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OfflineMinimumEfrt2
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Registered: 03/17/04
Posts: 293
Last seen: 6 years, 3 months
using regular lighters during innoculation
    #2533135 - 04/06/04 10:56 PM (12 years, 8 months ago)

can i just use a regular old bic cigarette lighter to sterilize the needle?? if not how could i make an alcohal lighter?


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InvisibleJohn
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Registered: 08/08/03
Posts: 7,026
Loc: Vancouver, B.C.
Re: using regular lighters during innoculation [Re: MinimumEfrt2]
    #2533144 - 04/06/04 10:59 PM (12 years, 8 months ago)

yeah bic will work, kills the fluid though and takes a while to get red hot.


--------------------
There's a thin line between sanity and insanity... and I just snorted it.


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Offlinedistortion
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Registered: 12/11/02
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Re: using regular lighters during innoculation [Re: MinimumEfrt2]
    #2533149 - 04/06/04 11:03 PM (12 years, 8 months ago)

AFOAF just uses a bic on the needle for 5 or 10 seconds, doesnt wait for it to get red hot. then he wipes off the black soot with a paper towel soaked with 99% isopropyl alcohol.


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OfflineMinimumEfrt2
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Registered: 03/17/04
Posts: 293
Last seen: 6 years, 3 months
Re: using regular lighters during innoculation [Re: distortion]
    #2533311 - 04/07/04 12:08 AM (12 years, 8 months ago)

would a towel soaked in rubbing alcohal work?


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Invisiblejimi
Experienced
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Registered: 11/21/99
Posts: 44
Loc: Greenwood Memorial Park
Re: using regular lighters during innoculation [Re: MinimumEfrt2]
    #2533354 - 04/07/04 12:23 AM (12 years, 8 months ago)

Home-made alcohol burner tek:

1. Buy a wick.
2. Find a small bottle with a metal lid (a baby food jar would work great).
3. Drill a hole in the lid of jar. Make the hole as small as possible, just large enough to squeeze the wick through.
4. Pull the wick up about an inch or so.

Done, but in the long run, ordering a burner would be easier/more efficient than using a bic or making your own burner. It would pretty much last forever, unless you cracked it.


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OfflinePsilygirl
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Registered: 08/28/03
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Re: using regular lighters during innoculation [Re: MinimumEfrt2]
    #2533390 - 04/07/04 12:37 AM (12 years, 8 months ago)

all i do is pass the flame of a bic lighter under the needle for like 5 seconds or so, then rub it with a paper towel in isopropyl. i usually dont wait for it to get red hot, it's not really necessary.

i work in a lab and when we flame-dry/sterilize this sort of stuff, it doesnt need to get red hot, just hot enough to burn off any residual compounds (in this case, any bacteria/spores in the air). use your own judgement, i guess, and if red hot makes you more comfortable, then go for it.

then keep the needle in an isopropyl-soaked paper towel until you are ready to use it. the less time its exposed the less chance of contam.


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"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."


Puget Sound Mycological Society


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