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OfflineCthulhu23
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PF Tek troubleshooting * 1
    #25346554 - 07/25/18 04:36 PM (5 years, 6 months ago)

Hi all,  First, I want to say THANK YOU to such a great community.  Just by reading through everyone's posts I have learned a TON.

This is my first grow experience.  I've made numerous mistakes (and learned what not to do in the process!), but I am at a bit of a crossroads and would like some help.

I have several BRF cakes that are still colonizing, have been waiting for them to get fully colonized for ~4 weeks now.  After being gone for 1 whole day, I have around 6 jars that are only 1/4 to 1/2 colonized on the upper half of the jar.  I suspect the bottom half are too wet (one of my mistakes was thinking more of a spore syringe was better, than less, and for 12 jars I used 2 syringes!) They seem to refuse to colonize the bottom half of the jar.  Three of these are putting out pins!  I also have two jars that are contaminated (black/bluish mold looking stuff). 


Pins!


Going strong!


Meh...

My questions:
1) For the pinning cakes that aren't fully colonized, should I wait, or birth them now?
2) For the contaminated cakes, I assume I just toss; or is there some way to salvage them (i.e., birth, remove contams, then dunk)?

I'll add pics shortly.  Incidentally, I have 1 jar that is going strong - about 75% colonized (started at the bottom!) and I'm crossing my fingers it doesn't contam out like the other two jars that did (and that looked great until today).


Edited by Cthulhu23 (07/25/18 05:04 PM)


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25346699 - 07/25/18 06:10 PM (5 years, 6 months ago)

I may have my answer for the pinning before full colonization: I see some spots on the bottom of the jar, maybe 3-5mm in diameter, that are yellowish.  Bacterial contamination?  Would explain why the other jars wouldn't continue colonizing downward.

Fingers crossed the single "good" one I have left completes!


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OfflineANJ245
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25346733 - 07/25/18 06:25 PM (5 years, 6 months ago)

I am glad to step up and brainstorm with you.  I too am on my first grow. I believe that we started our called about the same day. 

Mine were started on June 19 they are starting to pin now.  One was invitro it didn't get dunked, straight to the SGFC. I have 1 flush from it.

I have a second set of 5 jars started on July 4. They just went to the SGFC yesterday.

The third set is going faster.

I have pitched three jars because of contamination.  Two of the three had the bluish looking stuff that you have.  I couldn't help myself, I dissected it.  The contamination gets much worse the deeper you look. 

The idea of keeping them was a no go. Just like this community says.

Just curious, when did you start your cakes?


--------------------
Good help is hard to find.
I shit you not.


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: ANJ245]
    #25346819 - 07/25/18 07:14 PM (5 years, 6 months ago)

Thanks, ANJ245!

I inoculated 12 cakes with B+ on June 29. 

made many mistakes!  I used the Simple Minds TEK because I hadn't found this site yet.  So different BRF/Verm ratio.  Then, I inoculated in open air.  :shocked:I also used 91% iso, and wiped the needle on the syringe with it after flame sterilizing.  So, I'm not exactly surprised I have contamination.

Another problem I think is that I've been keeping the jars in my closet.  It's been pretty warm here recently and I have the AC turned down turn the day when I'm gone at work.  I bet it's getting close to 80 in there.

I've read that jars pin prior to 100% colonization in response to contamination.  I've also read that some people have successfully cut off the non colonized portion and put it into fruiting.  I'm thinking about experimenting and trying that out.

I plan to fruit in a hybrid monotub!


Edited by Cthulhu23 (07/25/18 07:15 PM)


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OfflineVroomerMcZoomers
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25347065 - 07/25/18 09:22 PM (5 years, 6 months ago)

Do you have a gas exchange system in those lids?  (synthetic filter disk)

If not that could be why they are having trouble.

As for the contaminated cakes, there is pretty much no way to deal with that.  They are garbage.

The ones that are pinning, I think you should birth them but expect to only get that one flush off em.  Try to separate the non colonized part and keep your eyes peeled for contams.

Don't worry about those yellow drops.  That's just excrement.


--------------------
 

Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky


Edited by VroomerMcZoomers (07/25/18 09:23 PM)


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Re: PF Tek troubleshooting [Re: VroomerMcZoomers]
    #25347245 - 07/25/18 11:54 PM (5 years, 6 months ago)

Turn the jars that are pinning but not contaminated upside down, then slam them down on a hard surface like a table.  it will break the cake away from the glass on the bottom, and hopefully allow a little easier colonization.  leave them from this point on upside down.  RR has suggested it, and i've done it a few times.  Take a marker and outline where the myc line is all the way around the jar, and that way you can see if it is still colonizing.  If it doesn't move at all after a week, then you can experiment with birthing it uncolonized, etc.


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.


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OfflineVroomerMcZoomers
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Re: PF Tek troubleshooting [Re: naturalistic123]
    #25347278 - 07/26/18 12:45 AM (5 years, 6 months ago)

That's a great idea for stalled colonization, but the jar is pinning. 

If it's birthed now, it's pretty much a slam dunk that OP will get some mushies, the other way may or may not work, and will block the flush.


--------------------
 

Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky


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Re: PF Tek troubleshooting [Re: VroomerMcZoomers]
    #25347283 - 07/26/18 12:51 AM (5 years, 6 months ago)

Well I can only speak from my own experience.  My jars were pinning as well, harder than OP's.  I myself just waited it out, birthed when fully colonized, picked the mushrooms that had grown almost to maturity, then fruited, and they seemed to do just fine.  Maybe I would have gotten more had I birthed instead of waiting.


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.


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OfflineLogicaL ChaosM
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25347342 - 07/26/18 02:50 AM (5 years, 6 months ago)

Looks like your jars were too fluffy and split off so to speak.

So the mycelium thinks the cake ends at the half way point when it doesnt.

Next time, pack the PF mixture gently! Do no pack tight, but enough to make a cake if that makes sense.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: LogicaL Chaos] * 1
    #25347520 - 07/26/18 06:58 AM (5 years, 6 months ago)

Thanks for the responses, everyone!

Vroomer - to answer your question I just used a dry verm layer at the top of the jar for my filtration. 

I birthed the cakes last night.  Jars smelled like mushrooms, so I’m guessing not to a of contamination.

The bottom half of the BRF/verm was GOOEY.  Mycelium has colonized the top dry verm layer!  I suspect it was just too wet for the mycelium to colonize.  I don’t know what a consolidated cake is supposed to look/feel like but what I have felt firm and solid.

The cakes are soaking in water today.  I’ll be rolling them in verm and fruiting tonight.  I’ll post pics if they flush!


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25353857 - 07/29/18 11:41 AM (5 years, 6 months ago)

Some updates:

I decided to take the pins that were visible for cloning (I was paranoid about not having any shroomies given my contamination fears).  First clone to agar!



I also wondered if I could get your guys opinion on my FC conditions?




I have a few more pins!


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: Cthulhu23] * 1
    #25370833 - 08/06/18 06:34 PM (5 years, 6 months ago)

I got a few mushrooms!  Second flush starting and first flush on a few other sad little cakes...but it was a good start and great learning experience!



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InvisibleMateja
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25370894 - 08/06/18 07:09 PM (5 years, 6 months ago)

Link is in ny sig if you want more mushrooms next time you fruit cakes :wink:


--------------------
Cakes inside Water Tub


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: Mateja]
    #25370921 - 08/06/18 07:19 PM (5 years, 6 months ago)

Mateah I actually did EXACTLY that last night after seeing your post a few days ago!  So, wish me luck with the 2nd flush, and then 12 more cakes of AA+ in a few weeks :laugh:

And thank you for the advice and TEK!!


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InvisibleMateja
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Re: PF Tek troubleshooting [Re: Cthulhu23]
    #25371063 - 08/06/18 08:18 PM (5 years, 6 months ago)

:super: and don't forget to post results in the Humidity Chamber thread! I will update the OP with everyone's results :eatingout:


--------------------
Cakes inside Water Tub


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OfflineMH5109
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Re: PF Tek troubleshooting [Re: Mateja]
    #25371109 - 08/06/18 08:43 PM (5 years, 6 months ago)

Quote:

Mateah said:
:super: and don't forget to post results in the Humidity Chamber thread! I will update the OP with everyone's results :eatingout:




I have about 36 cakes colonizing right now I'll have to see about trying your setup too.  Looks easier than the one I have going now.


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InvisibleMateja
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Re: PF Tek troubleshooting [Re: MH5109]
    #25371122 - 08/06/18 08:51 PM (5 years, 6 months ago)

I'd hate to see 36 bruised cakes in a SGFC haha cakes love the Humidity Chamber


--------------------
Cakes inside Water Tub


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OfflineApc123
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Re: PF Tek troubleshooting [Re: Mateja]
    #25371364 - 08/06/18 10:48 PM (5 years, 6 months ago)

The first time I did pf tek I had the same problem with the bottom part of my jars not colonizing because of wetness. I eventually just birthed them, put them in water and carefully washed away the uncolonised parts and then just continued as normal and it worked just fine.


--------------------
The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.


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InvisibleZiran
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Re: PF Tek troubleshooting [Re: Apc123]
    #25371482 - 08/07/18 12:43 AM (5 years, 6 months ago)

those mushrooms look over saturated. I'd back off of misting.


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OfflineCthulhu23
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Re: PF Tek troubleshooting [Re: Ziran]
    #25372539 - 08/07/18 01:14 PM (5 years, 6 months ago)

Thanks guys.  Yep definitely over saturated (fuzzy feet).  Then too dry.  I think I’ve got things figured out now though.  The humidity chamber looks to be a lot better deal than my hybrid monotub.  As a matter of fact several of those baby shroomies were open this morning so I picked em!


Edited by Cthulhu23 (08/07/18 01:15 PM)


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