reason for contam. - Mushroom Cultivation

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orangeboy
rick jamesbitch.

Registered: 03/17/04
Posts: 22
Last seen: 18 years, 9 months

reason for contam.

#2531805 - 04/06/04 02:13 PM (18 years, 11 months ago)

So 4 of my BRF Verm jars are contaminated as I just noticed after 7 days of being in the incubator. I was just wondering if the incubator could of caused the contamination to form? I am very doubtful that it was my sterlization proccess while insolating but who knows. I was wondering if it could have came from the incubator itself if it was unclean? My dumb ass used a tupperwear box from my basement that has been down there for a few years.. It smells like sour apples and looks like greyish/brown clumps forming on the cake.. eh I beleive it is sour rot, but what exactly causes this? Thanks

Edited by orangeboy (04/06/04 03:40 PM)

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Anno
Experimenter

Registered: 06/17/99
Posts: 24,156
Loc: my room
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Re: reason for contam.

[Re: orangeboy]
#2532314 - 04/06/04 04:20 PM (18 years, 11 months ago)

What was your means of sterilisation? For how long?

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orangeboy
rick jamesbitch.

Registered: 03/17/04
Posts: 22
Last seen: 18 years, 9 months

Re: reason for contam.

[Re: orangeboy]
#2533367 - 04/06/04 10:27 PM (18 years, 11 months ago)

I dont have a pressure cooker so i used a big pot with a tight lid. And I boiled for about an hour and 15 minutes.

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Im one of the baddest mother fuckers of all time. I'm one of the best singers and best looking mother fuckers u've ever seen. Hold my drink bitch.

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Anonymous

Re: reason for contam.

[Re: orangeboy]
#2533717 - 04/07/04 12:41 AM (18 years, 11 months ago)

Post deleted by user's request.

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Suntzu
Geek

Registered: 10/14/99
Posts: 1,396
Last seen: 9 months, 16 days

Re: reason for contam.

[Re: orangeboy]
#2534237 - 04/07/04 08:29 AM (18 years, 11 months ago)

It's a gamble whether you're going to get complete sterilization of BRF jars without a pressure cooker. The temps are just not getting high enough to be sure.
I think I remember someone microwaving the hell out of BRF, then boiling it as you described. . .they were contam-free. The problem with this is the potential water loss. I'd really recommend making a stop or two at some thrift stores for a small 'presto' type PC. At least here, I always see a few around, very cheap. Even if you can only get up to 5-10 PSI, it will help in a big way.
You're description of the contam sounds like textbook Bacillus, the endospore-producing bugger that plagues incompletely sterilized grain.
Good luck;

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MAIA
World-BridgerKartikeya (DftS)

Registered: 04/27/01
Posts: 7,377
Loc: Erra - 20 Tauri - M45 Sta...
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Re: reason for contam.

[Re: orangeboy]
#2534356 - 04/07/04 09:06 AM (18 years, 11 months ago)

Read the faq about fractional sterilization. I find it the safest method if you don't have a PC.

MAIA

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orangeboy
rick jamesbitch.

Registered: 03/17/04
Posts: 22
Last seen: 18 years, 9 months

Re: reason for contam.

[Re: MAIA]
#2537385 - 04/07/04 10:24 PM (18 years, 11 months ago)

thanks for the help

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Im one of the baddest mother fuckers of all time. I'm one of the best singers and best looking mother fuckers u've ever seen. Hold my drink bitch.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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