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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Noob + Agar
    #25419986 - 08/28/18 06:56 PM (6 years, 4 months ago)

After months of creeping here, I decided to start my grow with pasty plates and a spore print. Followed the TEK to a T, and to my dismay, I'm 13 days in with no mycelium growth. I'm guessing my agar might be too firm. Noob mistake.

I also jumped the gun and already pressure cooked oats today after thinking I saw some growth through my SAB. I still have some of the spore print leftovers but fear they may be more prone to contamination the second time around.


Any help would be much appreciated, I have a few Q's:

Should I cut my losses with these plates and start over, or wait it out a bit longer?

Could I tiger drop one of the plates pre-growth into grains and just see what happens?

What's my best bet for getting a clean culture without having to order more spores?

Thank you kindly x




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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
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Re: Noob + Agar [Re: fungurl]
    #25419992 - 08/28/18 06:58 PM (6 years, 4 months ago)

Spores can take up to 6 weeks to germinate... I'd say be patient,  and start some new as well... I find softer agar to speed things up a bit:  especially with old/dry spores... you could drop it, but it'd be a crapshoot... wait on germination, transfer the first confirmed mycelial growth... let that grow to the size of a dime or nickel, and transfer again, from the cleanest, most rhizomorphic  (ropey) growth available... repeat until you have a  plate uniform both in growth, and shape


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OfflineVroomerMcZoomers
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Registered: 08/11/17
Posts: 1,637
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Re: Noob + Agar [Re: fungurl]
    #25420055 - 08/28/18 07:26 PM (6 years, 4 months ago)

Did you order from a Canadian vendor that rhymes with Whore swan oh whan?  If you did, that's why there is no growth. 

13 days is a long time.  Ive never had to wait that long.  What temps are you keeping them at?  Grow guides I read back in the age of books would say to incubate newly inoculated plates at 88F for 24 to 48 hours to get the spores to break dormancy. 

I still do this and I always get growth in less than a week, except for that time I tried Whore swan oh whan.

*Edited to remove a vendors name.


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

Edited by VroomerMcZoomers (08/28/18 07:31 PM)

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InvisibleCaps McGee
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Re: Noob + Agar [Re: VroomerMcZoomers]
    #25420057 - 08/28/18 07:27 PM (6 years, 4 months ago)

Interesting about the temps, I'd remove vendor... A private message perhaps?


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OfflineVroomerMcZoomers
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Re: Noob + Agar [Re: Caps McGee]
    #25420062 - 08/28/18 07:29 PM (6 years, 4 months ago)

I thought that only applied to shroomery sponsors


--------------------
 

Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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InvisibleCaps McGee
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Re: Noob + Agar [Re: VroomerMcZoomers]
    #25420313 - 08/28/18 09:04 PM (6 years, 4 months ago)

Idk... i would think if anything, sponsors would be the exception...


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: Noob + Agar [Re: Caps McGee] * 1
    #25420333 - 08/28/18 09:11 PM (6 years, 4 months ago)

ralphsters and spores101 are scams, don't mention actual sponsors in the psychedelic section(this one)

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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: Caps McGee]
    #25420525 - 08/28/18 11:47 PM (6 years, 4 months ago)

Thanks for the tip. I guess I'll keep em around :smile: Making some softer plates as we speak

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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: VroomerMcZoomers]
    #25420530 - 08/28/18 11:52 PM (6 years, 4 months ago)

I ordered from a reputable seller on reddit. Kept plates in my garage, and temperatures hit 85-90 outside, so I imagine they're nice and warm especially during the day :sun:

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InvisibleCaps McGee
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Re: Noob + Agar [Re: fungurl]
    #25420703 - 08/29/18 05:18 AM (6 years, 4 months ago)

That's hot!they'll do fine 65-70


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OfflineBLINKfan420
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I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 6 days
Re: Noob + Agar [Re: Caps McGee] * 1
    #25420945 - 08/29/18 08:55 AM (6 years, 4 months ago)

I thought we were past that "mycelium does better at higher temps" stuff.  Isn't the age of incubation and heating pads done and over with now? Also, higher temps are better breeding grounds for bacteria.

Room Temperature FTW

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.

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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Noob + Agar [Re: BLINKfan420]
    #25421019 - 08/29/18 09:27 AM (6 years, 4 months ago)

Yeah... I never get above 73

Love the edit vroomer! :rofl:


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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: fungurl]
    #25611926 - 11/13/18 12:06 PM (6 years, 2 months ago)

2 failed attempts at agar, I figure 3rd time's the charm, right? I hate that I'm wasting so much foil and paper towels. Can I recycle them for use on my next plates?

Also, this batch came with heavy condensation even when covering with paper towels post-pour, waiting until hardened to put lids on, and slowly lowering pressure in PC. I put one drop of MS on each of them this morning. Would it help putting them by a heater, getting some indirect warmth? It's 65 degrees in my house currently.




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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Noob + Agar [Re: fungurl]
    #25612303 - 11/13/18 03:10 PM (6 years, 2 months ago)

You can drill holes on the side just under the lip, cover with micropore tape or stuff with polyfil, and skip the foil on most (still foil bottom stack perhaps)


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Noob + Agar [Re: Caps McGee]
    #25612346 - 11/13/18 03:33 PM (6 years, 2 months ago)

I reuse the same foil/paper towels. I just keep using them till they ripped or whatever and I can’t use them anymore. You sure got a lot of plates in that SAB! Looks a bit crowded.
Patience is so important when it comes to this stuff. I can’t stress that enough lol.


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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: Caps McGee]
    #25612382 - 11/13/18 03:52 PM (6 years, 2 months ago)

Thanks for the tip! I’ll do that for my next run.

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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: Buck513]
    #25612412 - 11/13/18 04:06 PM (6 years, 2 months ago)

My first few plates never germinated, so I made more containers for a second try. Patiently waited and all of them were duds, so I figured this time I’d make a surplus of plates and innoc half of em with new spores and store some extras for if (when) I fuck it up again 😊

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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Noob + Agar [Re: fungurl]
    #25612434 - 11/13/18 04:17 PM (6 years, 2 months ago)

Failure happens: only way to get better, and best way to learn

Also why it's a good idea to have multiple projects at different stages


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Offlinefungurl

Registered: 06/04/18
Posts: 23
Last seen: 2 years, 4 months
Re: Noob + Agar [Re: Caps McGee]
    #25613013 - 11/13/18 08:35 PM (6 years, 2 months ago)

I like your perspective :wink:

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InvisibleTedsDead
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Registered: 01/03/17
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Re: Noob + Agar [Re: fungurl]
    #25613040 - 11/13/18 08:47 PM (6 years, 2 months ago)

Quote:

fungurl said:
2 failed attempts at agar, I figure 3rd time's the charm, right? I hate that I'm wasting so much foil and paper towels. Can I recycle them for use on my next plates?





I use a kitchen towel and a diner plate works over and over no trash


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

Edited by TedsDead (11/13/18 08:48 PM)

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