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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: One success and one failure and I want to know why [Re: groovimus]
    #25338376 - 07/21/18 04:58 AM (5 years, 7 months ago)

Quote:

groovimus said:
OK guys I had two drastically different outcomes and want to know why. OK I started out with 2 sterile grain bags with self-healing patch, inoculated the first one with 1/2 syringe the golden teachers strain, and put the other bag in the freezer. The first bag turned out very well. two months laer I thawed the 2nd bag and changed my mind about inoculating and put it back in the freezer. I got it back out 3 months subsequent thawed it out and inoculated with the second half of the same syringe which had been refrigerated the whole time. The second bag generated no growth. No mycelium, no mold, no bacteria. I inoculated with another syringe from the refrigerator of another strain and no change with months gone by. Why success the first try and no mycelium the second try?




Your moisture content in your grains were probably fucked. I've never heard of anyone talking about freezing grains before. It's probably a really bad idea. Nocc them up within a day or two of sterilizing them.

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OfflineMush Hunter
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Registered: 12/10/16
Posts: 1,071
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Re: One success and one failure and I want to know why [Re: boom boom]
    #25338385 - 07/21/18 05:24 AM (5 years, 7 months ago)

Your moisture content in your grain probably crystalized it the freezer killing the nutrient in the grain. Kinda like the affect of freezer burning a steak.

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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Cultivation General Discussion [Re: Mush Hunter]
    #25338483 - 07/21/18 07:14 AM (5 years, 7 months ago)

Changing subject back.

Testing out some new jars today, and noccing up a previously cooked batch of grain and no pours as well.


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OfflineFunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 10 months
Re: Cultivation General Discussion [Re: ManifoldPrime]
    #25339320 - 07/21/18 03:50 PM (5 years, 7 months ago)

Is this Trich? Bad feeling


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InvisibleMateja
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Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: Cultivation General Discussion [Re: FunGuyZ]
    #25339406 - 07/21/18 05:04 PM (5 years, 7 months ago)

Doesn't look good at all, cover the holes or place it outside and maybe some pins will mature before the colony gives up. You'll catch em  next time!


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Cakes inside Water Tub

Edited by Mateja (07/21/18 05:04 PM)

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OfflineFunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 10 months
Re: Cultivation General Discussion [Re: Mateja]
    #25339440 - 07/21/18 05:29 PM (5 years, 7 months ago)

I removed the PolyFil from the hole just for the pic

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InvisibleMateja
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Registered: 07/14/16
Posts: 7,948
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Re: Cultivation General Discussion [Re: FunGuyZ]
    #25339449 - 07/21/18 05:34 PM (5 years, 7 months ago)

Start new projects :crazy2: gl


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Cakes inside Water Tub

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Offlinescab eater
Stranger Danger
I'm a teapot

Registered: 04/28/11
Posts: 111
Last seen: 5 years, 4 months
Re: Cultivation General Discussion [Re: Mateja]
    #25339799 - 07/21/18 10:22 PM (5 years, 7 months ago)

How do you guys prevent burst grains in wbs? I can't find the link to the tek I usually use but it's basically boil water, throw in wbs. Let sit for an hour, rinse, strain, jar pc for 90 mins. Every time i get burst seeds, they clump together and i can't get them to break apart. This causes the mycelium to never fully colonize.

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InvisibleJHOVA
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Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Cultivation General Discussion [Re: scab eater]
    #25339814 - 07/21/18 10:41 PM (5 years, 7 months ago)

you can soak in cold water 12 hours.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Offlinescab eater
Stranger Danger
I'm a teapot

Registered: 04/28/11
Posts: 111
Last seen: 5 years, 4 months
Re: Cultivation General Discussion [Re: JHOVA]
    #25339820 - 07/21/18 10:47 PM (5 years, 7 months ago)

Sweet. I used to do the 24hr soak. I'll try the 12 and see how it goes.

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InvisibleGerms
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: scab eater]
    #25344371 - 07/24/18 12:59 PM (5 years, 7 months ago)

This Verticillium infection is getting out of hand. It’s spread to other fruiting tubs now... short of going home and trashing everything, what can I do to clean up or prevent it after starting fresh? Shit happened so fast, thought I got it under control but guess not. Freshly cased PE mono is pinning and one of the chunky pins has already split its stipe

Reading online published articles about Verticillium fungicola say it can survive up to 6 months and that cleaning with water actually spreads the spores even more/farther


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: Germs]
    #25344418 - 07/24/18 01:21 PM (5 years, 7 months ago)

Damn dude. SFYL. I'd harvest then place everything thats after full colonisation outside. Double check all your plates.

After a quick search:

Quote:

I hope I never see that again... It scared the shit out of me. The peeling happened entirely over night. One day it was fine, the next morning, disaster.

MY grow room used to be in a loft above my kitchen. I attribute its uncleanliness to a serious fungal gnat problem I had while growing. I believe the gnats led to the vert, but thats just my theory. I quit growing for ~4 months until I could grow in a new room away from my kitchen.




^Munch


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
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Re: Cultivation General Discussion [Re: ManifoldPrime]
    #25344688 - 07/24/18 03:51 PM (5 years, 7 months ago)

Quote:

Germs said:
This Verticillium infection is getting out of hand. It’s spread to other fruiting tubs now... short of going home and trashing everything, what can I do to clean up or prevent it after starting fresh? Shit happened so fast, thought I got it under control but guess not. Freshly cased PE mono is pinning and one of the chunky pins has already split its stipe

Reading online published articles about Verticillium fungicola say it can survive up to 6 months and that cleaning with water actually spreads the spores even more/farther



I would do
Quote:

ManifoldPrime said:
Damn dude. SFYL. I'd harvest then place everything thats after full colonisation outside. Double check all your plates.



:whathesaid: and sterilize everything, take every sanitizer you can get your hands on to all your tubs and do bags for a few months :shrug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Germs]
    #25344990 - 07/24/18 06:06 PM (5 years, 7 months ago)

Quote:

Germs said:


Unfortunately, that Nu Muang clone culture I had posted in a shoebox does have vert riding along. Harvested this tub and trashed it. Was a decent clone for big shrooms too. Seems that the infection is parasitic in that the caps are all smaller than normal





So I kept this tub around in the corner of a different room with the lid on, knowing I shouldn’t have and being told not to do so, and ended up getting this for a second flush



Nothing shows signs of infection.
Wtf is going on?


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Germs]
    #25344998 - 07/24/18 06:09 PM (5 years, 7 months ago)

Well I actually lifted it out of the tub and put it into a different unmodded tub so air wouldn’t flow thru and pick up vert spores. Even though they’re already probably everywhere by now



This is the PE fruit that I think is splitting. The blue is from me touching it. Split overnight

Sorry for the dub post


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OfflineCrispykoot
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Registered: 10/16/16
Posts: 5,922
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Re: Cultivation General Discussion [Re: Germs]
    #25345014 - 07/24/18 06:21 PM (5 years, 7 months ago)

What about a steam cleaning and then ozone to kill the rest after the room is empty?


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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Crispykoot]
    #25345030 - 07/24/18 06:30 PM (5 years, 7 months ago)

Quote:

Crispykoot said:
What about a steam cleaning and then ozone to kill the rest after the room is empty?



That sounds like a solid idea.. gonna watch how these last few monos fruit and if its even slightly askew, the place is getting bombed.

I threw out about 80% of my stuff, save 2 cultures and 3 fresh monos. Several spawn jars went out. Only kept the mexicana and a few masters. Doing a pre-deep clean tonight

No rest for the wicked


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OfflineCanharo
Glassblowing Extraordinaire
I'm a teapot


Registered: 10/27/14
Posts: 327
Last seen: 8 months, 30 days
Re: Cultivation General Discussion [Re: Germs]
    #25345309 - 07/24/18 09:41 PM (5 years, 7 months ago)

Quote:

Germs said:
This Verticillium infection is getting out of hand. It’s spread to other fruiting tubs now... short of going home and trashing everything, what can I do to clean up or prevent it after starting fresh? Shit happened so fast, thought I got it under control but guess not. Freshly cased PE mono is pinning and one of the chunky pins has already split its stipe

Reading online published articles about Verticillium fungicola say it can survive up to 6 months and that cleaning with water actually spreads the spores even more/farther




Fuck I'm starting to wonder if this was what killed my last couple tubs, One of my APE's looked like it exploded where the veil drops and the veil was thick, like a couple millimeters.  I also just had a Xupari plate pin and the stripes pealing on it.  I trashed what I had going on but got several more jars of both APE and red boy clones, I've been cleaning the room alot.  Hopefully come Thursday when I spawn it'll be a fresh start, best of luck to you Germs
:feelsbadman:

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OfflineCrispykoot
Jello Wrangler
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Registered: 10/16/16
Posts: 5,922
Loc: Flag
Last seen: 4 days, 6 hours
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Re: Cultivation General Discussion [Re: Canharo]
    #25345548 - 07/25/18 05:10 AM (5 years, 7 months ago)



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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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OfflineLegend21
Legend
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Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 6 months
Re: Cultivation General Discussion [Re: Crispykoot]
    #25345652 - 07/25/18 07:23 AM (5 years, 7 months ago)

What do you guys do with your used agar dishes? I have some fully colonized that I wont use but Im hesitant of throwing them in the trash

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