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OfflineRoger_irrelevant
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Registered: 11/22/01
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Water in my agar containers
    #2533963 - 04/07/04 07:26 AM (12 years, 7 months ago)

I think this is the condensation that biult up when the jars were cooling. Now there is a small amount of water on the surface of the agar. Will this inhibit the growth of mycelium?


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OfflineEkstaza
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Re: Water in my agar containers [Re: Roger_irrelevant]
    #2534106 - 04/07/04 09:33 AM (12 years, 7 months ago)

I have had this happen and it didn't seem to do anything adverse, but I still get the sinking feeling that it can't be good.

Maybe someone more experienced can help us out on this one.


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InvisibleLoki
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Re: Water in my agar containers [Re: Roger_irrelevant]
    #2534218 - 04/07/04 10:21 AM (12 years, 7 months ago)

Happens to me if i under boil the potatoes for pda, seems
that if there isn't enought starch in the mix, then this happens.

Doesn't seem to affect growth too much, but I oftern flip the petri's
when this happens, to keep the moisture on the lid, not the agar surface.


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OfflineEmperor Reishi
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Re: Water in my agar containers [Re: Loki]
    #10742524 - 07/26/09 12:35 PM (7 years, 4 months ago)

I saw this old thread while searching about this problem. It'd happened to me a couple times now. Anybody know how to prevent the water build up on the surface of agar. Mine sometimes looks like I have bacteria on surface. Kinda clear and runny.... Is this due to not proper agar/water ratio?


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Invisiblewygram
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Re: Water in my agar containers [Re: Emperor Reishi]
    #10742677 - 07/26/09 01:14 PM (7 years, 4 months ago)

Condensation is due to pouring agar that is too hot and creating a temperature differential that causes the sweating.

Quote:

RogerRabbit said:
I let the bottle of agar cool 'almost' until room temperature.  The agar will still pour ok.  Always wait until you can at least handle the bottle of agar without any heat protection for your hands.  Pour the dishes in a vertical stack as said above.  Condensation is formed because of the difference in temperature.  Condensation will always form on the warm side of a surface, so having the dishes stacked eliminates the temperature differential between them.  What condensation forms using this method will usually be reabsorbed by the agar within a couple of days.
RR




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