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Shop: Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies, Injection Grain Bag

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OfflineLtLurker
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Re: Fully colonized grain Spawn to Liquid Culture [Re: HiLIFE]
    #25390000 - 08/15/18 11:31 AM (5 years, 5 months ago)

clean it up on agar first.


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Re: Fully colonized grain Spawn to Liquid Culture [Re: Caps McGee]
    #25390024 - 08/15/18 11:39 AM (5 years, 5 months ago)

Quote:

Caps McGee said:
Why not G2G?



That's a way better response. Why add the extra step(and contam vector) at all unless you're gonna clean it up a bit first?


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Re: Fully colonized grain Spawn to Liquid Culture [Re: HiLIFE]
    #25390488 - 08/15/18 03:45 PM (5 years, 5 months ago)

What do you think the definitions for clones and isolates are? I think you're confused. You use agar for both of those things.

Clean culture on agar(whether it's ms, clone or isolate) is used to make lc. I think you're just gonna make a mess trying to make an LI like you described.


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Re: Fully colonized grain Spawn to Liquid Culture [Re: HiLIFE]
    #25392038 - 08/16/18 10:15 AM (5 years, 5 months ago)

You can g2g in a SAB just fine. Same for agar, which is way cleaner as we all keep telling you.

:whiteflag: on the rest. Can't make heads or tails of half what you're saying and dumping water into a grain bag then sucking it up doesn't sound clean at all. GL hope it works out for ya.


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Re: Fully colonized grain Spawn to Liquid Culture [Re: FishLevelMidnight]
    #25392091 - 08/16/18 10:36 AM (5 years, 5 months ago)

I get it used to be more common, but lots of things have fallen by the way side cause they just aren't as good as the methods more common now.

Most grain jars/bags that i've seen aren't 100% clean. Seems like adding/drawing water is going to practically guarantee picking up some contams, then you're gonna put them into an lc where contams thrive.

Now, there doesn't seems to be a good reason to do it that way since g2g (or a2lc) is cleaner, faster, and doesn't require different equipment. Unless you're just having some fun and failure is ok.

but of course, it's just :2cents: and he seems intent on going this route, so I give and hope it works out alright for him.


Edited by LtLurker (08/16/18 10:37 AM)


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