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Offlineshroomology702
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First grain jars from agar transfers. Is this contaminated?
    #25320787 - 07/11/18 03:20 PM (5 years, 9 months ago)

Hello to the awesome community of Shroomery!  Hoping you guys can help me out with my concern...

This is my first colonized Rye grain jar. Transfer from what looked like a very healthy and clean agar plate. It took a while for the Myc to jump of the agar wedge and onto the grain. But once it did growth exploded.

Anyway there seem to be some sections with shades of gray and yellow they are barely visible or noticeable. Is that contamination?  I was planning on transferring g2g from this jar. What do you guys think. Any feedback is welcome and thanks in advance for your advice! 




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InvisibleTheMadHatter420
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25320957 - 07/11/18 05:14 PM (5 years, 9 months ago)

I would shake it and if it has good recovery within 24 hours, then G2G, just don't stretch it to thin. You might have a little bacteria in there, but its not that much and it should be just fine doing G2G. I would do it and pull off at least 1 flush.


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: TheMadHatter420]
    #25323134 - 07/12/18 09:15 PM (5 years, 9 months ago)

Quote:

TheMadHatter420 said:
I would shake it and if it has good recovery within 24 hours, then G2G, just don't stretch it to thin. You might have a little bacteria in there, but its not that much and it should be just fine doing G2G. I would do it and pull off at least 1 flush.




Thanks for your reply MadH!  So I followed your advice and gave them a quick shake. Once the myc broke up, there were some clumps of grain stuck together with a thick white substance. Didn’t look like myc. These grains would not break up.

I’m guessing at this point it’s contamination for sure. Can anyone tell me if it is and what type of contam it is?  I’m really enjoying the process of working with this stuff and want to learn everything I can. . Never knew the wonders of this unique marvelous organism!  This forum has been my daily reading material for almost a year now. Anyway thanks in advance for your help!!!
Peace homies!


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OfflineThesunbeam
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25323149 - 07/12/18 09:27 PM (5 years, 9 months ago)

Looks clean, use it for what every you want.

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InvisibleCaps McGee
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Re: First grain jars from agar transfers. Is this contaminated? [Re: Thesunbeam]
    #25323198 - 07/12/18 10:14 PM (5 years, 9 months ago)

They'll break up: use a bicycle tire, or padded carpet to smack the side... I just use my hand, opened and cupped


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25323392 - 07/13/18 01:59 AM (5 years, 9 months ago)

Thanks sunbeam and caps. I’ll take your word for it. If they regrow after being shaken I will spawn to bulk. Hopefully I’m wrong that the white clumps are some type of contam.  It just doesn’t look like myc from my very limited experience.

Any other opinions?

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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: Caps McGee]
    #25323399 - 07/13/18 02:15 AM (5 years, 9 months ago)

Thanks sunbeam and Caps!  I did hit them on a thick padded foam workout tile. I’ll try to break it up on a tire tomorrow AM.

My bigger concern is contamination.  It looks like a very thick and dense substance. Not at all like myc I’ve seen in my very little time of messing with Rye grain and Agar. If I break it up will it just spread and grow throughout the jar? 

Is there any way to know if I should spawn to bulk of cut my losses and discard?

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InvisibleCaps McGee
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25323638 - 07/13/18 07:48 AM (5 years, 8 months ago)

Quote:

shroomology702 said:
Thanks sunbeam and Caps!  I did hit them on a thick padded foam workout tile. I’ll try to break it up on a tire tomorrow AM.

My bigger concern is contamination.  It looks like a very thick and dense substance. Not at all like myc I’ve seen in my very little time of messing with Rye grain and Agar. If I break it up will it just spread and grow throughout the jar? 

Is there any way to know if I should spawn to bulk of cut my losses and discard?



Looks normal to me... may have overcooked your grain and caused it a bit starchy... the mycelium in these clumps will look nasty  (thick, pasty almost) and won't exhibit the fluffy look until after recuperation


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: Caps McGee]
    #25325208 - 07/14/18 12:35 AM (5 years, 8 months ago)

Sounds good, Caps.  Myc seems to be recovering good after last shake. You may be right that i over cooked some grain. I’m still adjusting my process. So far had better luck no popping grain with the ROGER rabbit  grain prep video.  Still making adjustments.

Anyway, once grains re over I’ll spawn to coir/Poo/verm/gypsum. Appreciate your advice!  I’d rate you if I could. :/.

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InvisibleNumeroEno
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25325226 - 07/14/18 12:58 AM (5 years, 8 months ago)

Yeah dude it looks good. There may be some bacteria, but as a wise cultivator once told me, all jars have some bacteria. If it was bad you would know it. Sometimes slightly bacterial spawn will start to trich out towards the end of the first flush, so get what you can and if any trich pops up, well, :deathstar:


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InvisibleCaps McGee
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25326504 - 07/14/18 06:56 PM (5 years, 8 months ago)

Quote:

shroomology702 said:
Sounds good, Caps.  Myc seems to be recovering good after last shake. You may be right that i over cooked some grain. I’m still adjusting my process. So far had better luck no popping grain with the ROGER rabbit  grain prep video.  Still making adjustments.

Anyway, once grains re over I’ll spawn to coir/Poo/verm/gypsum. Appreciate your advice!  I’d rate you if I could. :/.



Why can't you rate?


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InvisibleJHOVA
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25326587 - 07/14/18 07:54 PM (5 years, 8 months ago)

post a pic of the recovery


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OfflineMadcapper144
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Re: First grain jars from agar transfers. Is this contaminated? [Re: JHOVA]
    #25326732 - 07/14/18 09:34 PM (5 years, 8 months ago)


This was one outta 18 jars few days after a shake. I think your good as long as it doesn't start looking like this lol!


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: Caps McGee]
    #25326875 - 07/14/18 11:08 PM (5 years, 8 months ago)

Quote:

Caps McGee said:
Quote:

shroomology702 said:
Sounds good, Caps.  Myc seems to be recovering good after last shake. You may be right that i over cooked some grain. I’m still adjusting my process. So far had better luck no popping grain with the ROGER rabbit  grain prep video.  Still making adjustments.

Anyway, once grains re over I’ll spawn to coir/Poo/verm/gypsum. Appreciate your advice!  I’d rate you if I could. :/.



Why can't you rate?




You have to have at least 50 posts to rate.

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InvisibleCaps McGee
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25327145 - 07/15/18 07:32 AM (5 years, 8 months ago)

I didn't realize that... Makes sense though I guess


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25327748 - 07/15/18 01:22 PM (5 years, 8 months ago)

What’s up once again everyone! 

So out of the 5 jars I transferred agar wedges to, 3 of them seem to have recovered well after the shake. The other two do show some growth but not as much as the three on the left.

Does that mean there is contamination or is it still worth it to transfer them from G2G. Maybe spawn to bulk?

Any feedback is appreciated!  Thank you!!!!


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OfflineManifoldPrime
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25327995 - 07/15/18 03:14 PM (5 years, 8 months ago)

How long ago was the shake? those rye grains look sexy


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Offlineshroomology702
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Re: First grain jars from agar transfers. Is this contaminated? [Re: ManifoldPrime]
    #25328135 - 07/15/18 04:30 PM (5 years, 8 months ago)

Quote:

ManifoldPrime said:
How long ago was the shake? those rye grains look sexy




Hey Prime. I shook the grain 2 days ago. I was informed that the recovery time was an indication of contamination. What do you think?

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InvisibleCaps McGee
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Re: First grain jars from agar transfers. Is this contaminated? [Re: shroomology702]
    #25328531 - 07/15/18 07:59 PM (5 years, 8 months ago)

Could also indicate poor grain conditions  (too dry, too wet, under-rinsed or overcooked) or poor GE... Are the filters wet?


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InvisibleJHOVA
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Re: First grain jars from agar transfers. Is this contaminated? [Re: Caps McGee]
    #25328640 - 07/15/18 08:46 PM (5 years, 8 months ago)

You g2ged bacterial grains and now it’s super bacterial


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