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OfflineObsidian
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Why should I aim for rhizomorphic myc? * 1
    #25466394 - 09/17/18 07:42 AM (2 years, 7 months ago)

I've been playing with agar for some time and have been quite successful in getting nice clean cultures even from contaminated plates. However I just don't get any rhizomorphic mycelium and I'm sort of frustrated with that. It's all nice and fuzzy, milky white tomentose growth, but never any rhizomorphic groth.

So I was wondering - should even I be obsessing myself with rhizo or just go along with what I have?

Further, I'd like to know what is the advantage of rhizomorphic mycelium, and could there possibly be any benefits to tomentose growth (it seems to me like it is more evenly spread across the surface of the substrate and thus possibly does a better job in metabolizing it)?

What doth thee sayeth mine own valorous folks?


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InvisibleTookitooki
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Re: Why should I aim for rhizomorphic myc? [Re: Obsidian]
    #25466497 - 09/17/18 09:09 AM (2 years, 7 months ago)

Give us details of your agar media.  Nutrient composition and amounts play a role in growth appearance.    Got any pictures?


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Offlineteladi
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Re: Why should I aim for rhizomorphic myc? [Re: Tookitooki]
    #25466634 - 09/17/18 10:40 AM (2 years, 7 months ago)

Nope. Rhizo growth makes for nice photos. Some tend to exhibit rhizo, others tormentose growth. The key is in learning to identify and deal with contamination of all kinds. Don't stress how your guys are growing, only that they are healthy, happy fungi.


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Edited by teladi (09/17/18 10:42 AM)


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OfflineManifoldPrime
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Re: Why should I aim for rhizomorphic myc? [Re: teladi]
    #25466753 - 09/17/18 11:39 AM (2 years, 7 months ago)

I like ropey cultures because to me thats a few extra confirmations: I am indeed looking at cube myc and not a contaminate like oyster(its happened) or mold, The sectoring is more obvious IMO,and rhizomorphs are usually faster IMO. I like the look of them, and I've seen rhizos bridge gaps in substrate/grain.

Do lots of germ plates from your prints, isolate the ropier colonies, and isolate. I tend to prioritize ropey myc and now I have lots of different variants with selected-for rhizomorphs.

PESA and Thai var. makes some nice ropes, from personal exp. and observation. You can get it from most any var. with a good genetics roll and a bit of transferring.


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OfflineObsidian
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Re: Why should I aim for rhizomorphic myc? [Re: Tookitooki]
    #25467067 - 09/17/18 02:23 PM (2 years, 7 months ago)

I am using pure BRF/agar.

Here's a photo of one typical culture of mine (sorry for the potato quality of my phone camera).



This one is long overdue because it was actually contaminated with trich but I used it to isolate clean culture (cut-spots are somewhat visible). As you can see it eventually won against molds. Now I'm keeping it to see if will contaminate (I am exposing it to open air daily).

Apart from the fact that I can't get rhizo growth I am actually very happy with my culture,  it tends to eat right through ANY contamination (wet-spots, green, black molds) I've never seen anything like that. And it comes from 10+ year old spores that I almost left for dead.

Yeah, I guess my boys are ok after all.


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InvisibleTookitooki
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Re: Why should I aim for rhizomorphic myc? [Re: Obsidian]
    #25467485 - 09/17/18 05:19 PM (2 years, 7 months ago)

Take the same culture and see how it reacts on a different nutrient agar.  Or cut your nutes in half and see how it reacts.


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OfflineJohnny.rotten
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Re: Why should I aim for rhizomorphic myc? [Re: Tookitooki]
    #25467811 - 09/17/18 07:44 PM (2 years, 7 months ago)

Quote:

Tookitooki said:
Take the same culture and see how it reacts on a different nutrient agar.  Or cut your nutes in half and see how it reacts.



:whathesaid:
As soon as i cut down the nutrients in my agar, my next transfer was rhizo.


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