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OfflineWils030987
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Pf tek with honey/Karo/sugar water?
    #25306927 - 07/03/18 06:29 PM (6 years, 6 months ago)

I apologise if this has been answered before, I can’t seem to find a straight answer, only completely different recipes. My basic question is:

Can regular h2o be replaced with honey/Karo/sugar water in the pf tek? And would it be beneficial?

Reason I ask is if mycelium grows in these media in a liquid culture, and rather quickly, could it not be used to enhance the speed of the PF tek?

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OfflineLtLurker
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Re: Pf tek with honey/Karo/sugar water? [Re: Wils030987]
    #25306937 - 07/03/18 06:35 PM (6 years, 6 months ago)

I think you'd only be giving the myc's competition more food.

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OfflineWils030987
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Re: Pf tek with honey/Karo/sugar water? [Re: LtLurker]
    #25307460 - 07/04/18 01:35 AM (6 years, 6 months ago)

Potentially. But in theory would it beneficial?

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Invisibleplurfekt
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Re: Pf tek with honey/Karo/sugar water? [Re: Wils030987]
    #25307565 - 07/04/18 04:21 AM (6 years, 6 months ago)

I've had a lot of success with organic-sugar-dH2O only LC's, for years.  That's Turbinado and Distilled water.

I have access to better materials now (dextrose, light malt extract) but have been finding the mycelium likes a tiny dash of sugar to boost growth initially, until the dextrose and LME takes off.  Both end up looking really good but the ones with a dash of sugar make it there faster, I plan on doing a pictorial with a clone.

That being said...you don't even need anything but organic sugar.  Doesn't even have to be turbinado brand, they are just conveniently packaged in pre-weighed packets.

https://www.shroomery.org/forums/showflat.php/Number/12412908#12412908
https://www.shroomery.org/forums/showflat.php/Number/6378527/fpart/1/vc/1

The one thing I will say about the turbinado tek is tone down the amount of sugar you use, or it will hault growth.

I find about 1/3 of a packet of turbinado per 125ml is plenty.  I think each packet is 5g pre-measured, IIRC.

If you're going to make 500ml, I'd use one packet or less worth of organic sugar.



You'd be better off with some other stuff in there but it'll work, just make sure your sterile procedure is up to par.

(Oh and to answer the basic question of will a liquid culture work better and faster than MS spore solution - absolutely...if it is clean!)

But then you have to factor the time lost waiting for the LC to colonize, if you have no cakes at all, or patience, you'd be better off with just spores IMO.

Then again, it's fucking awesome to watch a PF cake colonize in a week because you blasted it with thick LC.


I always used the LC, but I was never in a race.  Also, sugar only is old tek (old technology) and there are alternatives on making better ones these days, but since all I use is premium stuff I'll leave the "potato and/or other grain straining" for someone else to fill you in on.

Good luck

:mushroom2:

Edited by plurfekt (07/04/18 04:43 AM)

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OfflineGeinstein
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Re: Pf tek with honey/Karo/sugar water? [Re: plurfekt]
    #25307571 - 07/04/18 04:28 AM (6 years, 6 months ago)

OK will first of it can work in theory but contam risks are way to high look there is a reason no one uses spores in lc anymore because . . .because spores are never 100%clean. . .never end of story.
OK now if you want to add some more nuts to it fro liquids use between 50% to 25% strength coffee.
Don't use coffee grounds in your PF cakes because the BRF will have more nuts for the mycelium the extra nuts from the coffee will give it a nice boost with out compromising the cakes more important sources of nutrition.


--------------------


Nothing breads nothing

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Invisibleplurfekt
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Re: Pf tek with honey/Karo/sugar water? [Re: Geinstein]
    #25307575 - 07/04/18 04:33 AM (6 years, 6 months ago)

Quote:

Geinstein said:
OK will first of it can work in theory but contam risks are way to high look there is a reason no one uses spores in lc anymore because . . .because spores are never 100%clean. . .never end of story.
OK now if you want to add some more nuts to it fro liquids use between 50% to 25% strength coffee.
Don't use coffee grounds in your PF cakes because the BRF will have more nuts for the mycelium the extra nuts from the coffee will give it a nice boost with out compromising the cakes more important sources of nutrition.




I still put spores in LC's, until I get fruits to clone or solid isolates to test.

My spores must be clean as fuck.

I would never recommend doing this with anything but a professionally made spore syringe from either a member well respected for his printing capabilities or a straight up vendor.  I've never had a syringe fail from a Shroomery sponsor.

Again, this is old tek, but he's asking whether or not it works, hes not asking whether or not it's the best method.

It most definitely works, if it's clean!

There are most definitely better ways to go about this.  Look into no-pour agar teks, there is an abundance of them around.  Pasty and Josex leading the show on that one.


Nothing says you can't inoculate the LC, then knock up some jars with the same syringe and not leave all your eggs in one basket.

Spores don't get old fast, either.

Edited by plurfekt (07/04/18 04:38 AM)

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OfflineWils030987
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Re: Pf tek with honey/Karo/sugar water? [Re: plurfekt]
    #25307605 - 07/04/18 05:12 AM (6 years, 6 months ago)

Thanks guys. Lots to consider

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Invisibleplurfekt
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Re: Pf tek with honey/Karo/sugar water? [Re: Wils030987]
    #25307610 - 07/04/18 05:17 AM (6 years, 6 months ago)

Quote:

Wils030987 said:
Thanks guys. Lots to consider




Yea my general advice would be don't overwhelm yourself if you are really new to this.

If you are hell-bent on wanting to use an LC now, you could.  But it is still going to be multi-spore the same as the spore solution.

Also, the amount of time it will take for the LC to colonize and then inoculate the PF cakes until they reach full colonization will be faster if you just start from spores.

However, if you make some LC now, then do your first set of cakes with the remaining spore solution, your next cakes can be inoculated with that LC for faster colonization, at which point you can take some spore prints so you never run out, all without slowing down your current progress.

Time management wise, that is the most logical way to go & is what I would do in your position without any agar experience, unless that becomes overwhelming - just stick to the PF Tek.

Last thing you want is to feel like this hobby is too much work, because it's not.  Work smarter, not harder.

Best wishes!

:thumbup:

Edited by plurfekt (07/04/18 05:25 AM)

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InvisiblemushboyMDiscord
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Re: Pf tek with honey/Karo/sugar water? [Re: Wils030987]
    #25307643 - 07/04/18 05:48 AM (6 years, 6 months ago)

Quote:

Wils030987 said:
Potentially. But in theory would it beneficial?




No. Living things like straight up water. What happens to people who are fed sugar water?

Fat, weak, slow, sluggish. Putting complex sugar into LCs will also do this. Avoid it. So no honey, sugar/karo broths:nono:

If you have brf from pktek, use 1-2g per 500ml water. Unfiltered. Cheap, easy.

Use agar only. ANY spore to LC is like not wearing a seatbelt because, 'nothing bad has happened yet!' At some point you will go thru the windshield.

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OfflineLtLurker
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Re: Pf tek with honey/Karo/sugar water? [Re: mushboy]
    #25307825 - 07/04/18 08:34 AM (6 years, 6 months ago)

Quote:

mushboy said:
Quote:

Wils030987 said:
Potentially. But in theory would it beneficial?




No. Living things like straight up water. What happens to people who are fed sugar water?

Fat, weak, slow, sluggish. Putting complex sugar into LCs will also do this. Avoid it. So no honey, sugar/karo broths:nono:

If you have brf from pktek, use 1-2g per 500ml water. Unfiltered. Cheap, easy.

Use agar only. ANY spore to LC is like not wearing a seatbelt because, 'nothing bad has happened yet!' At some point you will go thru the windshield.



:whathesaid:

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