https://files.shroomery.org/files/040103-23/81008-pic_5.jpg" target="_blank"></a>

7:17 PM 3/24/2003 : 15 jars put into the pod. Put in and put a layer of moist verm on top. With geolite I just put the cakes on it. With some other chambers I put the cake on a lid filled with verm. This is done to give the shrooms on the bottom and the top of the cake some added moisture.
The other 9 jars just finished so I?m going to give them a couple more days to make sure the middle of the cake is done.

<a href="https://files.shroomery.org/files/040103-23/68648-Pic_6.jpg" target="_blank"></a>

4:57 PM 3/27/2003 : 5 More cakes birthed today, 4 left going to do them tomorrow or 3/29/2003

3:58 PM 3/29/2003 : Well I lost another cake. My wife?s little 1 cake pod made from a 2 liter and a small fish pump and a little geolite used up her last cake so I gave her a SA to grow. The last 3 are in the cold shock dunk.
So the total is 36 jars done all colonized with no contams. 12 given to a friend, 1 to the wife, 23 mine

12:08 PM 3/30/2003 : Last of jars put in

12:02 PM 3/31/2003 : The first 15 cakes are pinning nicely and some have shrooms coming from the bottom.

<a href="https://files.shroomery.org/files/040103-23/38858-Pic_7.jpg" target="_blank"></a>

11:01 AM 4/3/2003 : Things look to be going well.

<a href="https://files.shroomery.org/files/040103-23/55988-Pic_8_.jpg" target="_blank"></a>

1:02 AM 4/4/2003 : Took the first flush from four of the cakes. More will be done later today.

<a href="https://files.shroomery.org/files/040103-23/36336-Pic_9.jpg" target="_blank"></a>

5/8/2003 : Total 23 cakes 164 grams dried.


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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