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OfflineWanman30
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Liquid culture - mycelium or not mycelium. That is the question
    #25459240 - 09/14/18 09:59 AM (2 months, 3 days ago)

I've been a member of shroomery for over a decade. First time posting a question.

I know these id requests can be a bit tedious however I'd like some advice and people's thoughts on whether this looks like mycelium?

I wanted make a spore syringe go further so went for lc despite the the pitfalls.

It was inoculated about 5/6 days ago. I know you can't tell for sure if there is a contamination but would appreciate any advice.

Thanks guys in advance. First time I've knocked up a LC.



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OfflineThe Mycologist
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: Wanman30]
    #25459246 - 09/14/18 10:03 AM (2 months, 3 days ago)

Only way to test LC is to put it on agar.


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Offlinefishermansjc
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: The Mycologist] * 1
    #25459255 - 09/14/18 10:07 AM (2 months, 3 days ago)

OR put it to a test 1/2 pint of grain.

If it is good, you can G2G that and use it. If not, toss it and start again.


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OfflineNineInchNails
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: fishermansjc]
    #25459392 - 09/14/18 11:19 AM (2 months, 2 days ago)

It's hard to tell from the pic, but does it look stringy like snot and breaks apart when you lightly swirl the LC? If so then it is not MYC.

Did your LC get foggy at all since you inoculated it? If so then that's a sign of contam. I've actually used foggy LCs before and my trays did not produce any mold.


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Offlinemushboy
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: Wanman30]
    #25459426 - 09/14/18 11:32 AM (2 months, 2 days ago)

youve been lurking for a decade but you do this??

Quote:

Wanman30 said:
I wanted make a spore syringe go further so went for lc despite the the pitfalls.





:whateveryousayfreak:

didnt you read a billion posts/flames saying how risky that is? ive never ever gotten syringed LC to grow cleanly.

ive tried way to much


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Offlinefishermansjc
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: mushboy]
    #25459453 - 09/14/18 11:47 AM (2 months, 2 days ago)

It is definitely possible. You just need good sterile technique and a nice print to start with.

I wouldn't recommend it. Put spores to a pasty no pour and do 1 transfer even to make sure you can get clean growth for the LC.

The Josex poke method would work well.


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OfflineLtLurker
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: fishermansjc]
    #25459594 - 09/14/18 12:57 PM (2 months, 2 days ago)

I'm with mushboy on this one. If the goal is to stretch out the syringe you have, it doesn't make sense to attempt spores>lc where the average fail rate is over 50%. Dirty lc attempts only make sense if it's a "fuck it, who cares if it fails" kinda situation.

If you can make LC, you can make agar. Agar would stretch the spores even further to. It'd be the best way to test and clean up what you got goin now.


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Offlinemushboy
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: LtLurker]
    #25459600 - 09/14/18 12:59 PM (2 months, 2 days ago)

:whathesaid:
ive grown insane amounts of mushrooms from just 1 drop of spores on a pasty plate.:thumbup: (i think most of us have)


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OfflineWanman30
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: mushboy]
    #25459722 - 09/14/18 02:22 PM (2 months, 2 days ago)

Pastey plate? What do you mean? If the LC don't work ill move forward with something else. I have old prints, would you suggest I use them in a syringe or another method?


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OfflineLtLurker
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: Wanman30]
    #25459733 - 09/14/18 02:27 PM (2 months, 2 days ago)

It's no-pour agar in re-usable dishes instead of petris.
https://www.shroomery.org/forums/showflat.php/Number/19208976

There's also a whole tek list stickied at the top of the forum and a search feature. You should be able to find everything you need.


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OfflineNineInchNails
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: LtLurker]
    #25459940 - 09/14/18 04:12 PM (2 months, 2 days ago)





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OfflineteladiS
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Re: Liquid culture - mycelium or not mycelium. That is the question [Re: NineInchNails]
    #25460003 - 09/14/18 04:44 PM (2 months, 2 days ago)

2 spores can make fuck tons of mycelium, here's some pointless math assuming a whole lot of them:

1. Streak spore print to agar. Do a transfer at maybe 10 mm radius. Cut out 5 mm segments on edge. 62 mm circumference yields 12 secondary plates.
2. Transfer at 20 mm radius, 'cause it doesn't look quite good. 125 mm -> 25 tertiary plates per secondary -> 300 tertiary plates
3. All 300 tertiary plates look healthy, so you grow them out a whole lot. 45 mm radius to extend to plate boundary -> 282 mm circumference. 21G needle has an outer diameter of 0.8192 mm. Let's round that up to 1 mm, so we can get 282 Josex biopsy from each tertiary plate -> 300*282 = 84'600 LC jars.
4. Let's say each LC jar is only 100 ml, that's 8.5 metric tons of broth. They get poured out to 10 grain masters each -> 846'000 grain masters
5. 200g per grain master requires 19 metric tons of grain. 4 hactares/10 acres of oats if you're growing your own. Most of that weight will be mycelium soonishly.
6. Each grain master can inoculate multiple other grain jars. How many? 5 each? That'll yield 4 million grain jars.
7. You'll end up with 114 tons of colonized grain. Spawn to bulk at a 1:1 ratio, and you will have more than 220 tons of mycelium. Everything goes right, your BE is really good, and you manage to harvest 200 wet tons of mushrooms. You dry them out, and end up with 20 dry tons.

20 dry tons of mushroom is less than 0.3% of Europe's 2015 consumption. Not all that in the bigger picture, except when you realize you harvested all of that from one spore print.


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