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Offlineghiajake
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Ghia's Tidalwave Blob Thread (aka Brainiac) (aka Enigma) * 10
    #25427363 - 08/31/18 09:57 PM (5 years, 4 months ago)

Ghia's "Tidalwave" Mutant Blob Thread (aka "Brainiac" substrain stabilization experiment)



    Those of you who know me well know I have a love for the truly exotic. Well, I have a new project to work on... These incredibly gnarly mutant blob genetics were donated (for scientific purposes) by SWIM (not someone from Shroomery, so please be kind admins).
 
    Some back story of the Tidalwave strain for those who aren't aware, this strain is "B+" and "Penis Envy" genetics crossed. There are others working on the stabilization of the Tidalwave strain that have genetics ranging from normal cube taxonomy to fat stiped PE-like fruits with a slightly larger cap, that produce more spores than PE.

    SWIM however got this funky brain-like mutation, that apparently is much more potent than a normal cube. And maybe more than PE... An interesting difference (he says) is that the effects are more like Ecstasy (with visuals) than normal cube trips. SWIM has shared photos from repeating flushes that continued to produce these blobs. There are a few other people working on this as well, none of which are from here. SWIM is currently working on the backstory for me to share here. I will edit the OP when he provides the text and pics for me.

    My interest in this substrain is mostly the fruit formations, which seem to form like the folds in a brain. It has as of yet not produced spores, which is another goal of mine. I will also be experimenting with a few different fruiting methods and substrates to see if that effects fruit formation. The end goal is to produce a stable mutant that can produce a small amount of spores and be fruited like a "Lion's Mane" or "Hen of the Woods".

So, without further adieu... Let me introduce the star of this show..... Brainiac!



     




    Moving forward, here are my first steps I will be taking. Pasty's "No-pour" tek is a great method, but I prefer the glass half-pint Kerr brand jars and a simple modified lid. I'll start of by describing my agar method. I wrote my agar recipe on the lid of the agar jar  so that I quit forgetting it. :wink: This has been scaled so that it can be used for a large amount of dishes, or just a few. The recipe is:

1 part grain-soak water to 9 parts clean water.
1 heaping 1/8 tsp of agar powder per jar (2g per 100ml of broth).

    Easy! I like that the myc starts off on the same nutrient profile as the grains it'll be transferred to later, plus you don't have to mess with any other nutrients bases (nasty malt extract, etc)

    I keep my GS water in the freezer and just thaw some off in the microwave as needed. For this small application, I used a syringe to squirt 2.5ml of raw GS water into each jar. I then added the agar powder and hot clean water. For larger scale I usually mix the hot and GS waters in a jar, then use a large syringe to measure 25ml of "broth" into each jar after putting the powder in. Use a spoon to stir the contents, then PC for 30 minutes at 15 PSI. I don't often get to use my small electric PC, but when I do it's awesome!







    While I've got the lil' PC out, I'm doing a few half-pint grain jars as well. Two will be used for G2G, and two I will case and attempt to fruit directly from the jars. This batch of grains is red milo with a dash of coffee in the grain soak water, PC'd at 15 PSI for 60 minutes. Next batch of grains I will be returning to my preferred mix of red milo/fescue grass seed at 1:1 ratio by volume (not weight).



    I will be updating in the OP as I go, so make sure you re-check it to see progress. To be clear, this is a blob mutation, NOT a sclerotia. Cubensis do not produce sclerotia... :wink:

UPDATE: 9/14/18

Colonizing wonderfully, just look at those rhizos!!!




UPDATE: 9/17/18

It's finally transfer day! Took some T5 samples from the ropey rhizos in the first pic, then noc'd the 3 jars of my T4 culture to grain (1:1 milo/grass seed. One dish is a T5 transfer with samples from three T4 dishes, to see if the cloned tissue is a monoculture, or if the consecutive transfers has allowed genetic variances. The new round of agar was mixed with half my new normal nutrient level (1:25 instead of 1:12.5, hoping that the lower nutrient makes the myc go even more rhizo.





UPDATE: 10/14/18

This myc is just incredible! Contrary to usual, the myc gets more rhizo the more nutrients you give it.




Here are the three cased half-pint jars of straight grain. They started pinning about 1-1.5 weeks after I cased it.








I've got six 5 pounds bags of verm/perlite substrate colonizing now as well.




UPDATE: 10/21/18





UPDATE: 10/26/18





UPDATE: 11/1/18




UPDATE: 11/4/18

So, after 25 days of fruiting I decided to call the "Brainiac" test jars done. I used a sprayer to remove the casing material, and broke up the fruits to clean them better. Got just under 70g wet weight. I'm also dehydrating the colonized milo substrate to test the myc for micro-dosing viability.

What did I learn most from the test jars? Don't use a casing with the "Brainiac", and use a large enough substrate to produce larger blobs. Otherwise the time it takes to fruit them isn't worth the harvest.






Next for the Brainiac will be fruiting 5lb verm/perlite blocks in filter patch bags. Updates will follow in the OP again when I have more to update.


UPDATE: 11/11/18


And we continue.... I ended up having to get a 4'x4'x6.5' Mylar grow tent to house my new automated martha and storage shelving to keep proper colonizing and fruiting temps. I added a string of green LED's to the martha because of this great article on the effects of monochromatic LED lighting. I highly recommend reading it. In fact, a couple days after adding it is when I noticed all the "Brainiac" blocks where covered in hyphal knots and pins.

The 9 blocks on the top three shelves in the martha are the "Brainiac" blocks. Sorry about the striping in the photos, it's the effect of the blue and green lighting.





UPDATE: 11/22/18


Here's some fin pin porn! :wink: Zoom in, my camera is a decent one.




UPDATE: 12/4/18

These things are SOOOOO slow. Getting a couple good formations though. I do seem to be getting "fuzzy feet" on the blocks, which I'm assuming is from too high RH. Moved the blocks to the bottom two shelves of the Martha since they seem to run a bit lower than the top two. I'll give them about 3.5 weeks left as that's when the next batch of PE will be going into the Martha and I'll need the space. Here's pics.




UPDATE: 12/10/18

I had to pull the blocks as the fuzzy blobs showed themselves to be Mycogone (Wet Bubble). In reading more on the contamination I found that Mygone usually comes from too low moisture content (below 60%), and too low pasteurization temps. Mycogone dies at 140F (60C), so proper pasteurization temps should always kill it. Oh well, it was a fun grow for sure, and I got around 311 g wet weight. Will be drying the fruits to save for when I am in the mood to test potency. I very rarely partake anymore, just like growing weird things.... Have a couple friends who have been waiting patiently to guinea-pig for me though. :smile:

Here are pics of the two largest clusters w/ weights, and the last pic is all the rest of the small clusters.




My final assessment of the initial test grow is that as aggressive as the culture is it seems to require a bit higher level of nutrient than average. Since this substrate was straight verm/perlite, the only nutrient provided was the quart of grain spawn. I'd say any normal substrate recipe would be sufficient, and would probably love Hpoo (pretty sure others have tested Hpoo already). I still think the length of time it takes for fruit formation is a problem, and could result in higher chances of contamination after first flush. Another mildly annoying point is that with the lack of a cap, it is really hard to tell when the fruit is actually ready to pick or if it'll just keep growing. I hope to see someone test just how long a single cluster will continue to grow before it stalls or shows some sign of definitive "ripeness".

I have a test dish left that has begun pinning on the agar, so I'll probably keep working with it in the background for a while. It'll probably be a few months before I re-visit fruiting this weirdo, but when I do I'll post any results here.


Edited by ghiajake (09/21/22 08:33 AM)


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: ghiajake]
    #25427393 - 08/31/18 10:19 PM (5 years, 4 months ago)

One of my first predictions is that this formation should be able to tolerate lower RH than normal, but will draw much more water from the substrate each flush.


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: Pastywhyte] * 1
    #25428219 - 09/01/18 10:52 AM (5 years, 4 months ago)

Quote:

Pastywhyte said:
That's a hell of a blob jake. What that sucker weigh?




Gotta double check with SWIM, but I'm pretty sure the wet-weight was over 900g.


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: bodhisatta]
    #25428257 - 09/01/18 11:13 AM (5 years, 4 months ago)

Quote:

bodhisatta said:
Reminds me a bit of this
https://www.shroomery.org/forums/showflat.php/Number/25285598

I wonder if its a pinning initiation error. Pins are just specialized mycelium. These blobs look like extremely dense formations of mycelium that are not pins.

When i examined my blob thing from the above thread it was really just a bunch of strands of rhizomorphic mycelium jam packed together.





Hey Bohdi, how ya been man? Yeah, I've had this happen with P. ostreatus too. Some of SWIM's blobs did look more like your blobs, but I haven't ever seen this brain-like folding formations. It's almost like a Sparassis crispa (cauliflower mushroom).


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Re: Tidalwave Blob Thread [Re: ghiajake] * 1
    #25428284 - 09/01/18 11:28 AM (5 years, 4 months ago)

Just asked him. Wet-weight was 932g.


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: Pastywhyte]
    #25428408 - 09/01/18 12:42 PM (5 years, 4 months ago)

Quote:

mr.nelsonmtman said:
should i be adding to this log? with steps and updates? or are you just documenting your work with these cultures? Im running 3 different cultures of the tidalwave variant side by side as a comparative. One from spore, one from an isolated culture and the two cultures from the "blobs".




Feel free to post updates of your blobs here. If you have other non-blob Tidalwaves, I'd suggest starting your own threads for those. I do want to link all the various Tidalwave threads together though, so if you do start your own threads please make a link here for them.

This goes for everyone working on the Tidalwave strain.

Quote:

Pastywhyte said:

Wow. Bizarre. Probably the biggest blob on record, I think Uncle Rico had the previous record holder at 400+




Are there other blob threads that they've gotten these brain-like folds, or are they more the globular formations?


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: space_shrooms] * 1
    #25429736 - 09/02/18 12:19 AM (5 years, 4 months ago)

Got the 4 agar and 4 tiny grain jars noc'd tonight, it has officially began! Now we wait... :spirit:


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Re: Tidalwave Blob Thread [Re: PrimalSoup]
    #25429758 - 09/02/18 12:49 AM (5 years, 4 months ago)

Hi Primal, good to have ya join the party. :smile: I saw your last thread. Guess you took a break too huh? I gave up growing anything for a couple years, even spent a year up in MI managing my ex's family's dispensary. Had 4 patients I was caregiver to as well.


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Re: Tidalwave Blob Thread [Re: PrimalSoup]
    #25429783 - 09/02/18 01:14 AM (5 years, 4 months ago)

Quote:

PrimalSoup said:
I've got some interesting projects I'd like to work on if I can just get my goddamn sterile culture tek actually working again. :mushroom2:




You still haven't built a flowhood? Check out the "Sterile workspace" link in my sig. I love the damn thing, don't even need to wear a mask! :smile:


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: mudbutt]
    #25468560 - 09/17/18 10:44 PM (5 years, 4 months ago)

Updated the OP.


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Offlineghiajake
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Re: Tidalwave Blob Thread [Re: mushboy]
    #25534354 - 10/13/18 11:45 AM (5 years, 3 months ago)

I'll update the OP after I get off work tonight, but I have fin pins... Bodhi, they actually do form pins, with no caps.

It's amazing how some people get butthurt when you don't bow down and praise them for accidentally creating a blob mutation that they had no idea would mutate.... I was kicked out of the Tidalwave Blob Fakebook group because.... Well, I'll just post the ignorant shit...


" Yeah, I've been tore between him too. Personal feelings aside, it's just the look at me and what I'm doing. No mention of a group effort with him being a part but more taking the lead with it. He's dropped big names repeatedly to make it known he's somebody important and raves about all his views. It's the barggart part of him that I personally dislike. That aside, I have seen him sharing cultures and stuff among the group. I can tolerate him just fine and as I've said, it's your show. If you want him ill put him back in. He actually has been working on the blob and posting to the group. The other guys were a definite threat to our agenda. I have no doubt they were looking to sell the cultures. I didn't know that guy was the Dom. I've seen his postes but didn't make the connection. I just more than anything want the credit of this thing to go to you. Why I put your name on every dish I make of it. I'll put Jacob back if that's what you want. I don't feel he's a danger, just one to brag about it and leading others to believe he's somehow heading the thing when it's a group effort thanks to you. If he sees everythibg that's been said today, he should get the idea real quick. Just give me the word brother, your in the captains chair."

If you're going to insult someone, maybe you should make sure you've used proper punctuation and spelling... :ohyou:

Oh well, fuck it. Like I said on FB, I'm not going to credit Alan R everytime I post about my allenii bed, and now I'm going to do my best to NEVER give credit to the person who accidentally created this. Dude sectored out a bunch of dishes, then sent them to another guy who actually fruited it and discovered the mutation. The second guy is who I'll give credit to, and that's it. I'll take credit for MY grows every time, because I'm the one growing my grows.... There is no "group effort", there's a bunch of guys who have separate grows using different grains, subs, and fruiting teks, all trying to be the one who finds a way to stabilize it. It's not like they are a team of people working in a fucking lab... This butthurt fuck ain't done shit for the world of amateur mycology other than give a few active prints/cultures away to people and sector out this myc without ever fruiting it to test the isolation's viability. By his stupid-ass mentality, a resume with personal references means you're a braggart. Oh, wait... I mean "barggart"! :laugh2:


Edited by ghiajake (10/13/18 12:29 PM)


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Re: Tidalwave Blob Thread [Re: FishLevelMidnight]
    #25535358 - 10/13/18 08:29 PM (5 years, 3 months ago)

Quote:

fishermansjc said:
I got a box knotting up as we speak. Is taking longer for pin development.




I've noticed the pins take much longer to grow than normal cube pins.


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Re: Tidalwave Blob Thread [Re: ghiajake]
    #25535618 - 10/13/18 10:27 PM (5 years, 3 months ago)

OP updated.


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Re: Tidalwave Blob Thread [Re: PrimalSoup]
    #25586795 - 11/02/18 12:47 PM (5 years, 2 months ago)

Updated the OP.


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Re: Tidalwave Blob Thread [Re: FishLevelMidnight]
    #25586813 - 11/02/18 12:57 PM (5 years, 2 months ago)

Quote:

fishermansjc said:
Cool that the mutation seems to have carried with the cloning. Looking forward to see how they turn out!





Yeah, everyone that got the culture seems to be having the same formation as the original blob. Mine is acting weird, which could be because mine has a casing and is on straight grain.


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Re: Tidalwave Blob Thread [Re: elasticaltiger]
    #25587685 - 11/02/18 07:28 PM (5 years, 2 months ago)

Quote:

elasticaltiger said:
Damn. Braniac be all over the place. Super cool how it bruised all the way through.

Haven't read the whole thread. How was the trip?





Haven't tried it yet, but swim says it's like rolling on X, with visuals. I imagine the potency is pretty high, judging by the bluing of the blobs and the myc itself.


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Re: Tidalwave Blob Thread [Re: verum subsequentis]
    #25587690 - 11/02/18 07:29 PM (5 years, 2 months ago)

Quote:

verum subsequentis said:
well i'll be a son of a diddly. i've seen some strangeness in my growing days but that is quite weird indeed. i wonder how the potency will be and if you'll be able to find spores.




Hoping for spores, but we'll see. They don't seem to produce a cap at all.


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Re: Tidalwave Blob Thread [Re: ghiajake]
    #25590324 - 11/03/18 10:25 PM (5 years, 2 months ago)

Updated the OP with the finale of the 3 straight grain test jars.


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Re: Tidalwave Blob Thread [Re: verum subsequentis]
    #25590449 - 11/03/18 11:38 PM (5 years, 2 months ago)

Quote:

verum subsequentis said:
that shit is crazy! find any spores?




Nope.


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Re: Tidalwave Blob Thread [Re: R.I.P.Zappa]
    #25612193 - 11/13/18 02:05 PM (5 years, 2 months ago)

Birthed the 5lb blocks last night. Updated in OP.


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