Home | Community | Message Board


MRCA Tyroler Gluckspilze
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Kratom Powder For Sale   Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, Agar, Petri Dish, Potato Dextrose

Jump to first unread post. Pages: 1
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Agar second transfer ?
    #25426605 - 08/31/18 06:28 PM (2 years, 8 months ago)

This is my second transfer from multispore syringe how big should I let it grow before inoculating, jar? If I read right there’s no need for third transfer if second is clean, I think it appears clean but don’t know how big to let it get before cutting a chunk out?  I uploaded 2 pictures the second dish is it a contam growing on left side?  or is this just a different type of myc growth..  thnaks



Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineNitrogoxygen
Timelord

Registered: 02/19/18
Posts: 533
Loc: A.N.U.S.
Last seen: 2 years, 11 days
Re: Agar second transfer ? [Re: bakenast]
    #25426657 - 08/31/18 06:50 PM (2 years, 8 months ago)

Quote:

bakenast said:
This is my second transfer from multispore syringe how big should I let it grow before inoculating, jar? If I read right there’s no need for third transfer if second is clean, I think it appears clean but don’t know how big to let it get before cutting a chunk out?  I uploaded 2 pictures the second dish is it a contam growing on left side?  or is this just a different type of myc growth..  thnaks





There is a name for the growth at 7 o’clock to 11 o’clock on that pic on the right, it’s suspicious. You want all spikes! Left looks good, no reason. TO NOT make more plates for later right?!?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Nitrogoxygen]
    #25426786 - 08/31/18 07:40 PM (2 years, 8 months ago)

Well I have others similar to the left,, the pic on the right I just put there for feedback, since it looked funny , my other plates can grow out and save, I guess my question is how big of a wedge is normally used for a quart grain jar?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineNataraja_Shiva
Philosophus


Registered: 05/15/18
Posts: 325
Loc: Earth
Last seen: 3 months, 19 days
Re: Agar second transfer ? [Re: bakenast]
    #25426832 - 08/31/18 07:56 PM (2 years, 8 months ago)

The second pic, the growth on the left side is called tomentose. It is several strains all on top of each other. The ropey looking stuff is called rhyzomorphic growth. Many people seek that out, but in reality it does not matter so much whether its rhyzo or tomentose.

Look at the rusty whyte grows, its mostly tomentose myc and those are beautiful fruits. https://www.shroomery.org/forums/showflat.php/Number/25284782

I usually just let the whole plate colonize and then throw it in the jar since I typically make many plates. I have cut them in quarters before too.

You can try this method -
https://www.shroomery.org/forums/showflat.php/Number/22601023


Goodluck!


--------------------
"Life is warfare and a strangers sojourn, the only lasting fame is oblivion" - Marcus Aurelius



FastFreds Media Cookbook
Mad Seasons guide to hidden contams
How it should and shouldn't look
Ultimate Tek Compendium


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Nataraja_Shiva]
    #25426917 - 08/31/18 08:32 PM (2 years, 8 months ago)

Ok thanks I’ll let it grow it out the rest of the way and do 1/4 per plate they are 100mm plates I’m switching to the no pour mini plate tek, after this  I hate these big things


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleCaps McGeeS
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Agar second transfer ? [Re: bakenast]
    #25426936 - 08/31/18 08:39 PM (2 years, 8 months ago)

I like to make up a 60mm dish for each jar I plan to inoculate, and drop a whole plate to each jar. I've been mixing the agar soft  (7-8g per 500ml of water) and cutting the plates in a tic tac toe pattern, effectively making 9 (and sometimes 16 with 3 vertical and 3 horizontal cuts)wedges ... shaking the hell out of them after SAB session, basically liquefies the agar, and acts  as an LI essentially... full colonization in 5-7 days depending on variety


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineQuirkmeister92
Street Doctor


Registered: 02/01/18
Posts: 6,175
Loc: Grateful Dead USA
Last seen: 16 hours, 31 minutes
Re: Agar second transfer ? [Re: bakenast]
    #25426942 - 08/31/18 08:40 PM (2 years, 8 months ago)

I love me a big ol petri. I get like 6 nice wedges out of each and then g2g each of those if they colonize clean. Super efficient.


--------------------
Team wheat...


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineNataraja_Shiva
Philosophus


Registered: 05/15/18
Posts: 325
Loc: Earth
Last seen: 3 months, 19 days
Re: Agar second transfer ? [Re: Caps McGee]
    #25426986 - 08/31/18 09:02 PM (2 years, 8 months ago)

Quote:

Caps McGee said:
I like to make up a 60mm dish for each jar I plan to inoculate, and drop a whole plate to each jar. I've been mixing the agar soft  (7-8g per 500ml of water) and cutting the plates in a tic tac toe pattern, effectively making 9 (and sometimes 16 with 3 vertical and 3 horizontal cuts)wedges ... shaking the hell out of them after SAB session, basically liquefies the agar, and acts  as an LI essentially... full colonization in 5-7 days depending on variety




:headbang3:
I like that idea!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 1 year, 4 months
Re: Agar second transfer ? [Re: Nataraja_Shiva]
    #25427019 - 08/31/18 09:14 PM (2 years, 8 months ago)

You could always go a bit further and make more transfers to further isolaste.  This won't guarantee a better grow..  But that isnususlly the idea of plates to isolate and find good performers which u can store and come. Back to.

Yoiu don't need much for inoculating jars.  A small wedge is nolenty


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Caps McGee]
    #25427020 - 08/31/18 09:14 PM (2 years, 8 months ago)

man I’ve been using 20g agar to 400ml oat boil water wasting so much of my agar ,,  guess
I need to cut back on that to will last me twice as long. Had no idea so much less works lol


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineQuirkmeister92
Street Doctor


Registered: 02/01/18
Posts: 6,175
Loc: Grateful Dead USA
Last seen: 16 hours, 31 minutes
Re: Agar second transfer ? [Re: bakenast]
    #25427026 - 08/31/18 09:18 PM (2 years, 8 months ago)

Yeah use 1/3 of those packets per 400ml


--------------------
Team wheat...


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineNataraja_Shiva
Philosophus


Registered: 05/15/18
Posts: 325
Loc: Earth
Last seen: 3 months, 19 days
Re: Agar second transfer ? [Re: bakenast]
    #25427037 - 08/31/18 09:21 PM (2 years, 8 months ago)

Damn thats a lot. haha Try between 8-10g per 500ml.

I Do pasty plates, I can make like 20 of them with 1cup(236.588ml) of water and 5g of agar.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Nataraja_Shiva]
    #25427067 - 08/31/18 09:31 PM (2 years, 8 months ago)

Yea so  glad I posted this I killed two birds  it’s wih one stone,  was using this bodhista agar recipe 
” Grain Soak Water agar(save water from hydration of grain spawn add 20g/L Agar-Agar powder””
but for whatever reason I was thinking 20g per 400ml..    Just now realize it’s 20g per liter.  Thanks all for help/advice.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleCaps McGeeS
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Agar second transfer ? [Re: bakenast]
    #25428312 - 09/01/18 01:44 PM (2 years, 8 months ago)

Holy shit... how's that 220% harder agar cut?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Caps McGee]
    #25429396 - 09/01/18 10:19 PM (2 years, 8 months ago)

Lol yea I guess so my take a saw to cut a wedge. Out of it


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineMikect05
Stranger
 User Gallery
Registered: 11/03/17
Posts: 62
Last seen: 1 year, 7 months
Re: Agar second transfer ? [Re: bakenast]
    #25429476 - 09/01/18 11:11 PM (2 years, 8 months ago)

How do you like the grain water agar?  I have been using PDA, but thinking about making grain water agar and throwing a couple hardwood pellets in some for my wood lovers


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 917
Last seen: 3 months, 16 days
Re: Agar second transfer ? [Re: Mikect05]
    #25429945 - 09/02/18 07:24 AM (2 years, 8 months ago)

The only other mix I have used is LME and the only different I can tell is the tint of it, growth is same for me


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Kratom Powder For Sale   Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, Agar, Petri Dish, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* agar transfers buggers 3,722 12 11/03/07 12:44 PM
by orchidfanatic
* agar transfer substitution? omniscientlore 1,189 1 10/04/04 12:32 AM
by ATWAR
* How to make agar 2 agar transfer? esp 5,007 7 01/08/04 01:08 PM
by YidakiMan
* agar to agar transfer ohmatic 10,403 11 01/12/21 11:00 PM
by bodhisatta
* agar transfers *DELETED* thisone 1,055 3 11/20/03 10:58 PM
by Mycomancer
* did i do my agar to agar transfer right? *DELETED* vinzy_a 961 2 09/29/06 07:05 AM
by vinzy_a
* substitute for agar? brewwhaha1 1,844 15 02/19/05 11:27 PM
by MontyRev
* agar to agar / g2g degeneration effects ohmatic 2,080 11 01/17/05 05:46 PM
by MushroomFriend

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
878 topic views. 44 members, 237 guests and 93 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 13 queries.