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Offline99.99
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Parafilm cracking on agar plate contamination risk ??
    #25436066 - 09/04/18 08:17 PM (5 years, 4 months ago)

I have a roll of parafilm I bought about four years ago now that I’m getting toward the end of it it’s starting to crack and come apart on my agar dishes just wondering if this will cause contamination on my agar


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OfflineWing
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25436483 - 09/04/18 11:17 PM (5 years, 4 months ago)

Possibly, if the part that it covering the gap between lid is cracked. Also, if its cracking I would think that the integrity could be compromised and that would be a good indication to me to get a new roll ASAP. Not worth the hassle of contam issues


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Offline99.99
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Wing]
    #25664487 - 12/07/18 08:49 PM (5 years, 1 month ago)

No matter what I did the parafilm keeps on cracking ,tried a brand new role and some stuff I have l had left over over all the same thing
I just fixed it by switching to this stuff instead I like it a lot better it’s really clear and sticks really well
Grafting Tape Graft Membrane PE Biodegradable Waterproof Gardening Bind Belt https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F273566260323


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InvisibleJHOVA
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25664766 - 12/08/18 12:34 AM (5 years, 1 month ago)

bod mentioned that the cheap shit you get from the grocery store lasts longer.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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InvisibleBumholio
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25664776 - 12/08/18 12:46 AM (5 years, 1 month ago)

Quote:

99.99 said:
No matter what I did the parafilm keeps on cracking ,tried a brand new role and some stuff I have l had left over over all the same thing
I just fixed it by switching to this stuff instead I like it a lot better it’s really clear and sticks really well
Grafting Tape Graft Membrane PE Biodegradable Waterproof Gardening Bind Belt https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F273566260323



That's what I use, I get it off Amazon though, it's really good. Bit of a PITA to get off again though but so is cling film:shrug:


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"great things may come to those who wait, but only what's left by those who hustle"


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InvisibleMycolorado
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25665045 - 12/08/18 06:33 AM (5 years, 1 month ago)

Have your plates contaminated as a result?


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Offline99.99
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Mycolorado]
    #25666589 - 12/08/18 07:58 PM (5 years, 1 month ago)

Quote:

Mycolorado said:
Have your plates contaminated as a result?




Yes around the edges went bad on the plates that the patafilm cracked


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InvisibleMycolorado
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25667254 - 12/09/18 07:18 AM (5 years, 1 month ago)

It’s pretty hard for contams to travel up under the lid and squeeze through the almost non existent space between the lid and plate and then fall on your agar.  Your technique is likely the issue.  Parafilm can split on the sharp edge of the lid after a time but shouldn’t be causing contams on your plates.  I keep plates in an old sab for an added layer of protection.  Got any pics?

This is what I see happen occasionally but I’ve never had an issue with it:



You might try plastic wrap.  People like it better than parafilm and it’s cheaper. 



Edited by Mycolorado (12/09/18 07:58 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Bumholio]
    #25667448 - 12/09/18 09:25 AM (5 years, 1 month ago)

Quote:

Bumholio said:
Quote:

99.99 said:
No matter what I did the parafilm keeps on cracking ,tried a brand new role and some stuff I have l had left over over all the same thing
I just fixed it by switching to this stuff instead I like it a lot better it’s really clear and sticks really well
Grafting Tape Graft Membrane PE Biodegradable Waterproof Gardening Bind Belt https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F273566260323



That's what I use, I get it off Amazon though, it's really good. Bit of a PITA to get off again though but so is cling film:shrug:



Cling film hard to remove? Wut


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InvisibleBumholio
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: bodhisatta]
    #25667468 - 12/09/18 09:34 AM (5 years, 1 month ago)

Quote:

bodhisatta said:
Quote:

Bumholio said:
Quote:

99.99 said:
No matter what I did the parafilm keeps on cracking ,tried a brand new role and some stuff I have l had left over over all the same thing
I just fixed it by switching to this stuff instead I like it a lot better it’s really clear and sticks really well
Grafting Tape Graft Membrane PE Biodegradable Waterproof Gardening Bind Belt https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F273566260323



That's what I use, I get it off Amazon though, it's really good. Bit of a PITA to get off again though but so is cling film:shrug:



Cling film hard to remove? Wut



I can never find the end of it.


--------------------


"great things may come to those who wait, but only what's left by those who hustle"


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InvisiblebodhisattaMDiscordReddit
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Bumholio]
    #25667480 - 12/09/18 09:37 AM (5 years, 1 month ago)

You can just pull it off from any spot. Or pull it all off intact as a ring.


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InvisibleBumholio
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: bodhisatta]
    #25667496 - 12/09/18 09:47 AM (5 years, 1 month ago)

Can you post a link to a tek?


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"great things may come to those who wait, but only what's left by those who hustle"


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InvisiblebodhisattaMDiscordReddit
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Bumholio]
    #25667503 - 12/09/18 09:50 AM (5 years, 1 month ago)

:magicfingers:
Use magic fingers


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InvisibleBumholio
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: bodhisatta]
    #25667509 - 12/09/18 09:55 AM (5 years, 1 month ago)

:kittylaugh:


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"great things may come to those who wait, but only what's left by those who hustle"


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OfflineMH5109
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Bumholio]
    #25667530 - 12/09/18 09:59 AM (5 years, 1 month ago)

Take your scalpel and cut it, then sterilize the blade and proceed to the next procedure.


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Invisible36fuckin5
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: bodhisatta]
    #25667682 - 12/09/18 10:55 AM (5 years, 1 month ago)

Quote:

Bumholio said:
Can you post a link to a tek?




Quote:

bodhisatta said:
You can just pull it off from any spot. Or pull it all off intact as a ring.




That's the whole TEK. Just pull it off.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offline99.99
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Mycolorado]
    #25667739 - 12/09/18 11:33 AM (5 years, 1 month ago)

Quote:

Mycolorado said:
It’s pretty hard for contams to travel up under the lid and squeeze through the almost non existent space between the lid and plate and then fall on your agar.  Your technique is likely the issue.  Parafilm can split on the sharp edge of the lid after a time but shouldn’t be causing contams on your plates.  I keep plates in an old sab for an added layer of protection.  Got any pics?

This is what I see happen occasionally but I’ve never had an issue with it:



You might try plastic wrap.  People like it better than parafilm and it’s cheaper. 








If you even slightly press on the center of the agar dish it creates a vacuum or a pump that can easily transfer contaminants from the outside to the inside by pushing ore sucking the air


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InvisibleMycolorado
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: 99.99]
    #25667755 - 12/09/18 11:41 AM (5 years, 1 month ago)

That would fall under poor technique.  If you know it does that, then don’t do it. :shrug:


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InvisiblebodhisattaMDiscordReddit
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Mycolorado]
    #25667782 - 12/09/18 11:56 AM (5 years, 1 month ago)

:derfase:


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Invisible36fuckin5
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Re: Parafilm cracking on agar plate contamination risk ?? [Re: Mycolorado]
    #25667905 - 12/09/18 01:13 PM (5 years, 1 month ago)

Quote:

Mycolorado said:
That would fall under poor technique.  If you know it does that, then don’t do it. :shrug:




There's that....


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
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