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OfflineSpikeSpiegel
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Registered: 07/30/18
Posts: 162
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Re: Fully colonized grain Spawn to Liquid Culture [Re: HiLIFE]
    #25390700 - 08/15/18 05:33 PM (5 years, 5 months ago)

Quote:

HiLIFE said:
Im not going G2G because I use bags with injection ports and I use LC. I'm not doing agar because its a clone. I either use a clone or a isolate. Not both. Clone vs Isolate is basically the same thing just from different ends but lets stay on topic. The clone performs very well, I dont need to grow it out on agar.




:okiapprove:

Personally I would't do what you're thinking (and a good argument for agar) because IMO there's something sketchy about grain floating around in nutrient-rich liquid for days. I'm sure it can be and has been done. If you've lost spawn to dirty LC before though you'll appreciate others' words of warning, that's all.

I think I get what you're trying to say about a clone not needing agar but I don't think that's the point people are making. The point is that agar is a good route to arrive at clean LC, not because you're after an isolate or trying to find better performing myc.

And what does LC and injection ports have to with G2G? You can still do it. Or you don't want to?


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