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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: shroompy]
    #25639059 - 11/25/18 08:10 PM (5 years, 2 months ago)

Quote:

mr.nelsonmtman said:
Just harvested the first of my efforts. Met BE and can confirm potency as early reports indicated.


306.06g wet from 1qt spawn and 1.5qts of sub. 29.7g dry.




Nice man! How many days fruiting?

Quote:

shroompy said:
@ghiajake
I read the article on light and growth. Still can´t understand why a green LED would make any sense.. As far as I understood, its the Blue light what makes the best growth and if one would want to ad a spectrum, it would be red light, since that batch had also positive effects on the development of the FBs

or did I miss something?

besides that: nice Blobs, or Brainiacs or Enigmas or whatevers...





Well, It does kind of make sense really if you think about it. Green light isn't used by plants, and passes through down to the forest floor. Even cubes grow best under the edge of the tree canopy, where cows rest in the shade. As seen in the article, blue IS better in all aspects besides pileus formation, where green is best. Other than that, green scored second only to blue on all other aspects, and better than red, yellow, and flourescent in all aspects. I know it seems counter-intuitive since we learn "green light isn't good for anything", but it is what it is I guess...


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Offlineshroompy
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25642849 - 11/27/18 02:19 PM (5 years, 2 months ago)

Well I guess you´re right,
I will have to give it a go and get some set of green LEDs


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OfflineSolipsis
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: shroompy]
    #25643169 - 11/27/18 04:53 PM (5 years, 2 months ago)

Cool freshening up on that color spectrum info as I didn't know about the pileus formation triggering..
Gonna have to check how bad it actually is to have only blue UV-ey LED like i have.

Am definitely interested in this blob unit as like you I love the weird stuff (:

It seems though like stabilizing would involve not finding viable spores in there just one time, right? I mean it's a start though, it's one step in the process afaik.
I'm not super optimistic since it would seem like going to spores would inherently take you to a direction of the mutation reverting, but hey i am not that knowledgeable and it's that much bigger of an achievement if someone could pull of stabilizing this.

So it is confusing that someone suggests it was already pretty stabilized while at the same time it's unclear whether spores can be harvested without tissue, to ensure that you aren't just cloning it.
But please clarify if that's wrong.


Edited by Solipsis (11/27/18 04:56 PM)


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OfflineelPeligroso 5150
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Solipsis]
    #25643357 - 11/27/18 06:08 PM (5 years, 2 months ago)

Quote:

Solipsis said:
Cool freshening up on that color spectrum info as I didn't know about the pileus formation triggering..
Gonna have to check how bad it actually is to have only blue UV-ey LED like i have.

Am definitely interested in this blob unit as like you I love the weird stuff (:

It seems though like stabilizing would involve not finding viable spores in there just one time, right? I mean it's a start though, it's one step in the process afaik.
I'm not super optimistic since it would seem like going to spores would inherently take you to a direction of the mutation reverting, but hey i am not that knowledgeable and it's that much bigger of an achievement if someone could pull of stabilizing this.

So it is confusing that someone suggests it was already pretty stabilized while at the same time it's unclear whether spores can be harvested without tissue, to ensure that you aren't just cloning it.
But please clarify if that's wrong.



Most of time when cloning a fruit you are merely narrowing the gene pool in which it will pull from. This is the reason cloning mutations hardly ever repeats from clone. As there is still enough genetics left to pull from. This mutation however continues to repeat in all attempts from the cloned culture. This is why I say its semi stable. Meaning the genetic strain is almost certainly a monoculture. This one strain being this mutation that occurs to the fruiting bodies. So in theory if we can make it produce spores all the MS pool will have been eliminated already. Making it possible to recreate from spore and legal to share with the world.


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InvisibleLustyLocks
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Registered: 01/10/12
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: elPeligroso 5150]
    #25643488 - 11/27/18 07:08 PM (5 years, 2 months ago)

NICE


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InvisibleNimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: LustyLocks]
    #25644089 - 11/28/18 01:36 AM (5 years, 2 months ago)

How can i induce mutations in pe? I wanna do something like this.


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: elPeligroso 5150]
    #25644574 - 11/28/18 09:56 AM (5 years, 1 month ago)

Quote:

mr.nelsonmtman said:
Quote:

Solipsis said:
Cool freshening up on that color spectrum info as I didn't know about the pileus formation triggering..
Gonna have to check how bad it actually is to have only blue UV-ey LED like i have.

Am definitely interested in this blob unit as like you I love the weird stuff (:

It seems though like stabilizing would involve not finding viable spores in there just one time, right? I mean it's a start though, it's one step in the process afaik.
I'm not super optimistic since it would seem like going to spores would inherently take you to a direction of the mutation reverting, but hey i am not that knowledgeable and it's that much bigger of an achievement if someone could pull of stabilizing this.

So it is confusing that someone suggests it was already pretty stabilized while at the same time it's unclear whether spores can be harvested without tissue, to ensure that you aren't just cloning it.
But please clarify if that's wrong.



Most of time when cloning a fruit you are merely narrowing the gene pool in which it will pull from. This is the reason cloning mutations hardly ever repeats from clone. As there is still enough genetics left to pull from. This mutation however continues to repeat in all attempts from the cloned culture. This is why I say its semi stable. Meaning the genetic strain is almost certainly a monoculture. This one strain being this mutation that occurs to the fruiting bodies. So in theory if we can make it produce spores all the MS pool will have been eliminated already. Making it possible to recreate from spore and legal to share with the world.





This mutation was a monoculture before anyone fruited it, that's why it has "stable" growth formations. I did a monoculture test by placing three tissue samples from three different dishes on the same plate. No division line, and all myc grew together seamlessly. Granted, I started off with T3 culture, and the test was done with cuttings from my T4 transfers. If the others in their group have had different results, I don't know.

I hope someone eventually finds spores, but as "stabile" as the formations are I'm doubtful it's going to happen any time soon. It really is going to boil down to mass quantity of fruits grown by different people using different techniques and substrates to pull the spore producing genes back out. If they still exist in the isolated mutation...


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25645083 - 11/28/18 02:28 PM (5 years, 1 month ago)

So, the cliquey group now got me kicked off a FB group I adminned because they refused to be in the group with me. Some bullshit about me reporting some post in a group, yet nobody had the balls to confront me about it or even ask me if I did. Oh, and they brought up the name thing again, even though I suggested the name "Brainiac" before they ever named it and even stated in this thread that I'd call it whatever... Oh well. Some people will find any reason to exclude someone who doesn't lick their boots the way they want them to. The mutation is cool, but the time it takes to fruit is a pain in the ass. I'll finish the grows I have to finish the review, but overall so far the experience in dealing with the originators has been disappointing to say the least. I wish them luck in producing spores, and the fame they seek. I'll shift my attention to my fruiting P. subtropicalis culture.



Edited by ghiajake (11/28/18 02:40 PM)


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Offlineshroompy
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25645166 - 11/28/18 03:19 PM (5 years, 1 month ago)

I was following this Thread because it looked like something I had never seen before... But why all this drama? my fucking god! It takes the fun away of watching this project develop.

At a point I wanted somehow to be part of it! All excited and stuff.. watching the blob grow. Never thought that it will make spores, because of its biology. but still.

Now I have no interest anymore...

@ghiajake thanks for the update on lightning. looking forward to that subtropicalis log :smile:


Edited by shroompy (11/29/18 05:35 AM)


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: shroompy]
    #25645767 - 11/28/18 09:41 PM (5 years, 1 month ago)

Quote:

shroompy said:

@ghiajake thanks for the update on lighting. looking forward to that subtropicalis log :smile:




No problem, it was great info so it needed to be shared. :wink: I may do a Penis Envy grow log in between. I'll link it in my sig in place of this thread once I get it going.


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OfflineFishLevelMidnight
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25645918 - 11/29/18 12:28 AM (5 years, 1 month ago)

I don’t think it will ever produce spores.

What should be done is mix the myc from this and from a PE clone and see if you can keep the odd blobbing while maintaining some normal ish fruits, or maybe blobs and brains first flush and more normal fruits second flush?

If this is a monoculture you will never get spores. The chances that the genetics are broken is high, and thus unlikely that it will spontaneously repair the ability to form spore producing structures


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: FishLevelMidnight]
    #25645928 - 11/29/18 12:45 AM (5 years, 1 month ago)

Quote:

fishermansjc said:
I don’t think it will ever produce spores.

What should be done is mix the myc from this and from a PE clone and see if you can keep the odd blobbing while maintaining some normal ish fruits, or maybe blobs and brains first flush and more normal fruits second flush?

If this is a monoculture you will never get spores. The chances that the genetics are broken is high, and thus unlikely that it will spontaneously repair the ability to form spore producing structures





I agree, and is why I suggested stressing the culture through abnormal conditions to try and pull out some latent genetics.


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25645929 - 11/29/18 12:47 AM (5 years, 1 month ago)

I have started my new PE grow log here. You can also find it in my signature.


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OfflineSolipsis
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25647034 - 11/29/18 04:58 PM (5 years, 1 month ago)

Respect for that explanation nelson and good luck jake - I think I am with fisherman on the expectations, it was what i was trying to say earlier.


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Solipsis]
    #25656234 - 12/04/18 07:17 AM (5 years, 1 month ago)

Updated OP with newest pics.


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InvisibleNimph
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25656884 - 12/04/18 01:24 PM (5 years, 1 month ago)

While i dont think this culture by itself will ever sporeulate by itself a similar mutant might.

Have you thought of introducing the genes to spore into its dna? That seems like the way to go.


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Nimph]
    #25669005 - 12/09/18 10:37 PM (5 years, 1 month ago)

That would be up to the people that are continuing to work with the culture. :smile:


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InvisibleNimph
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25669074 - 12/09/18 11:47 PM (5 years, 1 month ago)

I hate to the debby downer but i dont think the same culture will just throw out spores one day.


Im not hating on the project i want yall to get it cause ya know whole new sp!!!!!!!!!!!!!!!!!!!! Thats some next level science shit.
With this im just spit balling ideas this is beyond my experience by a long shot.


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Nimph]
    #25669081 - 12/09/18 11:50 PM (5 years, 1 month ago)

Quote:

Nimph said:
With this im just spit balling ideas this is beyond my experience by a long shot.




:thumbup:


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Offlineghiajake
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Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: ghiajake]
    #25671352 - 12/11/18 01:41 AM (5 years, 1 month ago)

Updated OP a couple days ago with the final pics. Had a Mycogone infestation and had to end the grow before it contaminated my next experiment.


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