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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14331849 - 04/22/11 05:55 AM (12 years, 9 months ago)

yip no worries doc, i have seen a few discussions about uvc lighting here but seems like it’s from people that are looking for alternatives to flow hoods or glove boxes or thinking of a fast way to sterilize their glove boxes.


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OfflineMycelio
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14332504 - 04/22/11 09:45 AM (12 years, 9 months ago)

Hi again,

Quote:

dr harry bush said:
i have been researching the net for clues on how this should be carried out. i have come up with a total of two experiments that are close to this one. i am basing mine closely to an experiment that was used to mutate mold.



within a minute I can find several documented procedures, for example related to creating sporeless mutants in the genus pleurotus. I'm sure there is also something with P. cubensis. Perhaps you just try different search terms.


Quote:

dr harry bush said:
i was hoping to have the info here so others could learn from it and i prefer practical than just reading the theory.



Sure, that is nice, but reading will give you important hints, which you won't find here.


Quote:

dr harry bush said:
if you read the first post i wanted to see how many minutes of uvc it takes to kill mycelium and mutants would be a bonus. i do know that there is greater chance that nothing will happen and that the mycelium will be repaired/replaced .... blah blah blah ...... but there is just that 1 percent chance that something many happen or we might just learn something from this and turn a myth into reality



Like Doc_T wrote, that time depends on the actual intensity of your UV-C light and the distance to the mycelium.

I also share Doc_T's concerns, please be careful and protect your skin and eyes!

Carsten


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OfflineMolecularConcept
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Re: exposing mycelium to uvc lighting [Re: Mycelio]
    #14335505 - 04/22/11 09:23 PM (12 years, 9 months ago)

I'm about to do some digging for some papers on on this if anyone finds one of particular interest how about sharing.

I knew UVC could cause burns and blindness. I did not know it could do so that quickly!  Holy crap! So thanks for that warning.

  Keep us updated. I know a there are a few people here interested in mutations


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InvisibleDoc_T
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Re: exposing mycelium to uvc lighting [Re: MolecularConcept]
    #14336348 - 04/23/11 12:20 AM (12 years, 9 months ago)

Quote:

MolecularConcept said:
I know a there are a few people here interested in mutations




I hope these people will remember that UV light will cause mutations in them.


Not the cool kind, either. :nonono:


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Offlineshopdropper
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Re: exposing mycelium to uvc lighting [Re: Doc_T]
    #14336944 - 04/23/11 03:03 AM (12 years, 9 months ago)

lets hope so!

So do the exposures in a covered area and turn light on remotely!  Otherwise cover exposed skin and wear a really good pair of shades.


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DON'T TRY THIS AT HOME: no guarantees can be made about the accuracy of the information herein. The information dicussed in these posts is purely hypothetical, and for intelectual purposes only. Any similarity between internet chat and real life is pure coincidence.

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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: shopdropper]
    #14339049 - 04/23/11 03:29 PM (12 years, 9 months ago)

Thanks Mycelio iv been looking into a bit more information on the net.

Just an update on this experiment. All the petris where exposed to the same amount of light again while the petri lids where removed. +- 24 hours the dishes where checked under black light to check growth. There has been new growth on all on all the dishes the pertri at one hour shows good growth but much less than the petris exposed to minimum uvc times. The petri that was exposed to uvc for 5 minutes is showing the best growth. I will be taking pictures of the petris tomorrow evening and will post them to show you the growth.


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14343655 - 04/24/11 02:06 PM (12 years, 9 months ago)

here are the updated pictures of growth. these dishes are being held above my blacklight bulb.



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OfflineAmphiprion
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14343692 - 04/24/11 02:15 PM (12 years, 9 months ago)

Awesome Work DR, This is an amazing experiment :smile:


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Amphi


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: Amphiprion]
    #14596477 - 06/11/11 02:59 PM (12 years, 8 months ago)

ok just an update on the progress of this grow. i currently have tk uvc 5 min in 6 jars of grain. i should be spawning this to ccv in about 7 days.

i will post pictures very soon.

peace


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Offlinedr harry bush
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14761737 - 07/13/11 10:56 AM (12 years, 7 months ago)

good evening everyone

here is a little update on the progress of this experiment.
i spawned my grain to 5% coffee, 10% gypsum, 45% coir, 40% verm. i sealed up the mono for 3 days then exposed it to fruiting conditions, 7 days later i had pins and this is the 3rd day into fruiting.

i have not noticed any mutant as yet.

i will print a few of theses and grow them to see if there are any mutants down the line.



peace


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OfflineToofpaste
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #14762875 - 07/13/11 02:19 PM (12 years, 7 months ago)

So stringy... What strain?


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OfflineFeelers
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Re: exposing mycelium to uvc lighting [Re: Toofpaste]
    #14765219 - 07/13/11 10:14 PM (12 years, 7 months ago)

High CO2?

Looks like you're in need of even higher exposure. Good work on the experiment btw! :thumbup:


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OfflineSagedrake690
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #25465705 - 09/16/18 08:55 PM (5 years, 4 months ago)

Well wouldnt this help to keep trich and other contamination from happening? Or at least prevent it to keep your cakes longer? Im experimenting with perpetual colonies. Keeping them alive and I was curious on how well they faired against the light. I know they use these to completely sanitize everything so Im curous to see if this will keep a colony healthy past a few fruitings.


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InvisiblebodhisattaMDiscordReddit
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Re: exposing mycelium to uvc lighting [Re: dr harry bush]
    #25466360 - 09/16/18 08:55 PM (5 years, 4 months ago)

This thread has been closed.

Reason:
7 years old and no it would probably help trich. As uvc doesn't do much but damage mycelium the faster growing species like trich would likely have an easier time and run more rampantly over your cubes.


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