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InvisibleMoxy
Registered: 06/12/13
Posts: 82
Assistance w/ Spore Print to Agar Growth?
    #25431411 - 09/02/18 09:26 PM (2 years, 8 months ago)

Hi all, first time attempting agar and have a couple plates inoculated by spore print/inoc loop on 8/25.

This is growth today 9/2, 8 days in:
 

Should I attempt to make a transfer from either plate yet?
Do these appear to be healthy?

Thanks for any help!


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InvisiblePsicomvb
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Registered: 01/13/18
Posts: 3,725
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Re: Assistance w/ Spore Print to Agar Growth? [Re: Moxy]
    #25431457 - 09/02/18 09:57 PM (2 years, 8 months ago)

They both look workable so yes do a few transfers for sure! Take transfers from the outer sections of the mycelium.


Edited by Psicomvb (09/02/18 09:59 PM)


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InvisibleMoxy
Registered: 06/12/13
Posts: 82
Re: Assistance w/ Spore Print to Agar Growth? [Re: Psicomvb]
    #25431481 - 09/02/18 10:05 PM (2 years, 8 months ago)

That's great news!

The outer edge seems much lighter than the middle - is that okay to transfer from a light section?


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InvisiblePsicomvb
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Re: Assistance w/ Spore Print to Agar Growth? [Re: Moxy]
    #25431493 - 09/02/18 10:10 PM (2 years, 8 months ago)

Yessah, but just aim for whatever spot looks like the healthiest and strongest. This early on in the agar process, though, it doesn't matter too much where you take from as long as there aren't baddies hidden in there.


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Offlinebearcreek
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Registered: 08/12/18
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Re: Assistance w/ Spore Print to Agar Growth? [Re: Psicomvb]
    #25431545 - 09/02/18 10:23 PM (2 years, 8 months ago)

The plates look fairly clean, id wait a bit for more growth, then select from there.


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InvisibleMoxy
Registered: 06/12/13
Posts: 82
Re: Assistance w/ Spore Print to Agar Growth? [Re: Psicomvb]
    #25431604 - 09/02/18 10:40 PM (2 years, 8 months ago)

Quote:

Psicomvb said:
Yessah, but just aim for whatever spot looks like the healthiest and strongest. This early on in the agar process, though, it doesn't matter too much where you take from as long as there aren't baddies hidden in there.




I think the center looks the strongest but I understand I should cut from the leading edge. I just wasn't sure if the leading edge was okay since it is so faint compared to the center.


Quote:

bearcreek said:
The plates look fairly clean, id wait a bit for more growth, then select from there.




I shouldn't be concerned with the growth hitting the side of the plate?


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Offlinehoudanny
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Registered: 07/29/18
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Re: Assistance w/ Spore Print to Agar Growth? [Re: Moxy]
    #25431616 - 09/02/18 10:43 PM (2 years, 8 months ago)

Quote:

Moxy said:

I shouldn't be concerned with the growth hitting the side of the plate?




in the next few days you'll see a spot that's looking worthy before it hits the side :thumbup:


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InvisibleMoxy
Registered: 06/12/13
Posts: 82
Re: Assistance w/ Spore Print to Agar Growth? [Re: houdanny]
    #25431728 - 09/02/18 11:18 PM (2 years, 8 months ago)

And you're for waiting? 2-1 maybe I should wait!


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InvisibleAdden
I'm a teapot
Registered: 06/04/03
Posts: 39,201
Loc: Flag
Re: Assistance w/ Spore Print to Agar Growth? [Re: Moxy]
    #25431909 - 09/03/18 01:40 AM (2 years, 8 months ago)

Quote:

Moxy said:
I shouldn't be concerned with the growth hitting the side of the plate?




By the 2nd or 3rd day there should be invisible filamentous mycelium already touching the edges, you just can't see it. We don't transfer from the edge because it's easier to miss baddies and miss your mark on good genetics. It makes it a harder fight.

Transfer immediately at signs of healthy growth.


--------------------


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InvisibleAdden
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Registered: 06/04/03
Posts: 39,201
Loc: Flag
Re: Assistance w/ Spore Print to Agar Growth? [Re: Adden]
    #25431996 - 09/03/18 02:39 AM (2 years, 8 months ago)

I can't see well on my phone (or through marker) but it looks like the area under the "3" in your second pic should be fine.

Perhaps someone with better eyes / screen resolution could chime in.


--------------------


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InvisibleMoxy
Registered: 06/12/13
Posts: 82
Re: Assistance w/ Spore Print to Agar Growth? [Re: Adden]
    #25432262 - 09/03/18 08:04 AM (2 years, 8 months ago)

Thanks for taking a look and clarifying, Adden. The area under the 3 is where I was worried about it touching the side.

I think it's my pictures rather than your screen resolution. It's tough to get a good picture with a shitty old phone.


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