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OfflineHiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado Flag
Last seen: 3 years, 3 months
Fully colonized grain Spawn to Liquid Culture
    #25389985 - 08/15/18 11:26 AM (5 years, 5 months ago)

So I went from a clone to lc, then used some of the lc to make grain spawn. Can I got from grain spawn and make a new lc? Pretty sure I can just break up the fully colonized grain spawn and fill it with water and then suck it back out and have a new LC right?


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"Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about."

- Rumi


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OfflineHiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado Flag
Last seen: 3 years, 3 months
Re: Fully colonized grain Spawn to Liquid Culture [Re: LtLurker]
    #25390467 - 08/15/18 03:38 PM (5 years, 5 months ago)

Im not going G2G because I use bags with injection ports and I use LC. I'm not doing agar because its a clone. I either use a clone or a isolate. Not both. Clone vs Isolate is basically the same thing just from different ends but lets stay on topic. The clone performs very well, I dont need to grow it out on agar. I also use LC because I can inoculate each 6 quart bag of rye grain with 20cc's of LC and its done in less than a week. My simple question was could I go from colonized grain to LC!?!?! LOL I didn't pull this idea out of my ass either. Somebody had posted about it a long time ago. Somebody knowledgeable in the mycology field. I guess i'll see if I can find it.


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"Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about."

- Rumi


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OfflineHiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado Flag
Last seen: 3 years, 3 months
Re: Fully colonized grain Spawn to Liquid Culture [Re: SpikeSpiegel]
    #25392028 - 08/16/18 10:09 AM (5 years, 5 months ago)

Quote:

SpikeSpiegel said:
Quote:

HiLIFE said:
Im not going G2G because I use bags with injection ports and I use LC. I'm not doing agar because its a clone. I either use a clone or a isolate. Not both. Clone vs Isolate is basically the same thing just from different ends but lets stay on topic. The clone performs very well, I dont need to grow it out on agar.




:okiapprove:

Personally I would't do what you're thinking (and a good argument for agar) because IMO there's something sketchy about grain floating around in nutrient-rich liquid for days. I'm sure it can be and has been done. If you've lost spawn to dirty LC before though you'll appreciate others' words of warning, that's all.

I think I get what you're trying to say about a clone not needing agar but I don't think that's the point people are making. The point is that agar is a good route to arrive at clean LC, not because you're after an isolate or trying to find better performing myc.

And what does LC and injection ports have to with G2G? You can still do it. Or you don't want to?



I dont have a flowhood so I dont do g2g!


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OfflineHiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado Flag
Last seen: 3 years, 3 months
Re: Fully colonized grain Spawn to Liquid Culture [Re: scifipirate]
    #25392032 - 08/16/18 10:12 AM (5 years, 5 months ago)

Quote:

scifipirate said:
Quote:

HiLIFE said: Pretty sure I can just break up the fully colonized grain spawn and fill it with water and then suck it back out and have a new LC right?



This is correct, go for it. Used to be a pretty standard way of making LCs, guess it's lost popularity...




So basically what I ended up doing was putting 60ml of sterilized LC into the jar, and then sucked up as much as I could back out and added back into the LC jar where it should grow to a new LC that i'm sure is clean. As soon as I get a flowhood i'll be doing g2g but until then. I feel like LC is just so easy though.


--------------------
"Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about."

- Rumi


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