Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,846
Loc: Indiana
Last seen: 14 hours, 5 minutes
Ghia's Tidalwave Blob Thread (aka Brainiac) (aka Enigma) * 10
    #25427363 - 08/31/18 09:57 PM (5 years, 4 months ago)

Ghia's "Tidalwave" Mutant Blob Thread (aka "Brainiac" substrain stabilization experiment)



    Those of you who know me well know I have a love for the truly exotic. Well, I have a new project to work on... These incredibly gnarly mutant blob genetics were donated (for scientific purposes) by SWIM (not someone from Shroomery, so please be kind admins).
 
    Some back story of the Tidalwave strain for those who aren't aware, this strain is "B+" and "Penis Envy" genetics crossed. There are others working on the stabilization of the Tidalwave strain that have genetics ranging from normal cube taxonomy to fat stiped PE-like fruits with a slightly larger cap, that produce more spores than PE.

    SWIM however got this funky brain-like mutation, that apparently is much more potent than a normal cube. And maybe more than PE... An interesting difference (he says) is that the effects are more like Ecstasy (with visuals) than normal cube trips. SWIM has shared photos from repeating flushes that continued to produce these blobs. There are a few other people working on this as well, none of which are from here. SWIM is currently working on the backstory for me to share here. I will edit the OP when he provides the text and pics for me.

    My interest in this substrain is mostly the fruit formations, which seem to form like the folds in a brain. It has as of yet not produced spores, which is another goal of mine. I will also be experimenting with a few different fruiting methods and substrates to see if that effects fruit formation. The end goal is to produce a stable mutant that can produce a small amount of spores and be fruited like a "Lion's Mane" or "Hen of the Woods".

So, without further adieu... Let me introduce the star of this show..... Brainiac!



     




    Moving forward, here are my first steps I will be taking. Pasty's "No-pour" tek is a great method, but I prefer the glass half-pint Kerr brand jars and a simple modified lid. I'll start of by describing my agar method. I wrote my agar recipe on the lid of the agar jar  so that I quit forgetting it. :wink: This has been scaled so that it can be used for a large amount of dishes, or just a few. The recipe is:

1 part grain-soak water to 9 parts clean water.
1 heaping 1/8 tsp of agar powder per jar (2g per 100ml of broth).

    Easy! I like that the myc starts off on the same nutrient profile as the grains it'll be transferred to later, plus you don't have to mess with any other nutrients bases (nasty malt extract, etc)

    I keep my GS water in the freezer and just thaw some off in the microwave as needed. For this small application, I used a syringe to squirt 2.5ml of raw GS water into each jar. I then added the agar powder and hot clean water. For larger scale I usually mix the hot and GS waters in a jar, then use a large syringe to measure 25ml of "broth" into each jar after putting the powder in. Use a spoon to stir the contents, then PC for 30 minutes at 15 PSI. I don't often get to use my small electric PC, but when I do it's awesome!







    While I've got the lil' PC out, I'm doing a few half-pint grain jars as well. Two will be used for G2G, and two I will case and attempt to fruit directly from the jars. This batch of grains is red milo with a dash of coffee in the grain soak water, PC'd at 15 PSI for 60 minutes. Next batch of grains I will be returning to my preferred mix of red milo/fescue grass seed at 1:1 ratio by volume (not weight).



    I will be updating in the OP as I go, so make sure you re-check it to see progress. To be clear, this is a blob mutation, NOT a sclerotia. Cubensis do not produce sclerotia... :wink:

UPDATE: 9/14/18

Colonizing wonderfully, just look at those rhizos!!!




UPDATE: 9/17/18

It's finally transfer day! Took some T5 samples from the ropey rhizos in the first pic, then noc'd the 3 jars of my T4 culture to grain (1:1 milo/grass seed. One dish is a T5 transfer with samples from three T4 dishes, to see if the cloned tissue is a monoculture, or if the consecutive transfers has allowed genetic variances. The new round of agar was mixed with half my new normal nutrient level (1:25 instead of 1:12.5, hoping that the lower nutrient makes the myc go even more rhizo.





UPDATE: 10/14/18

This myc is just incredible! Contrary to usual, the myc gets more rhizo the more nutrients you give it.




Here are the three cased half-pint jars of straight grain. They started pinning about 1-1.5 weeks after I cased it.








I've got six 5 pounds bags of verm/perlite substrate colonizing now as well.




UPDATE: 10/21/18





UPDATE: 10/26/18





UPDATE: 11/1/18




UPDATE: 11/4/18

So, after 25 days of fruiting I decided to call the "Brainiac" test jars done. I used a sprayer to remove the casing material, and broke up the fruits to clean them better. Got just under 70g wet weight. I'm also dehydrating the colonized milo substrate to test the myc for micro-dosing viability.

What did I learn most from the test jars? Don't use a casing with the "Brainiac", and use a large enough substrate to produce larger blobs. Otherwise the time it takes to fruit them isn't worth the harvest.






Next for the Brainiac will be fruiting 5lb verm/perlite blocks in filter patch bags. Updates will follow in the OP again when I have more to update.


UPDATE: 11/11/18


And we continue.... I ended up having to get a 4'x4'x6.5' Mylar grow tent to house my new automated martha and storage shelving to keep proper colonizing and fruiting temps. I added a string of green LED's to the martha because of this great article on the effects of monochromatic LED lighting. I highly recommend reading it. In fact, a couple days after adding it is when I noticed all the "Brainiac" blocks where covered in hyphal knots and pins.

The 9 blocks on the top three shelves in the martha are the "Brainiac" blocks. Sorry about the striping in the photos, it's the effect of the blue and green lighting.





UPDATE: 11/22/18


Here's some fin pin porn! :wink: Zoom in, my camera is a decent one.




UPDATE: 12/4/18

These things are SOOOOO slow. Getting a couple good formations though. I do seem to be getting "fuzzy feet" on the blocks, which I'm assuming is from too high RH. Moved the blocks to the bottom two shelves of the Martha since they seem to run a bit lower than the top two. I'll give them about 3.5 weeks left as that's when the next batch of PE will be going into the Martha and I'll need the space. Here's pics.




UPDATE: 12/10/18

I had to pull the blocks as the fuzzy blobs showed themselves to be Mycogone (Wet Bubble). In reading more on the contamination I found that Mygone usually comes from too low moisture content (below 60%), and too low pasteurization temps. Mycogone dies at 140F (60C), so proper pasteurization temps should always kill it. Oh well, it was a fun grow for sure, and I got around 311 g wet weight. Will be drying the fruits to save for when I am in the mood to test potency. I very rarely partake anymore, just like growing weird things.... Have a couple friends who have been waiting patiently to guinea-pig for me though. :smile:

Here are pics of the two largest clusters w/ weights, and the last pic is all the rest of the small clusters.




My final assessment of the initial test grow is that as aggressive as the culture is it seems to require a bit higher level of nutrient than average. Since this substrate was straight verm/perlite, the only nutrient provided was the quart of grain spawn. I'd say any normal substrate recipe would be sufficient, and would probably love Hpoo (pretty sure others have tested Hpoo already). I still think the length of time it takes for fruit formation is a problem, and could result in higher chances of contamination after first flush. Another mildly annoying point is that with the lack of a cap, it is really hard to tell when the fruit is actually ready to pick or if it'll just keep growing. I hope to see someone test just how long a single cluster will continue to grow before it stalls or shows some sign of definitive "ripeness".

I have a test dish left that has begun pinning on the agar, so I'll probably keep working with it in the background for a while. It'll probably be a few months before I re-visit fruiting this weirdo, but when I do I'll post any results here.


Edited by ghiajake (09/21/22 08:33 AM)


Extras: Unfilter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: One of Us] * 3
    #27084060 - 12/11/20 02:12 PM (3 years, 1 month ago)

One month of fruiting. 4 quart spawn bag. 150 grams dry.



Edited by Munchauzen (12/11/20 02:40 PM)


Extras: Unfilter Print Post Top
OfflineelPeligroso 5150
Stranger
Male User Gallery


Registered: 11/13/17
Posts: 166
Last seen: 2 years, 1 month
Re: Tidalwave Blob Thread [Re: Germs] * 2
    #25620056 - 11/17/18 02:14 AM (5 years, 2 months ago)

Appreciate the feedback. thank you. Sorry if my understanding of the word SWIM was misunderstood in this forum as it is the only way I've ever heard it used and refers to the individual using it. That is dated though and I understand. As far as the name goes. Of course OP can call his work what ever he likes as it is his work. The original work that produced the mutation was not my own. So out of respect for that individual I will refer to our work as it is now been named. No offense to OP or this forum or you what so ever. More respect for the individual work of the individual responsible is all. Would also love to get this to some one to put under the scope as well, as the mycelium displays two very different characteristics while colonizing simultaneously. I will upload the pic. I'm new to the site and hope I'm not out of bounds or out of place and hope to become a contributing member of the community. Mush Luv!


Edited by elPeligroso 5150 (11/17/18 02:20 AM)


Extras: Unfilter Print Post Top
OfflineCHOOS
Stranger
 User Gallery
Registered: 08/05/15
Posts: 105
Last seen: 3 years, 8 months
Re: Tidalwave Blob Thread [Re: elPeligroso 5150] * 2
    #25620231 - 11/17/18 06:12 AM (5 years, 2 months ago)

Quote:

mr.nelsonmtman said:in hopes of identifying gill tissues to swab for spores.




Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please! Please!


Extras: Unfilter Print Post Top
OfflinePrimalSoup
hyperspatial illuminations
Other User Gallery


Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 1 year, 5 months
Trusted Cultivator
Re: Tidalwave Blob Thread [Re: ghiajake] * 2
    #25624375 - 11/18/18 11:54 PM (5 years, 2 months ago)

Keep on keepin' on, the strain doesn't know where the fuck it came from.  :goodday:


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


Extras: Unfilter Print Post Top
Invisiblemustangbob3
Mad Myrmecologist
I'm a teapot


Registered: 10/15/14
Posts: 1,685
Re: Tidalwave Blob Thread [Re: bodhisatta] * 1
    #25428204 - 09/01/18 10:44 AM (5 years, 4 months ago)

Quote:

bodhisatta said:
Reminds me a bit of this
https://www.shroomery.org/forums/showflat.php/Number/25285598

I wonder if its a pinning initiation error. Pins are just specialized mycelium. These blobs look like extremely dense formations of mycelium that are not pins.

When i examined my blob thing from the above thread it was really just a bunch of strands of rhizomorphic mycelium jam packed together.




wut? lol all fruits or blobs are just strands of rhizomorphic mycelium jam packed together!

thats what mushrooms are.


--------------------



Extras: Unfilter Print Post Top
Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,846
Loc: Indiana
Last seen: 14 hours, 5 minutes
Re: Tidalwave Blob Thread [Re: Pastywhyte] * 1
    #25428219 - 09/01/18 10:52 AM (5 years, 4 months ago)

Quote:

Pastywhyte said:
That's a hell of a blob jake. What that sucker weigh?




Gotta double check with SWIM, but I'm pretty sure the wet-weight was over 900g.


Extras: Unfilter Print Post Top
Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,846
Loc: Indiana
Last seen: 14 hours, 5 minutes
Re: Tidalwave Blob Thread [Re: ghiajake] * 1
    #25428284 - 09/01/18 11:28 AM (5 years, 4 months ago)

Just asked him. Wet-weight was 932g.


Extras: Unfilter Print Post Top
Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,846
Loc: Indiana
Last seen: 14 hours, 5 minutes
Re: Tidalwave Blob Thread [Re: space_shrooms] * 1
    #25429736 - 09/02/18 12:19 AM (5 years, 4 months ago)

Got the 4 agar and 4 tiny grain jars noc'd tonight, it has officially began! Now we wait... :spirit:


Extras: Unfilter Print Post Top
OfflinePrimalSoup
hyperspatial illuminations
Other User Gallery


Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 1 year, 5 months
Trusted Cultivator
Re: Tidalwave Blob Thread [Re: ghiajake] * 1
    #25429752 - 09/02/18 12:40 AM (5 years, 4 months ago)

Cool thread!  I'm in.  :popcorn:


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


Extras: Unfilter Print Post Top
OfflinePrimalSoup
hyperspatial illuminations
Other User Gallery


Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 1 year, 5 months
Trusted Cultivator
Re: Tidalwave Blob Thread [Re: Germs] * 1
    #25534546 - 10/13/18 01:17 PM (5 years, 3 months ago)

Pins on fins see we shall. :yoda2:


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 


Extras: Unfilter Print Post Top
OfflineelPeligroso 5150
Stranger
Male User Gallery


Registered: 11/13/17
Posts: 166
Last seen: 2 years, 1 month
Re: Tidalwave Blob Thread [Re: Dabadoodledoo710] * 1
    #25637392 - 11/25/18 01:24 AM (5 years, 2 months ago)

Just harvested the first of my efforts. Met BE and can confirm potency as early reports indicated.


306.06g wet from 1qt spawn and 1.5qts of sub. 29.7g dry.


Edited by elPeligroso 5150 (11/25/18 12:17 PM)


Extras: Unfilter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,808
Loc: Canada Flag
Trusted Cultivator
Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: A.k.a] * 1
    #26812321 - 07/09/20 08:09 AM (3 years, 6 months ago)

Enigma isn’t a variety in the sense that it’s sterile and all cultures of it you see are the same clone floating around. It came from the variety “tidal wave” hence the name of this thread.


Extras: Unfilter Print Post Top
OfflinePupMarvel
MushroomDog
Male


Registered: 02/17/19
Posts: 38
Loc: BC Canada
Last seen: 1 month, 22 days
Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Pastywhyte] * 1
    #26836597 - 07/21/20 05:08 PM (3 years, 6 months ago)

I've been super thrilled with my first attempt with Enigma, she seems to be going well. It's been great watching her grow she really goes through a number of looks. This tub was spawned on June 3rd.





Extras: Unfilter Print Post Top
OfflineelPeligroso 5150
Stranger
Male User Gallery


Registered: 11/13/17
Posts: 166
Last seen: 2 years, 1 month
Re: Ghia's Tidalwave Blob Thread (aka Brainiac) [Re: Munchauzen] * 1
    #27087617 - 12/13/20 05:14 PM (3 years, 1 month ago)

Glad too see she is making her rounds and finding capable hands! Good looking fruits!


Extras: Unfilter Print Post Top
Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,846
Loc: Indiana
Last seen: 14 hours, 5 minutes
Re: Tidalwave Blob Thread [Re: ghiajake]
    #25427393 - 08/31/18 10:19 PM (5 years, 4 months ago)

One of my first predictions is that this formation should be able to tolerate lower RH than normal, but will draw much more water from the substrate each flush.


Extras: Unfilter Print Post Top
InvisibleSubnet Mask
 User Gallery

Registered: 05/08/15
Posts: 903
Loc: Flag
Re: Tidalwave Blob Thread [Re: ghiajake]
    #25427488 - 08/31/18 11:54 PM (5 years, 4 months ago)

:popcorn:


Extras: Unfilter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Tidalwave Blob Thread [Re: Subnet Mask]
    #25427534 - 09/01/18 12:24 AM (5 years, 4 months ago)

This is crazy man. What a trip.


Extras: Unfilter Print Post Top
Offlineentheo_heathen
Unrepentant Heathen
I'm a teapot


Registered: 01/24/08
Posts: 401
Last seen: 2 years, 11 days
Re: Tidalwave Blob Thread [Re: ghiajake]
    #25427589 - 09/01/18 12:57 AM (5 years, 4 months ago)

:electrickoolaid:

I Fucking Love This Mutant!

SO STOKED ON THIS THREAD
:trippinbawelz:


--------------------
Entheogenic Heathen

I Am Shroomery (And So Can You!)  ~
RogerRabbit    bodhisatta    FrankHorrigan
TranscendingLife    Violet    c10h12n2o

Trade List

@azurefungi


Extras: Unfilter Print Post Top
Offlinescifipirate
Jug Fuckler
Male User Gallery


Registered: 01/17/09
Posts: 870
Loc: FL Flag
Last seen: 1 year, 11 months
Re: Tidalwave Blob Thread [Re: entheo_heathen]
    #25427649 - 09/01/18 01:40 AM (5 years, 4 months ago)

Cool


Extras: Unfilter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Next >  [ show all ]

Shop: North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Post your "TidalWave"/"Brainiac"/"Enigma" Photos here
( 1 2 3 4 ... 18 19 )
Mr. Funguy 33,359 371 11/26/22 12:50 PM
by doubleD
* Liquid Culture De-Mystified: Ghia Style
( 1 2 3 4 5 6 all )
ghiajake 23,387 115 10/05/14 03:59 PM
by eatyualive
* Ghia's Easy Outdoor Mushroom Beds
( 1 2 3 4 5 6 7 8 all )
ghiajake 20,576 141 12/14/23 09:17 PM
by LewDoja
* Ghia's Lime-Baked Bulk Pasteurizing Method ghiajake 2,099 14 02/07/22 02:31 PM
by ghiajake
* Bleach versus the Blob Hippie3 2,156 3 09/01/03 07:42 AM
by MAIA
* Shroom BLOBS!!!! KegTheRipper 5,130 2 12/13/02 03:34 PM
by Llamanose
* the blob? icemen 3,311 13 12/18/03 10:33 PM
by icemen
* The Blob! 2shrewmen 2,705 3 05/13/02 08:36 PM
by 2shrewmen

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
78,462 topic views. 17 members, 157 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Date | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.009 seconds on 16 queries.