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OfflineFishLevelMidnight
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PC bags without sealing * 3
    #25367454 - 08/05/18 12:42 AM (5 years, 6 months ago)

When I started using bags I was a little paranoid about getting water in, not having sterile substrate, and ruining the bags when I pulled them out of the PC. I was using zipties with polyfil, zipties, using a food sealer... it was too much work and costs and a pain to undo them when trying to spawn.

With help from others here and a little trial and error, I've developed a method for doing filter patch bags where the folding method allows them to self seal and stay sterile even when handled some. The bags I’ve used are 5 um, but I can’t image that 0.2 um wouldn’t work. I’ll give it a try if someone wants to sent a few bags :P or post results here!

I'll do my best to explain and I took some photos which are below. Hope it helps!

Basically I do the following:

1. Position bag with filter patch facing away from body. (Figure 1, Panel 1)

2. Fold gussets sides in, pinch the top of the substrate at gussets and make sure it folds properly all the way to the top.

3. Turn the substrate towards you while pinching at the top (one hand example Figure 1, Panel 2), in this way you are folding the substrate on top of the gusseted top (Figure 1, Panel 3).

4. Fold the top of the bag so it covers the filter patch (Figure 1, Panel 4). I like to gently tuck it underneath the bag.

5. Continue to roll the bag forward such that it sits on top of the fold that is on top of the patch, press lightly (Figure 1, Panel 5)



6. When loading in the PC, the bottom bags should be placed such that the folded top sits along the side of the PC (Figure 2, Panel 6). I do not use towels or anything in between them.

7. The bags that are not slightly in water can be placed filter patch down (Figure 2, Panel 7). I always add in some containers of water or instruments or LC to PC while I do bags, might as well fill up the space if you have it!

8. The bags will seal after PCing, even if you open hot. They look nicer if you let the PC cool overnight (Figure 2, Panel 8).

9. The bags have sealed together (Figure 2, Panel 9). Sometimes water can get between the folds (not actually in the bag) so be weary when unraveling. Additionally, the fold will sometimes stick to the filter patch, so it is wise to carefully peel back the fold and hold the corner of the patch down so it doesn't stick (or else you can rip it and compromise the filter).



This method has worked with me with lots of success. There was a time where I was PCing bags and then loading into canvas bags and bringing to the flowhood at work, so the seal is durable and can withstand some movement and handling. When you first start you may have the occasional bag that will fill with water, just be weary of how you load the bottom bags and don't overfill (I load mine to the minimum line, 23 qt presto, and run it for 2.75 hours at 20 psi for reference).

I hope this method works for others and this little TEK helps out members here. Mush love.


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Edited by FishLevelMidnight (08/05/18 01:11 AM)


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Offlineverum subsequentis
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Re: PC bags without sealing [Re: FishLevelMidnight]
    #25367469 - 08/05/18 12:56 AM (5 years, 6 months ago)

right on man, nice write up. do you use .2 or .5 micron bags? i'm sure it'd work with either but you may want to add that. maybe not but i think it may help many a noob. you know what it was like to be new. every detail not mentioned in the tek was something to trip on. at least it was for me.


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OfflineFishLevelMidnight
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Re: PC bags without sealing [Re: verum subsequentis]
    #25367479 - 08/05/18 01:10 AM (5 years, 6 months ago)

Good point bud. Mine are 5 um I believe. Always looking for tips!

Btw, I’m in straight awe of where the PE iso project has come.
:hatsoff: :manofapproval:


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Offlineverum subsequentis
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Re: PC bags without sealing [Re: FishLevelMidnight]
    #25367482 - 08/05/18 01:15 AM (5 years, 6 months ago)

thanks man. it's been a journey for sure. we'll see how it goes. a TC once told me as a noob that only 1 in 100 isolates would be any good and i only have 60 or so. RR once said that 1 in 12 would be keepers so we'll see.


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Invisiblettching8475
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Re: PC bags without sealing [Re: verum subsequentis]
    #25367673 - 08/05/18 07:13 AM (5 years, 6 months ago)

:takingnotes:


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InvisibleEyeOnEmitter
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Re: PC bags without sealing [Re: ttching8475]
    #25368647 - 08/05/18 05:04 PM (5 years, 6 months ago)

Awesome Tek!
Yeah, also following the PE isolation thread. Great stuff!
So, I have what look like the same bags and some spawn growing clean.(i think)
Curious as to how you inoculate the bags..
LC?


Edited by EyeOnEmitter (08/05/18 05:05 PM)


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OfflineFishLevelMidnight
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Re: PC bags without sealing [Re: EyeOnEmitter] * 1
    #25368932 - 08/05/18 07:57 PM (5 years, 6 months ago)

You can unfold the top, it seals enough to not contaminate but can be peeled open.

I inoculate with grains is usually, but I’ve done agar with grain in the bag. LC directly injected into the bag has worked for me with grains :smile:


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InvisibleJHOVA
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Re: PC bags without sealing [Re: FishLevelMidnight]
    #25543498 - 10/17/18 12:31 AM (5 years, 3 months ago)

how many 4 quart large .05 bags can you fit in your 23 quart presto? nice writeup!


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OfflineSHROOMSISAY01M
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Re: PC bags without sealing [Re: JHOVA]
    #25543529 - 10/17/18 12:57 AM (5 years, 3 months ago)

You may want to mention the only reason you don't have spacers or a towel to keep the bags away for the side of the PC is you have an electric stove a gas stove will melt the bags. People that don't know that fact and have a gas stove will make a mess ruin bags and sub.


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Invisiblevan hattonFacebookDiscord
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Re: PC bags without sealing [Re: JHOVA]
    #25543589 - 10/17/18 01:58 AM (5 years, 3 months ago)

It will only melt the bags if the flames go up the side of the pc.

Quote:

JHOVA said:
how many 4 quart large .05 bags can you fit in your 23 quart presto? nice writeup!




I can fit four 5lb bags in my prestos. I could probably fit more but no need.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJHOVA
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Re: PC bags without sealing [Re: van hatton]
    #25543846 - 10/17/18 07:12 AM (5 years, 3 months ago)

Dope. I have the baby presto too maybe that one can fit 2 bags?

How long do you pc these mofos for?


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InvisibleTookitooki
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Re: PC bags without sealing [Re: JHOVA]
    #25544112 - 10/17/18 09:30 AM (5 years, 3 months ago)

Nice work Fisher!!  Just getting ready to jump in to some sterilized subs.  This was perfect timing.


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OfflineSHROOMSISAY01M
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Re: PC bags without sealing [Re: van hatton]
    #25544504 - 10/17/18 12:08 PM (5 years, 3 months ago)

Quote:

van hatton said:
It will only melt the bags if the flames go up the side of the pc.

Quote:

JHOVA said:
how many 4 quart large .05 bags can you fit in your 23 quart presto? nice writeup!




I can fit four 5lb bags in my prestos. I could probably fit more but no need.




That is not true the heat from the gas flame has to go somewhere and it goes up the sides of the pot which is enough to melt bags. An electric stove has metal to metal heat transfer so much less heat goes up the side of the pot. If the flame had to go up the sides then anyone with a 23 quart presto which is the majority I would say would not have to worry about it. because the flame would never go up the sides of a pot that big.


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Invisiblevan hattonFacebookDiscord
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Re: PC bags without sealing [Re: SHROOMSISAY01]
    #25544574 - 10/17/18 12:39 PM (5 years, 3 months ago)

Uh the flames go up the side for 1 of my 4 burners unless i dial the gas flow back.

And no it isnt enough to melt pcs. The sides of my pcs are usually 100 degrees.  My electric stove kicks out way more heat than my burners. Much hotter all around


The flame has to be on the side and stay there consistently for the bags to melt. Regular proper use of a burner and pc you will never melt a bag.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlineverum subsequentis
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Re: PC bags without sealing [Re: van hatton]
    #25544596 - 10/17/18 12:47 PM (5 years, 3 months ago)

i can testify to that. i've done lot's of bags in my presto on a gas burner. as long as you don't run out of water and you keep the burner low enough (off the sides) you will be fine. unless you are using turkey bags or some shit bags off ebay. unicorn bags for the win!


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OfflineFishLevelMidnight
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Re: PC bags without sealing [Re: verum subsequentis]
    #25544765 - 10/17/18 01:46 PM (5 years, 3 months ago)

Between bags and jars I can usully get 12 qt in my PC, would be more with bags I imagine


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Offlinemushpunx
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Re: PC bags without sealing [Re: FishLevelMidnight]
    #25545039 - 10/17/18 03:28 PM (5 years, 3 months ago)

Nice write up man!

I basically do my bags the same way you do. I sometimes place a piece of aluminum foil between the folds in places so its easier to open. I throw a lil piece of Scotch tape on too so they stay folded nice.
I always wait till the PC cools all the way though with bags because they seal up nice, and I like to open the stopcock in front of my flow hood.

I used to do my bags like RR does, folding a tyvek sleeve between the gussets but once I put the stopcock valve on my AA I stopped using the tyvek.


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InvisibleJHOVA
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Re: PC bags without sealing [Re: mushpunx]
    #25545103 - 10/17/18 03:54 PM (5 years, 3 months ago)

MP if im gonna make a 50 gallon/200 quart sab for bags and noc em up with g2g or slurry does it matter if i use a tyvek sleeve or have it self seal like in this tek? What is the best method? I dont have the room nor cash for a FH :crankey:


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Offlinefoodguy
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Re: PC bags without sealing [Re: JHOVA]
    #25545212 - 10/17/18 04:57 PM (5 years, 3 months ago)

This is great timing. I was looking for other people doing this without tyvek, which I’ve never used in my bags. I used to just roll them up, and they would seal just fine, but I think the food thing. I’ll be trying this next batch.


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Offlinemushroomnate
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Re: PC bags without sealing [Re: foodguy]
    #25545238 - 10/17/18 05:09 PM (5 years, 3 months ago)

I just so happened to score a few bags recently.Gonna try this.:super:
:takingnotes:


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