Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, pH Test Strips

Jump to first unread post. Pages: 1
Offlinemethikist
Mushroom Pirate.

Registered: 03/16/04
Posts: 138
Loc: PNW
Last seen: 11 years, 7 months
Cubensis Question.
    #2537905 - 04/08/04 03:03 AM (12 years, 7 months ago)

Is the species septate or coenocytic? I'm doing some experiments, need a quick answer.


--------------------
Oh cmon, I'm a fun guy.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemethikist
Mushroom Pirate.

Registered: 03/16/04
Posts: 138
Loc: PNW
Last seen: 11 years, 7 months
Re: Cubensis Question. [Re: methikist]
    #2537960 - 04/08/04 03:27 AM (12 years, 7 months ago)

I'm almost positive they are septate, which leads me to the next question. What are the specific and ideal conditions to trigger plasmogamy between counterpart mating type hypha. I'm going to take some cubensis gametes to the lab and test a whole slew of conditions. I'll post my results.


--------------------
Oh cmon, I'm a fun guy.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Cubensis Question. [Re: methikist]
    #2538217 - 04/08/04 06:53 AM (12 years, 7 months ago)

I would post it in advanced


--------------------
Sr_Setahongo


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Cubensis Question. [Re: methikist]
    #2540735 - 04/08/04 09:06 PM (12 years, 7 months ago)

The compatability is built into the genes of each monokaryon. All vegetative growth(which plasmogamy would fall under) occur within a range of temps/humidities/ph/nutritional composition of substrate. Optimal will vary from monokaryon to monokaryon. Proximity and compatability are the most significant factors effecting plasmogamy. Monokaryons also express different rates and type of growth based on there gentic makeup, on any media formulation. A specific monokaryon might grow really slow on any formulation you come up with, in relation to other monokaryons on any formulation. This is why proximity is so important. Fast growing A1B1 might only succesfully mate with very slow growing A2B2, so proximity is very important.

Their septate. With clamp connections present upon plasmogamy. If your are going to make the effort, please try some compatability studies between Different Strains of cubensis. Try and mate monokaryons of say GT with EQ, Mexicube, etc... At this point we need some conformation as to what mates with what, and possible determination of ancestory of all the strains.

Plasmogamy is going to occur at a rapid rate as long as the conditions are condusive to growth( wide range). Optimization of any vegetative growth is good enough, you don't have to isolate monokaryons to optimize a media for rapid growth.

EDIT.

It has been observed in other species that compatability can be structural/physiological in nature, so altering some enviromnmental parameter might break down that structural/physiological barrier. So if you really have your heart set on experimneting with this, go for it. I would imagine that compatability barriers exist for good reason. I don't think that is what you are talking about though. I think you are just asking about optimizing Growth during Plasmogamy, which would fall under Agar formulation optimization, and you probably don't have to isolate monokaryons to do that. Just judge the speed of growth from spore germination to first transfer on differing media formulations.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemethikist
Mushroom Pirate.

Registered: 03/16/04
Posts: 138
Loc: PNW
Last seen: 11 years, 7 months
Re: Cubensis Question. [Re: ]
    #2541126 - 04/08/04 10:50 PM (12 years, 7 months ago)

Interesting, thanks for the information Teonan. I understand what you mean about the diversity that monokaryons have, after all they are only gametes, so any apparent mutations are going to be evident right off the bat. With enough spores, and enough media with the right conditions, I don't see it being very hard to introduce 2 foreign monokaryons successfully, if such event happened, I would most likely isolate it immediately, incubate accordingly and transfer the mycelia to an appropriate substrate, thus creating a cross breed of mushroom. Totally interesting, I'm thrilled.. Off to the lab


--------------------
Oh cmon, I'm a fun guy.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKillBill
Quick Learner
Registered: 07/02/04
Posts: 109
Last seen: 12 years, 1 month
Re: Cubensis Question. [Re: methikist]
    #2947135 - 07/31/04 02:51 AM (12 years, 4 months ago)

*drools*


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Cubensis Question. [Re: methikist]
    #2947593 - 07/31/04 08:25 AM (12 years, 4 months ago)

>I don't see it being very hard to introduce 2 foreign monokaryons successfully,[...]

You mean a simple mating experiment? Yea, that's not difficult at all.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* dikaryotic mycelium questions LochRaven 3,730 18 05/05/06 03:02 PM
by texas34
* another genetic question. Mindzpore 1,503 9 05/30/05 10:43 AM
by Mindzpore
* Hypothetical Genetic Question
( 1 2 all )
tahoe 3,022 30 05/04/05 07:17 PM
by EonTan
* sexual reproduction (Cubensis) sublimemushhead 2,424 17 09/12/07 09:45 PM
by fastfred
* The Return of the Red Spore Cubensis
( 1 2 3 4 5 6 all )
RogerRabbitM 17,914 104 05/10/10 05:05 PM
by total
* Multispore Mycology question Flashman 666 3 01/18/05 07:04 AM
by Flashman
* Cubensis Cross Breeding?
( 1 2 all )
shroomsynergy 8,746 36 10/24/14 04:31 AM
by Edmunter
* Monokaryon Identification Noj 1,736 2 02/16/07 12:22 PM
by Workman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
957 topic views. 1 members, 3 guests and 3 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.041 seconds spending 0.004 seconds on 14 queries.