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OfflineJavichunte
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Semi-professional shiitake cultivation
    #25464139 - 09/16/18 10:21 AM (5 years, 6 months ago)

Hello everyone and greetings from Spain, I communicate through a translator, I will try to translate as best as possible, I just established a shiitake cultivation company, soon show images of the facilities,I will show all my advances and doubts. Right now I am making bags of spawn, but the mycelium becomes very thin, the bags are 100% colonized but they are hardly appreciated, why? Thanks in advance



Edited by Javichunte (09/20/18 03:41 AM)

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Offlinefrog48
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Re: Semi-professional shiitake cultivation [Re: Javichunte]
    #25464309 - 09/16/18 11:53 AM (5 years, 6 months ago)

how long have they been colonizing?

:frenchie:

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: Cultivo de shiitake semiprofesional</font></font> [Re: frog48]
    #25464329 - 09/16/18 12:00 PM (5 years, 6 months ago)

Quote:

frog48 said:
how long have they been colonizing

:frenchie:



Two weeks and 4 days, It contains 1.5kg of wheat

Edited by Javichunte (09/16/18 12:12 PM)

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: Cultivo de shiitake semiprofesional</font></font> [Re: Javichunte]
    #25464641 - 09/16/18 01:46 PM (5 years, 6 months ago)

Then you might have either a mold or à bacterial problem. It should be much whiter after two weeks.

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;" Re: Cultivo de shiitake semiprofesional </ font> </ font [Re: frog48]
    #25464792 - 09/16/18 02:36 PM (5 years, 6 months ago)

Quote:

frog48 said:
Then you might have either a mold or à bacterial problem. It should be much whiter after two weeks.



But it has no evidence of bacteria or mold in sight

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;" Re: Cultivo de shiitake semiprofesional </ font> </ font [Re: Javichunte]
    #25464950 - 09/16/18 04:00 PM (5 years, 6 months ago)

What did you inoculate with and how much?


--------------------

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25466005 - 09/16/18 11:49 PM (5 years, 6 months ago)

Quote:

Quadman said:
What did you inoculate with and how much?



Transfer g2g with a tablespoon of soup per bag, from mycelium purchased from mycelia, strain 3790

Edited by Javichunte (09/19/18 05:44 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466038 - 09/17/18 12:25 AM (5 years, 6 months ago)

The wheat was cooked for about 15 minutes, until it was cooked properly, then it was allowed to dry until it did not get its hands wet when it was touched and put it in vacuum-sealed bags.

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OfflineFungalDane
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466126 - 09/17/18 01:27 AM (5 years, 6 months ago)

is there a filterpatch on the bags? Since you mention vacuum sealed..

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: FungalDane]
    #25466198 - 09/17/18 02:08 AM (5 years, 6 months ago)

Quote:

FungalDane said:
is there a filterpatch on the bags? Since you mention vacuum sealed..



If pp bags with 2micron filter

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466203 - 09/17/18 02:11 AM (5 years, 6 months ago)

I have to mention that the sterilization was not good, eliminate several bags with bacteria but these balls are apparently clean

Edited by Javichunte (09/17/18 02:12 AM)

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Offlinedrake89
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Trusted Cultivator
Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466313 - 09/17/18 04:28 AM (5 years, 6 months ago)

You can have bacteria without visual evidence.  What is the smell?  1 TBSP per bag is not very much...  smell should be similar to fresh shiitake mushrooms


--------------------
Fiery Fungi (like us on faeboo)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: drake89]
    #25466334 - 09/17/18 04:50 AM (5 years, 6 months ago)

Quote:

drake89 said:
You can have bacteria without visual evidence.  What is the smell?  1 TBSP per bag is not very much...  smell should be similar to fresh shiitake mushrooms



Thank you, I did not smell, I know the smell of mycelium and grain with bacteria, this afternoon I'll put my nose to work. Should I put 2 tablespoons? Or what percentage?

Edited by Javichunte (09/17/18 05:57 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466529 - 09/17/18 07:39 AM (5 years, 6 months ago)

I can not smell anything after the filter, I put pictures today. Would you use this spawn to inoculate the final substrate, if when you open the bags you smell of pure mycelium?



Edited by Javichunte (09/17/18 08:04 AM)

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;" Re: Cultivo de shiitake semiprofesional </ font> </ font [Re: Javichunte]
    #25466630 - 09/17/18 08:38 AM (5 years, 6 months ago)

Quote:

Javichunte said:
I have to mention that the sterilization was not good, eliminate several bags with bacteria but these balls are apparently clean



Did you sterilize for at least 90 minutes at 15 psi?
Quote:

Javichunte said:
Quote:

frog48 said:
Then you might have either a mold or à bacterial problem. It should be much whiter after two weeks.



But it has no evidence of bacteria or mold in sight



Time it takes to consolidate and incomplete colonization are signs of contaminates.


--------------------

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25466652 - 09/17/18 08:52 AM (5 years, 6 months ago)

Quote:

Quadman said:
Quote:

Javichunte said:
I have to mention that the sterilization was not good, eliminate several bags with bacteria but these balls are apparently clean



Did you sterilize for at least 90 minutes at 15 psi?
Quote:

Javichunte said:
Quote:

frog48 said:
Then you might have either a mold or à bacterial problem. It should be much whiter after two weeks.



But it has no evidence of bacteria or mold in sight



Time it takes to consolidate and incomplete colonization are signs of contaminates.



Agree but colonization if it is complete and time 20 days to consolidate

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InvisibleInocybe
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25466707 - 09/17/18 09:19 AM (5 years, 6 months ago)

I think It's ok. Shiitake myc is thin at first, give it some days and see how it develops.

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Inocybe]
    #25466739 - 09/17/18 09:34 AM (5 years, 6 months ago)

Quote:

Inocybe said:
I think It's ok. Shiitake myc is thin at first, give it some days and see how it develops.



But only in a bag, look how well it colonizes

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25467386 - 09/17/18 02:37 PM (5 years, 6 months ago)

That's happy myc, much better than the other bags
What's the différence ?

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OfflineJdp shroom
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25468001 - 09/17/18 06:52 PM (5 years, 6 months ago)

Looks really good. Way better than the other bag

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Jdp shroom]
    #25468681 - 09/18/18 12:34 AM (5 years, 6 months ago)

Well, the only difference that there is is the filtering system, this system is the one of the last bag, the correct bag


Edited by Javichunte (09/18/18 01:33 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25468698 - 09/18/18 12:52 AM (5 years, 6 months ago)

The other bags have this filtration system with 2 microns. Could I filter too much for shiitake mycelium?


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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25469531 - 09/18/18 10:40 AM (5 years, 6 months ago)

No the filter should be good. I'm using 5 micron bags.


--------------------

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25469909 - 09/18/18 01:01 PM (5 years, 6 months ago)

Did you buy a mother strain from mycelia or a bag of grain spawn?  My best guess is contamination in G2G transfer. Either in flow hood or due to insufficient sterilization of grain.
:frenchie:

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25469964 - 09/18/18 01:23 PM (5 years, 6 months ago)

:whathesaid:


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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25471740 - 09/19/18 12:52 AM (5 years, 6 months ago)

Quote:

Quadman said:
Quote:

Javichunte said:
I have to mention that the sterilization was not good, eliminate several bags with bacteria but these balls are apparently clean



Did you sterilize for at least 90 minutes at 15 psi?
Quote:

Javichunte said:
Quote:

frog48 said:
Then you might have either a mold or à bacterial problem. It should be much whiter after two weeks.



But it has no evidence of bacteria or mold in sight



Time it takes to consolidate and incomplete colonization are signs of contaminates.




Se esterilizó durante al menos 90 minutos a 15 psi?
Now I see your question, I sterilize at 1.5 mpa = 21.7 psi, for 2.30 hours, but contamination continues, the last sterilization went up to 0.2 mpa = 29 psi and 2.30h

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25471877 - 09/19/18 03:10 AM (5 years, 6 months ago)

Quote:

frog48 said:
Did you buy a mother strain from mycelia or a bag of grain spawn?  My best guess is contamination in G2G transfer. Either in flow hood or due to insufficient sterilization of grain.
:frenchie:



We will see it soon, but I think it is due to some other factor, such as that the humidity of the grain is not correct or that this strain colonizes in this way

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OfflineScotTony
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25471950 - 09/19/18 05:10 AM (5 years, 6 months ago)

You can not incubate wheat at temperatures higher than 22 C in spawn bags because of endospores. For jars you can go a little higher than that, max 26 C.

Also if you shake often shiitake can look like this. I believe you meant 3790. Might be okay, it does not like plain wheat so much. Try adding some sawdust to the next batch.

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: ScotTony]
    #25471995 - 09/19/18 05:50 AM (5 years, 6 months ago)

Ohh, I did not expect this answer, I liked it, it's a cold strain, I started to incubate at 23 ° C to ajilizar, today I'll put it at 22 ° C, maybe I should ask what strain is right for my farm. Correct 3790, very observant

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25472014 - 09/19/18 06:02 AM (5 years, 6 months ago)

My sachets are going to have shavings and oak serine, I do not rule out introducing some soft woods in the future, also wheat bran, calcium carbonate and calcium sulfate, here the temperatures are very cold, reaching -15 ° C in winter and very high in summer, reaching 40 ° C, humidity, "almost zero" , but what I'm looking for is quality in my mushrooms, if I see that the quality does not matter to my customers, I'm afraid not, I'll change to more productive. Thank you all for your kind comments

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InvisibleInocybe
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25472141 - 09/19/18 07:14 AM (5 years, 6 months ago)

When we talk about cold or warm weather strains, we refer to fruiting temperature, not incubation temperature. If I were you I would incubate the bags at room temperature (arround 21ºC). You also have to be careful reading "incubation temperatures" because it's not the same "room temp" than "substrate temp". The temperature inside of the bag colonizing usually is 3 to 4ºC more than the room temp, so when you read somewhere that incubation optimum is 25ºC, you should manage the room temp to be 21ºC or so, in order to mantain the 25ºC inside of the bag. You talked with me via whatsapp a month ago or so, I would like to help you but I lost my phone and I'm looking for a new one. Good luck.

Edited by Inocybe (09/19/18 07:15 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Inocybe]
    #25472184 - 09/19/18 07:39 AM (5 years, 6 months ago)

Quote:

Inocybe said:
When we talk about cold or warm weather strains, we refer to fruiting temperature, not incubation temperature. If I were you I would incubate the bags at room temperature (arround 21ºC). You also have to be careful reading "incubation temperatures" because it's not the same "room temp" than "substrate temp". The temperature inside of the bag colonizing usually is 3 to 4ºC more than the room temp, so when you read somewhere that incubation optimum is 25ºC, you should manage the room temp to be 21ºC or so, in order to mantain the 25ºC inside of the bag. You talked with me via whatsapp a month ago or so, I would like to help you but I lost my phone and I'm looking for a new one. Good luck.



Our temperature environment is now 25 ° c, but it cools to 23, I will go down to 21 as you say, I leave the data of my strain.
Are you telling me we've talked about WhatsApp? Can you tell me who it is?
http://www.mycelia.be/en/strain-list/m-3790-lentinula-edodes

Edited by Javichunte (09/19/18 07:47 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25472556 - 09/19/18 11:42 AM (5 years, 6 months ago)

Hello, it seems that they are whitening, I put a photos, also of
my facilities



internet imagenes

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25472861 - 09/19/18 01:44 PM (5 years, 6 months ago)

Very nice. I would say that those bags look ok. They were probably just slow from a low rate on your grain to grain transfer


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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25472996 - 09/19/18 03:00 PM (5 years, 6 months ago)

Hello, it seems that they are whitening, I put a photos, also of
my facilities
Quote:

Quadman said:
Very nice. I would say that those bags look ok. They were probably just slow from a low rate on your grain to grain transfer



Quote:

Quadman said:
Very nice. I would say that those bags look ok. They were probably just slow from a low rate on your grain to grain transfer



Quote:

Quadman said:
Very nice. I would say that those bags look ok. They were probably just slow from a low rate on your grain to grain transfer



Thanks, I will try to add more mycelium for post incubations, I will show it

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25474192 - 09/20/18 12:34 AM (5 years, 6 months ago)

Good morning, do you advise me to follow mycelia's specifications perfectly? It really seems low humidity for the substrate
Another thing that worries me is if you can reuse the bags, I've heard that the filter can dilate too much, yes, no, how many times?

Edited by Javichunte (09/20/18 03:47 AM)

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25476882 - 09/21/18 12:57 AM (5 years, 6 months ago)

Can anyone answer me?

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25476969 - 09/21/18 03:08 AM (5 years, 6 months ago)

Well tomorrow isolate the tissue from the same strain, I will show the process in an orderly manner

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OfflineScotTony
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25477063 - 09/21/18 05:08 AM (5 years, 6 months ago)

You can reuse the bags. The filters are usually good for reuse but the plastic is harder and you must be more careful sealing them. It must be clean for a good seal or it breaks easily. It might be easier to use new ones, buy them bulk and they will be cheap. I try to reuse when I can, because it is ecological.

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: ScotTony]
    #25477282 - 09/21/18 07:24 AM (5 years, 6 months ago)

Quote:

ScotTony said:
You can reuse the bags. The filters are usually good for reuse but the plastic is harder and you must be more careful sealing them. It must be clean for a good seal or it breaks easily. It might be easier to use new ones, buy them bulk and they will be cheap. I try to reuse when I can, because it is ecological.



I think it's a good gesture, to reuse the bags to avoid polluting, that's why we want to reuse, we have many. Thank you very much

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25482329 - 09/23/18 06:26 AM (5 years, 6 months ago)

Hello everyone, yesterday I made tissue cultures and made transfers from other cultures, to see their behavior before being used to inoculate

imagenes de link

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25482641 - 09/23/18 09:43 AM (5 years, 6 months ago)

Can someone tell me how to get the mycelium out of a test tube? It has been impossible with the scalpel

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25482653 - 09/23/18 09:50 AM (5 years, 6 months ago)

Fill your test tube fuller.:wink:

I have a small 90° bent blade


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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25484957 - 09/24/18 10:58 AM (5 years, 6 months ago)

The inclined agar of the tube, almost touches the filter, as you tell me I will make a utensil

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25485266 - 09/24/18 12:57 PM (5 years, 6 months ago)

You can make the incline steeper and have more agar in your tube. They will store for much longer that way.


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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25487019 - 09/25/18 12:25 AM (5 years, 6 months ago)

Quote:

Quadman said:
You can make the incline steeper and have more agar in your tube. They will store for much longer that way.



Hello, would you refer to less inclined ??

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OfflineQuadman
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25487902 - 09/25/18 12:02 PM (5 years, 5 months ago)

A tube only holds so much. The more you tip it over the less it holds. If you lay it completely flat it is empty.:shrug: Laying you tubes higher will make them hold more agar.


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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Quadman]
    #25489798 - 09/26/18 06:23 AM (5 years, 5 months ago)

Quote:

Quadman said:
A tube only holds so much. The more you tip it over the less it holds. If you lay it completely flat it is empty.:shrug: Laying you tubes higher will make them hold more agar.



Jajjaja okok, I did not understand well. Thank you

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25497871 - 09/29/18 11:03 AM (5 years, 5 months ago)

At the end of everything, it was correct

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25511460 - 10/04/18 10:28 AM (5 years, 5 months ago)

Hello everyone, we are already cutting branches to produce splinters.

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25511619 - 10/04/18 11:38 AM (5 years, 5 months ago)

Can someone tell me approximately, what is the profitability of shiitake, in dry serrin?

Edited by Javichunte (10/04/18 01:43 PM)

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25513275 - 10/04/18 10:10 PM (5 years, 5 months ago)

what is dry serrin?


:frenchie:

Edited by frog48 (10/04/18 10:11 PM)

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25513284 - 10/04/18 10:13 PM (5 years, 5 months ago)

Better use sawdust by the way, you'll rip open your spawn bags with these chips and the yield is not going to be much better than sawdust.

:frenchie:

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25514688 - 10/05/18 01:07 PM (5 years, 5 months ago)

Quote:

frog48 said:
Better use sawdust by the way, you'll rip open your spawn bags with these chips and the yield is not going to be much better than sawdust.
:frenchie:




I put 60% of poplar sawdust, 10% of wheat bran and 30% of these chips that are free

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25514697 - 10/05/18 01:11 PM (5 years, 5 months ago)

You can supplement up to 20 %

:frenchie:

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OfflineJavichunte
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: frog48]
    #25514765 - 10/05/18 01:30 PM (5 years, 5 months ago)

Quote:

frog48 said:
what is dry serrin?


:frenchie:



Sawdust without moistening, to do my calculations

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Offlinefrog48
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25514854 - 10/05/18 02:08 PM (5 years, 5 months ago)

I'm harvesting about 500 grams per block, wich is about 20 %  wet weight. If everything goes well, not always the case

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InvisibleTits on a bull
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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: <font style = "vertical-align: inherit;"> <font style = "vertical-align: inherit;"> Re: <font style = "vertical-align: inherit;"> <font [Re: Javichunte]
    #25518170 - 10/06/18 08:56 PM (5 years, 5 months ago)

Quote:

Javichunte said:
Can someone tell me how to get the mycelium out of a test tube? It has been impossible with the scalpel



Why couldn't you just PC some karo water, draw into a sterile needle, squirt it into test tube and draw it back up? Blasting it off and spreading the culture. I imagine you could even use a injection port cap on testtube.Easy to use, very reusable.


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Stop talking about being enlighten. Crybabies, bitches, and people worried about ratings can never reach enlightenment!  NEVER!

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